Abstract: The invention relates to the field of plant genetic engineering. In particular, the invention relates to a method and system for multiplex genome editing suitable for plants, especially crops. More particularly, the invention relates to a CRISPR nickase-based system and method, which can simultaneously carry out different types of genome editing.

Abstract: The present invention provides a method for improving plant genetic transformation and gene editing efficiency. Specifically, the method improves the regeneration efficiency of plant genetic transformation and/or improves the efficiency of plant gene editing by expressing genes that promote plant cell division, especially meristematic cell division.

Abstract: The present invention relates to the field of genetic engineering. In particular, the present invention relates to a genome editing method with high efficiency and high specificity. More specifically, the present invention relates to a method for increasing the efficiency of site-directed modification of a target sequence in a genome of an organism by a high-specificity Cas9 nuclease variant.

Abstract: Provided relates to the field of genetic engineering. In particular, a method for base editing in plant is provided. More specifically, a highly efficient A to G base editing method for a target sequence in a genome of a plant (e.g., a crop plant) by a guide RNA-directed CRISPR-adenine deaminase fusion protein and the plant and their progeny produced by the method are provided.

Abstract: The present invention belongs to the field of gene editing. In particular, the present invention relates to an improved cytosine base editing system which has a significantly reduced genome-wide off target effect and a narrow editing window.

Abstract: The present invention discloses a method for obtaining glyphosate-resistant rice by a site-directed nucleotide substitution, and also relates to a method capable of generating a site-directed nucleotide substitution and a fragment substitution. The method for obtaining a glyphosate-resistant plant provided by the present invention comprises the following steps: only substituting threonine (T) at position 8 of the amino acid sequence of a conserved region of endogenous EPSPS protein of a target plant with isoleucine (I), and substituting proline (P) at position 12 with serine (S) to obtain a plant, i.e., a glyphosate-resistant plant. The method provided by the present invention is of great significance in breeding new herbicide-resistant plant varieties.

Abstract: The present invention belongs to the field of gene editing, and particularly relates to a method and means for detecting genome wide random off-target effect of a base editing system in a rapid and high-throughput mode.

Abstract: A method of modifying an upstream open reading frame (uORF) by genome editing technique to regulate gene expression.

Abstract: Provided are an improved genome editing system and a method that has high specificity, which are capable of obtaining stable mutation types. The system includes an expression construct including a coding sequence of a gRNA targeting at least one genomic target sequence; an expression construct including a coding sequence of a CRISPR nuclease; and an expression construct including a coding sequence for a gRNA targeting a target sequence within the coding sequence of the CRISPR nuclease. Upon introduction into the cell, the gRNA targeting the at least one genomic target sequence directs the CRISPR nuclease to the at least one genomic target sequence and results in one or more mutations in the genomic target sequence, and the gRNA targeting a target sequence within the coding sequence of the CRISPR nuclease directs the CRISPR nuclease to the target sequence within the coding sequence of the CRISPR nuclease and results in an inactivating mutation of the CRISPR nuclease.

Abstract: The present invention relates to the field of plant genetic engineering. In particular, the present invention relates to acetolactate synthase (ALS) mutants, protoporphyrinogen oxidase (PPO) mutants, acetyl-CoA carboxylase (ACCase) mutants and/or p-hydroxyphenylpyruvate dioxidase (HPPD) mutants capable of conferring herbicide resistance in plants, especially in wheat and/or rice plants, and methods for production herbicide-resistant plants, especially wheat and/or rice plants comprising said acetolactate synthase (ALS) mutants and/or protoporphyrinogen oxidase (PPO) mutants and/or acetyl-CoA carboxylase (ACCase) mutants and/or p-hydroxyphenylpyruvate dioxidase (HPPD) mutants.

Abstract: The present invention belongs to the field of genetic engineering. Specifically, the present invention relates to a directed evolution method based on geminivirus. More specifically, the present invention relates to a directed evolution method for in vivo screening of a genetic element in a plant cell by using primary and secondary replicons of geminivirus.

Abstract: The present invention belongs to the field of plant genetic engineering. Specifically, the invention relates to a method for base editing in plants. More particularly, the invention relates to a method for performing efficient base editing to a target sequence in the genome of a plant (such as a crop plant) by a Cas9-cytidine deaminase fusion protein, as well as plants produced through said method and progenies thereof.

Abstract: The present invention belongs to the field of plant genetic engineering. In particular, the present invention relates to a method for obtaining wheat with increased resistance to powdery mildew, wherein the wheat has comparable or even increased yield comparable to wild type.

Abstract: The present invention relates to the field of plant genetic engineering. Specifically, the present invention relates to a method for targeted modification of a plant genome sequence. More specifically, the present invention relates to a method for targeted modification of a specific sequence in the plant genome into a target sequence of interest by a nuclease-reverse transcriptase fusion protein guided by a guide RNA, a genetically-modified plant produced by said method, and progenies of said plant.

Abstract: The present invention belongs to the field of genetic engineering. Specifically, the present invention relates to a gene editing system derived from Flavobacterium and uses thereof.

Abstract: The present invention discloses a method for site-directed modification of whole plant through gene transient expression. The method as provided for conducting site-directed modification to a target fragment of a target gene in a whole plant comprises the following steps: transiently expressing a sequence-specific nuclease in said plant, wherein a whole plant is used as the subject for transient expression, said sequence-specific nuclease targets and cleaves said target fragment, thereby the site-directed modification is achieved via the self DNA repairing of said plant.

Abstract: The present invention belongs to the field of plant genetic engineering. Specifically, the invention relates to a method for base editing in plants. More particularly, the invention relates to a method for performing efficient base editing to a target sequence in the genome of a plant (such as a crop plant) by a Cas9-cytidine deaminase fusion protein, as well as plants produced through said method and progenies thereof.

Abstract: The present invention belongs to the field of plant genetic engineering. Specifically, the invention relates to a method for creating novel herbicide resistant plants by base editing techniques and a method for screening endogenous gene mutation sites capable of conferring herbicide resistance in plants. The invention also relates to the use of the identified endogenous gene mutantation sites in crop breeding.

Abstract: The present invention discloses a method for site-directed modification of whole plant through gene transient expression. The method as provided for conducting site-directed modification to a target fragment of a target gene in a whole plant comprises the following steps: transiently expressing a sequence-specific nuclease in said plant, wherein a whole plant is used as the subject for transient expression, said sequence-specific nuclease targets and cleaves said target fragment, thereby the site-directed modification is achieved via the self DNA repairing of said plant.

Abstract: Provided is a gene editing system for editing a target sequence in the genome of a cell, comprising a CRISPR nuclease, a cytosine deaminase, an AP lyase, a guide RNA and optionally an uracil-DNA glycosylase. Also provided are a method of producing a genetically modified cell, and a kit comprising the gene editing system.