Abstract: Described herein are compositions and methods of CRISPR effector system mediated detection of targets, including, but not limited to viral targets. Also described herein are devices and kits for carrying out the CRISPR effector system mediated nucleic acid detection assays.

Abstract: A superconductor device includes a high superconductivity transition temperature enhanced from the raw material transition temperature. The superconductor device includes a matrix material and a core material. The enhancing matrix material and the core material together create a system of strongly coupled carriers. A plurality of low-dimensional conductive features can be embedded in the matrix. The low-dimensional conductive features (e.g., nanowires or nanoparticles) can be conductors or superconductors. An interaction between electrons of the low-dimensional conductive features and the enhancing matrix material can promote excitations that increase a superconductivity transition temperature of the superconductor device.

Abstract: High throughput methods utilizing spatially segregated detection systems provide a robust CRISPR-based diagnostic enabling highly sensitive detection of both DNA and RNA target molecules, with applications in multiple scenarios in human health including, for example, viral detection. Kits comprising the systems allow for point-of care applications with high-throughput processing of samples.

Abstract: An apparatus, method, computer program product, and/or system are described that determine an event, actuate a cushioning element in response to the determining the event, the cushioning element including one or more tension-bearing members, and dissipate at least some of an energy associated with a collision based on deforming at least one of the tension-bearing members during the collision, the deforming including substantially inelastically stretching the at least one of the tension-bearing members. Other example embodiments are also provided relating to actuatable cushioning elements.

Abstract: RNA targeting proteins are utilized to provide a robust massively multiplexed CRISPR-based diagnostic by detection in droplets with attomolar sensitivity. Detection of both DNA and RNA with comparable levels of sensitivity at nanoliter volumes can differentiate targets from non-targets based on single base pair differences, with applications in multiple scenarios in human health including, for example, viral detection, bacterial strain typing, and sensitive genotyping.

Abstract: Methods for generating primers and/or probes for use in analyzing a sample which may comprise a pathogen target sequence are provided, including identifying pan-viral sets of primers and/or probes.

Abstract: The embodiments disclosed herein utilize RNA targeting effectors to provide a robust CRISPR-based diagnostic for hemorrhagic fever virus applications. Embodiments disclosed herein can differentiate between hemorrhagic fever viruses that present with similar symptoms, as well as between strains of a hemorrhagic fever virus.

Abstract: The invention provides for methods for designing sensitive, specific, and optimally active binding molecules. Systems, methods and compositions utilizing the designed molecules in diagnostics and therapeutics are also provided.

Abstract: In one embodiment, a particular state of a body is sensed. In response to the sensing, at least one action is taken to modulate a projected adverse interaction between the body or a portion thereof and at least one object in the environment of the body. An apparatus, methods and computer program product, and system are described that enable a first subset of actuatable cushioning elements for a first time period, enable a second subset of actuatable cushioning elements for a second time period, determine an event, and actuate, based on a time the event is determined, at least one of the first and the second subsets of actuatable cushioning elements to provide cushioning support for an object. Other example embodiments are also provided relating to actuatable cushioning elements.

Abstract: Provided herein is a nucleic acid detection system comprising: a CRISPR system comprising an effector protein and one or more guide RNAs designed to bind to corresponding target molecules; an RNA-based masking construct; and optionally, nucleic acid amplification reagents to amplify target RNA molecules in a sample. In another aspect, the embodiments provide a polypeptide detection system comprising: a CRISPR system comprising an effector protein and one or more guide RNAs designed to bind a trigger RNA, an RNA-based masking construct; and one or more detection aptamers comprising a masked RNA polymerase promoter binding site or a masked primer binding site. In some embodiments, the system may be used to detect viruses in samples.

Abstract: The present invention offers a new approach for highly multiplexed, programmable antiviral therapies that directly target viral RNA, and can be flexibly adapted to target novel viruses or emerging outbreak pathogens. Class 2, type VI CRISPR system-based therapies can be used in combination with existing antiviral compounds for viruses where such compounds exist, either by increasing their efficacy or by preventing the evolution of specific drug resistance mutations. Perhaps most excitingly, if a virus evolves resistance to a specific guide RNA sequence, it is easy to switch to a different guide RNA sequence, or to design a new guide sequence to target the new mutation. Such approaches should prevent the widespread development of resistance to Class 2, type VI CRISPR system-based therapies.

Abstract: The present disclosure relates to nanostructures assembled from nucleic acid consisting of a single strand of DNA rationally-designed to self-assemble into a hairpin loop, helical domains, and locking domains.

Abstract: A superconductor device includes a high superconductivity transition temperature enhanced from the raw material transition temperature. The superconductor device includes a matrix material and a core material. The enhancing matrix material and the core material together create a system of strongly coupled carriers. A plurality of low-dimensional conductive features can be embedded in the matrix. The low-dimensional conductive features (e.g., nanowires or nanoparticles) can be conductors or superconductors. An interaction between electrons of the low-dimensional conductive features and the enhancing matrix material can promote excitations that increase a superconductivity transition temperature of the superconductor device.

Abstract: Disclosed embodiments include methods, computer program products, and systems. Given by way of example only and not of limitation, in various embodiments a method includes: determining an event; actuating a cushioning element in response to the determining the event, the cushioning element including one or more tension-bearing members; and dissipating at least some of an energy associated with a collision based on deforming at least one of the tension-bearing members during the collision, the deforming including substantially inelastically stretching the at least one of the tension-bearing members.

Abstract: The invention provides SST motifs of controlled size and shape, comprised of a plurality of oligonucleotides, and methods for their synthesis. The motifs are formed, at least in part, by the self-assembly of single stranded oligonucleotides into curved, corrugated or twisted structures. The location of each oligonucleotide in the resultant motif is known. Accordingly, the motifs may be modified with specificity.

Abstract: The present disclosure relates to nanostructures assembled from nucleic acid consisting of a single strand of DNA rationally-designed to self-assemble into a hairpin loop, helical domains, and locking domains.

Abstract: A traceable cement mixture includes a volume of cementitious material, a plurality of taggants disposed within the volume of cementitious material according to a taggant mix ratio that is specified based on a quality of the cementitious material, and a volume of an additional material mixed with the volume of cementitious material according an additional material mix ratio.

Abstract: In one embodiment, a particular state of a body is sensed. In response to the sensing, at least one action is taken to modulate a projected adverse interaction between the body or a portion thereof and at least one object in the environment of the body. An apparatus, methods and computer program product, and system are described that enable a first subset of actuatable cushioning elements for a first time period, enable a second subset of actuatable cushioning elements for a second time period, determine an event, and actuate, based on a time the event is determined, at least one of the first and the second subsets of actuatable cushioning elements to provide cushioning support for an object. Other example embodiments are also provided relating to actuatable cushioning elements.

Abstract: A traceable cement mixture includes a volume of cementitious material and a taggant disposed within the volume of cementitious material. The taggant includes data relating to a characteristic of the volume of cementitious material.

Abstract: A traceable cement mixture includes a volume of cementitious material and a taggant disposed within the volume of cementitious material. The taggant includes data relating to a characteristic of the volume of cementitious material.