Patents by Inventor Carl O. Pabo
Carl O. Pabo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9289451Abstract: Disclosed herein are methods and compositions for targeted cleavage of a genomic sequence, targeted alteration of a genomic sequence, and targeted recombination between a genomic region and an exogenous polynucleotide homologous to the genomic region. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain and polynucleotides encoding same. Methods for targeted cleavage include introduction of such fusion proteins, or polynucleotides encoding same, into a cell. Methods for targeted recombination additionally include introduction of an exogenous polynucleotide homologous to a genomic region into cells comprising the disclosed fusion proteins.Type: GrantFiled: March 15, 2013Date of Patent: March 22, 2016Assignee: Sangamo BioSciences, Inc.Inventors: Fyodor Urnov, Michael C. Holmes, Jeffrey C. Miller, Carl O. Pabo
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Patent number: 8524500Abstract: Disclosed herein are methods and compositions for targeted cleavage of a genomic sequence, targeted alteration of a genomic sequence, and targeted recombination between a genomic region and an exogenous polynucleotide homologous to the genomic region. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain and polynucleotides encoding same. Methods for targeted cleavage include introduction of such fusion proteins, or polynucleotides encoding same, into a cell. Methods for targeted recombination additionally include introduction of an exogenous polynucleotide homologous to a genomic region into cells comprising the disclosed fusion proteins.Type: GrantFiled: July 16, 2010Date of Patent: September 3, 2013Assignee: Sangamo BioSciences, Inc.Inventors: Fyodor Urnov, Michael C. Holmes, Jeffrey C. Miller, Carl O. Pabo
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Patent number: 7943731Abstract: The invention provides nonnaturally occurring dimerizing peptides, and methods for their production. Such peptides are useful to mediate association of linked functional proteins domains. In particular, such peptides are useful for mediating association of complexes of multiple zinc finger proteins thereby affording greater specificity and/or affinity in binding of the zinc finger proteins to proximately spaced target segments.Type: GrantFiled: August 10, 2000Date of Patent: May 17, 2011Assignee: Massachusetts Institute of TechnologyInventors: Bryan S. Wang, Carl O. Pabo
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Patent number: 7928195Abstract: Polynucleotides encoding chimeric proteins, and methods for their production and use are disclosed. The chimeric proteins comprise a flexible linker between two zinc finger DNA-binding domains, wherein the linker contains eight or more amino acids between the second conserved histidine residue of the carboxy-terminal zinc finger of the first domain and the first conserved cysteine residue of the amino-terminal zinc finger of the second domain.Type: GrantFiled: August 13, 2009Date of Patent: April 19, 2011Assignee: Massachusetts Institute of TechnologyInventors: Jin-Soo Kim, Carl O. Pabo
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Patent number: 7888121Abstract: Disclosed herein are methods and compositions for targeted cleavage of a genomic sequence, targeted alteration of a genomic sequence, and targeted recombination between a genomic region and an exogenous polynucleotide homologous to the genomic region. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain and polynucleotides encoding same. Methods for targeted cleavage include introduction of such fusion proteins, or polynucleotides encoding same, into a cell. Methods for targeted recombination additionally include introduction of an exogenous polynucleotide homologous to a genomic region into cells comprising the disclosed fusion proteins.Type: GrantFiled: August 6, 2004Date of Patent: February 15, 2011Assignee: Sangamo Biosciences, Inc.Inventors: Fyodor Urnov, Michael C. Holmes, Jeffrey C. Miller, Carl O. Pabo
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Publication number: 20110030076Abstract: Disclosed herein are methods and compositions for targeted cleavage of a genomic sequence, targeted alteration of a genomic sequence, and targeted recombination between a genomic region and an exogenous polynucleotide homologous to the genomic region. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain and polynucleotides encoding same. Methods for targeted cleavage include introduction of such fusion proteins, or polynucleotides encoding same, into a cell. Methods for targeted recombination additionally include introduction of an exogenous polynucleotide homologous to a genomic region into cells comprising the disclosed fusion proteins.Type: ApplicationFiled: July 16, 2010Publication date: February 3, 2011Inventors: Fyodor Urnov, Michael C. Holmes, Jeffrey C. Miller, Carl O. Pabo
