Abstract: An automatically operating boat locking assembly includes a spring-loaded bolt which engages through the bow eye of a boat to secure the boat on a trailer and which can be withdrawn to free the boat for removal from the trailer. The bolt can be rotated in its withdrawn position to engage a portion of a frame of the assembly which prevents return of the bolt to the extended locking position. A trigger member carried by a frame of the locking assembly is contacted by the bow eye of the boat as the boat approaches the trailer and rotates the bolt whereupon the spring associated therewith returns the bolt to the locking position, through the bow eye locking the boat on the trailer.