Abstract: A self-acting dredging and drainage structure for a plain river network irrigation area is provided. The structure includes a first tube body, a water inlet end side of the first tube body is provided with a trash rack, a partition body is provided in the first tube body, and a water outlet end side of the first tube body is provided with a contraction and diversion device. The contraction and diversion device divides a water outlet end of the first tube body into an upper water outlet side and a lower water outlet side, and the contraction and diversion device includes no less than one arranged contraction and diversion pipe; and the upper water outlet side has first water outlets and second water outlets.

Abstract: The application relates to a run-length stripping method for generating skeleton lines of complex plain river networks, including following steps: 1. constructing an empty raster field, each raster row generates an empty run-length linked list; 2. completing raster compression on each row, and compressing the continuous grids into run-length units and inserting them into the raster row run-length linked list; 3. establishing a raster field river boundary grid search template; 4. setting two discriminant functions to judge whether a grid on a raster field is a redundant grid; 5. searching all boundary grids along a raster field boundary, and marking and stripping all redundant grids based on two discriminant functions respectively; 6. analyzing whether the boundary grid is stripped, if so, returning to continue stripping, otherwise, entering the next step; 7. converting the non-stripped grid into skeleton line data in vector form.

Abstract: The application relates to a run-length stripping method for generating skeleton lines of complex plain river networks, including following steps: 1. constructing an empty raster field, each raster row generates an empty run-length linked list; 2. completing raster compression on each row, and compressing the continuous grids into run-length units and inserting them into the raster row run-length linked list; 3. establishing a raster field river boundary grid search template; 4. setting two discriminant functions to judge whether a grid on a raster field is a redundant grid; 5. searching all boundary grids along a raster field boundary, and marking and stripping all redundant grids based on two discriminant functions respectively; 6. analyzing whether the boundary grid is stripped, if so, returning to continue stripping, otherwise, entering the next step; 7. converting the non-stripped grid into skeleton line data in vector form.

Abstract: A method for construction of a medium-throughput single-cell copy number sequencing (MT-scCNV-seq) library and sequencing includes: delivering single cells each to a tube, and independently lysing each cell; labeling each cell with a cell-specific barcode while tagmenting the gDNA with an innovative Tn5 transposome; pooling the reactions of a plurality of cells simultaneously treated above, and constructing a batch of sequencing libraries for the cells collectively in a single tube with primers containing a batch index. The specific tagmentation of the gDNA of a given cell by the Tn5 transposome enables early pooling of multiple cells in a single tube for collective library construction, without pre-whole-genome-amplification of each cell. The output library is compatible with a conventional NGS platform and program. Finally the sequencing data is disaggregated, and traced to each cell according to the barcode and index; the CNV profile for each cell of the panel is accurately identified.

Abstract: The invention discloses a primer set and a multiplexable library building scheme for constructing an RNA sequencing library with a related reagent kit and an application thereof. The invention also discloses a corresponding data analysis method and a related instrument. The primer set used for RNA sequencing library construction includes the reverse transcription primers containing poly-dT, the 2nd strand cDNA primers particularly contain a random or semi-random portion at their 3? end for universal initiation of the synthesis at multiple sites, or sequence capturing the 1st strand of a specific cDNA, as well as their corresponding PCR primer 1 and 2. By this method, the library construction process is simple and the operation is convenient; the time of building the database was significantly shortened; it can carry out a large number of samples in a single run; the analysis process is simpler; the cost of database building, sequencing and analysis is significantly reduced.

Abstract: A portable self-timer rack comprises a telescoping mechanism and a magnetic adsorption portion connected with the telescoping mechanism in a rotary manner. The magnetic adsorption portion comprises a box body and a magnet arranged in the box body, and the telescoping mechanism is connected with the box body through a rotary joint in a rotary manner and a wireless communication module is arranged in the telescoping mechanism.

Abstract: A portable self-timer rack comprises a telescoping mechanism and a magnetic adsorption portion connected with the telescoping mechanism in a rotary manner. The magnetic adsorption portion comprises a box body and a magnet arranged in the box body, and the telescoping mechanism is connected with the box body through a rotary joint in a rotary manner and a wireless communication module is arranged in the telescoping mechanism.