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Publication number: 20110014675Abstract: Polynucleotides encoding chimeric proteins, and methods for their production and use are disclosed. The chimeric proteins comprise a flexible linker between two zinc finger DNA-binding domains, wherein the linker contains eight or more amino acids between the second conserved histidine residue of the carboxy-terminal zinc finger of the first domain and the first conserved cysteine residue of the amino-terminal zinc finger of the second domain.Type: ApplicationFiled: August 13, 2009Publication date: January 20, 2011Applicant: Massachusetts Institute of TechnologyInventors: Jin-Soo Kim, Carl O. Pabo
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Patent number: 7763446Abstract: Chimeric proteins containing composite DNA-binding regions are disclosed together with DNA constructs encoding them, compositions containing them and applications in which they are useful.Type: GrantFiled: December 10, 2008Date of Patent: July 27, 2010Assignee: Massachusetts Institute of TechnologyInventors: Joel L. Pomerantz, Phillip A. Sharp, Carl O. Pabo
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Patent number: 7595376Abstract: Polynucleotides encoding chimeric proteins, and methods for their production and use are disclosed. The chimeric proteins comprise a flexible linker between two zinc finger DNA-binding domains, wherein the linker contains eight or more amino acids between the second conserved histidine residue of the carboxy-terminal zinc finger of the first domain and the first conserved cysteine residue of the amino-terminal zinc finger of the second domain.Type: GrantFiled: December 14, 2006Date of Patent: September 29, 2009Assignee: Massachusetts Institute of TechnologyInventors: Jin-Soo Kim, Carl O. Pabo
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Publication number: 20090100535Abstract: Chimeric proteins containing composite DNA-binding regions are disclosed together with DNA constructs encoding them, compositions containing them and applications in which they are useful.Type: ApplicationFiled: December 10, 2008Publication date: April 16, 2009Applicant: Massachusetts Institute of TechnologyInventors: Joel L. Pomerantz, Phillip A. Sharp, Carl O. Pabo
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Patent number: 7485441Abstract: Chimeric proteins containing composite DNA-binding regions are disclosed together with DNA constructs encoding them, compositions containing them and applications in which they are useful.Type: GrantFiled: March 7, 2006Date of Patent: February 3, 2009Assignee: Massachusetts Institute of TechnologyInventors: Joel L. Pomerantz, Phillip A. Sharp, Carl O. Pabo
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Patent number: 7393318Abstract: The present invention provides methods and compositions for interaction trap assays for detecting protein-protein, protein-DNA, or protein-RNA interactions. The methods and compositions of the invention may also be used to identify agents which may agonize or antagonize a protein-protein, protein-DNA, or protein-RNA interaction. In certain embodiments, the interaction trap system of the invention is useful for screening libraries with greater than 107 members. In other embodiments, the interaction trap system of the invention is used in conjunction with flow cytometry. The invention further provides a means for simultaneously screening a target protein or nucleic acid sequence for the ability to interact with two or more test proteins or nucleic acids.Type: GrantFiled: March 16, 2004Date of Patent: July 1, 2008Assignee: Massachusetts Institute of TechnologyInventors: J. Keith Joung, Jeffrey Miller, Carl O. Pabo
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Patent number: 7297491Abstract: The present invention provides methods and compositions for interaction trap assays for detecting protein-protein, protein-DNA, or protein-RNA interactions. The methods and compositions of the invention may also be used to identify agents which may agonize or antagonize a protein-protein, protein-DNA, or protein-RNA interactions. In certain embodiments, the interaction trap system of the invention is useful for screening libraries with greater than 107 members. In other embodiments, the interaction trap system of the invention is used in conjunction with flow cytometry. The invention further provides a means for simultaneously screening a target protein or nucleic acid sequence for the ability to interact with two or more test proteins or nucleic acids.Type: GrantFiled: August 10, 2004Date of Patent: November 20, 2007Assignee: Massachusetts Institute of TechnologyInventors: J. Keith Joung, Jeffrey Miller, Carl O. Pabo
