Abstract: The present disclosure describes a system and methods for positioning a configured number of biomaterials on a surface. The method disclosed includes the formation of a membrane mask structure including a configured number of nanopores. The nanopores are generally sized to be the same size as, or slightly larger than a single target biomaterial. The masking membrane is then adhered to a substrate. The substrate may include a raw substrate, or may be functionalized for the purpose of binding biomaterials with greater attraction. A solution including the target biomaterials is then exposed to the masking membrane. One biomaterial is able to adhere to the substrate through each nanopore. The solution is rinsed from the membrane surface leaving only the biomaterial that has adhered to the substrate with a relatively strong binding force. The masking membrane may then either remain or may be removed.