Abstract: The invention provides a micro scaled method and system for separating a subgroup of cells and/or particles from a mixture of different types of cells and/or particles in a suspension. The suspension is forced under pressure into an inlet and further into a pre-alignment channel wherein the suspension is subjected to a two dimensional acoustic force directed perpendicular to the length direction of the pre-alignment channel. The suspension is then forced into a second inlet and further into at least one separation channel wherein the mixture of cells and/or particles are forced towards the walls of the channel and subjected to a one dimensional acoustic force directed perpendicular to the length direction of the separation channel. Cells and/or particles having the same size and/or mass density and/or compressibility are collected through at least two outlets.

Abstract: The disclosure related to a method and system to separate a subgroup of objects from a mixture of objects present in a suspension wherein said suspension if exposed to acoustic forces acting in one or two dimensions, which forces said objects to separate from each other into groups which have similar properties in connection with size and/or mass density and/or compressibility.