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Patent number: 7153949Abstract: Polynucleotides encoding chimeric proteins, and methods for their production and use are disclosed. The chimeric proteins comprise a flexible linker between two zinc finger DNA-binding domains, wherein the linker contains eight or more amino acids between the second conserved histidine residue of the carboxy-terminal zinc finger of the first domain and the first conserved cysteine residue of the amino-terminal zinc finger of the second domain.Type: GrantFiled: April 20, 2005Date of Patent: December 26, 2006Assignee: Massachusetts Institute of TechnologyInventors: Jin-Soo Kim, Carl O. Pabo
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Patent number: 7029847Abstract: The present invention provides methods and compositions for interaction trap assays for detecting protein-protein, protein-DNA, or protein-RNA interactions. The methods and compositions of the invention may also be used to identify agents which may agonize or antagonize a protein-protein, protein-DNA, or protein-RNA interaction. In certain embodiments, the interaction trap system of the invention is useful for screening libraries with greater than 107 members. In other embodiments, the interaction trap system of the invention is used in conjunction with flow cytometry. The invention further provides a means for simultaneously screening a target protein or nucleic acid sequence for the ability to interact with two or more test proteins or nucleic acids.Type: GrantFiled: November 14, 2001Date of Patent: April 18, 2006Assignee: Massachusetts Institute of TechnologyInventors: J. Keith Joung, Jeffrey Miller, Carl O. Pabo
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Patent number: 7008780Abstract: Chimeric proteins containing composite DNA-binding regions are disclosed together with DNA constructs encoding them, compositions containing them and applications in which they are useful.Type: GrantFiled: May 10, 2001Date of Patent: March 7, 2006Assignee: Massachusetts Institute of TechnologyInventors: Joel L. Pomerantz, Phillip A. Sharp, Carl O. Pabo
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Patent number: 6903185Abstract: Chimeric proteins, and methods for their production and use are disclosed. The chimeric proteins comprise a flexible linker between two zinc finger DNA-binding domains, wherein the linker comprises eight or more amino acids between the second conserved histidine residue of the carboxy-terminal zinc finger of the first domain and the first conserved cysteine residue of the amino-terminal zinc finger of the second domain.Type: GrantFiled: May 13, 2002Date of Patent: June 7, 2005Assignee: Massachusetts Institute of TechnologyInventors: Jin-Soo Kim, Carl O. Pabo
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Publication number: 20040146931Abstract: The present invention provides methods and compositions for interaction trap assays for detecting protein-protein, protein-DNA, or protein-RNA interactions. The methods and compositions of the invention may also be used to identify agents which may agonize or antagonize a protein-protein, protein-DNA, or protein-RNA interaction. In certain embodiments, the interaction trap system of the invention is useful for screening libraries with greater than 107 members. In other embodiments, the interaction trap system of the invention is used in conjunction with flow cytometry. The invention further provides a means for simultaneously screening a target protein or nucleic acid sequence for the ability to interact with two or more test proteins or nucleic acids.Type: ApplicationFiled: March 16, 2004Publication date: July 29, 2004Applicant: Massachusetts Institute of TechnologyInventors: J. Keith Joung, Jeffrey Miller, Carl O. Pabo
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Publication number: 20030126624Abstract: Chimeric proteins containing composite DNA-binding regions are disclosed together with DNA constructs encoding them, compositions containing them and applications in which they are useful.Type: ApplicationFiled: May 10, 2001Publication date: July 3, 2003Inventors: Joel L. Pomerantz, Phillip A. Sharp, Carl O. Pabo
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Publication number: 20030044787Abstract: The present invention provides methods and compositions for interaction trap assays for detecting protein-protein, protein-DNA, or protein-RNA interactions. The methods and compositions of the invention may also be used to identify agents which may agonize or antagonize a protein-protein, protein-DNA, or protein-RNA interaction. In certain embodiments, the interaction trap system of the invention is useful for screening libraries with greater than 107 members. In other embodiments, the interaction trap system of the invention is used in conjunction with flow cytometry. The invention further provides a means for simultaneously screening a target protein or nucleic acid sequence for the ability to interact with two or more test proteins or nucleic acids.Type: ApplicationFiled: May 16, 2001Publication date: March 6, 2003Inventors: J. Keith Joung, Jeffrey Miller, Carl O. Pabo