Patents by Inventor CaiZhe ZHANG

CaiZhe ZHANG has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Single-cell factory for efficiently synthesizing ?-aminobutyric acid and construction and application thereof
    Patent number: 10612054
    Abstract: The present invention discloses a single-cell factory for efficiently synthesizing ?-aminobutyric acid and construction and application thereof, which belong to the technical field of microorganisms. The present invention expresses an L-threonine deaminase gene, an L-amino acid dehydrogenase gene and a dehydrogenase gene for providing cofactor NADH cycle in tandem to construct a recombinant Escherichia coli single-cell factory which is used for efficiently synthesizing ?-aminobutyric acid. The expression level of the L-threonine deaminase is optimized and controlled by an RBS sequence, so that the problem of transformation inhibition caused by the rapid accumulation of an intermediate product ketobutyric acid is solved, moreover, the expression level of the dehydrogenase for providing cofactor NADH cycle is optimized and controlled by a promoter and an RBS sequence, consequently, the NADH regeneration rate is increased, and ultimately, yield is increased.
    Type: Grant
    Filed: November 17, 2016
    Date of Patent: April 7, 2020
    Assignee: JIANGNAN UNIVERSITY
    Inventors: Zhiming Rao, Junping Zhou, Taowei Yang, Xian Zhang, Meijuan Xu, CaiZhe Zhang, Yunlong Qi, Junxian Zheng
  • Single-Cell Factory for Efficiently Synthesizing alpha-aminobutyric Acid and Construction and Application thereof
    Publication number: 20190161779
    Abstract: The present invention discloses a single-cell factory for efficiently synthesizing ?-aminobutyric acid and construction and application thereof, which belong to the technical field of microorganisms. The present invention expresses an L-threonine deaminase gene, an L-amino acid dehydrogenase gene and a dehydrogenase gene for providing cofactor NADH cycle in tandem to construct a recombinant Escherichia coli single-cell factory which is used for efficiently synthesizing ?-aminobutyric acid. The expression level of the L-threonine deaminase is optimized and controlled by an RBS sequence, so that the problem of transformation inhibition caused by the rapid accumulation of an intermediate product ketobutyric acid is solved, moreover, the expression level of the dehydrogenase for providing cofactor NADH cycle is optimized and controlled by a promoter and an RBS sequence, consequently, the NADH regeneration rate is increased, and ultimately, yield is increased.
    Type: Application
    Filed: November 17, 2016
    Publication date: May 30, 2019
    Inventors: Zhiming RAO, Junping ZHOU, Taowei YANG, Xian ZHANG, Meijuan XU, CaiZhe ZHANG, Yunlong QI, Junxian ZHENG