Abstract: The present disclosure relates to the technical field of biomedicine, in particular to use of advanced platelet-rich fibrin (A-PRF) in preparing a medicament for the treatment of diabetic foot ulcer. The A-PRF provided by the present disclosure has a loose reticular fibrin structure, can be rich in more platelets and leukocytes, can release a plurality of growth factors and cytokines more persistently, and is more beneficial to tissue regeneration and wound repair. The medicament prepared from the A-PRF shortens the healing period of the diabetic foot ulcer, improves the healing rate, and has an excellent treatment effect and high safety.