Abstract: A locking device for a tubing connection used in intravenous feeding. The invention comprises a body with a channel therein adapted to receive a tubing connection. The body is resiliently compressible transverse to the channel. To secure the locking device against a tubing connection, the body is compressed and a retainer installed. Once in place, the locking device prevents separation of the intravenous tubing connection between a vein insert tube and the termination of an intravenous supply tube. Installation and removal of the locking device is readily effected, thus permitting secure connection and ready separation of an intravenous fluid line without pulling or twisting.