Abstract: Methods, devices, and kits are provided for performing PCR in <20 seconds per cycle, with improved efficiency and yield.

Abstract: Methods, devices, and kits are provided for performing PCR in <20 seconds per cycle, with improved efficiency and yield.

Abstract: The present disclosure is directed to methods and processes that may be used to increase the signal of a target-specific reporter molecule (such as a probe) by covalently attaching plural copies of a fluorophore to a target-reporter duplex that are excited by iFRET (induced fluorescence resonance energy transfer) from donor fluorescence of a double-stranded DNA-binding dye bound to the double-stranded DNA structure created by hybridization of reporter and target during an amplification reaction. In one illustrative example, a double-stranded DNA-binding dye is provided in solution and during amplification and/or after completion of amplification, the dye binds to the probe-target duplex, and provides fluorescence resonance energy transfer to multiple acceptor fluorophores that are covalently attached to the duplex.

Abstract: A method and system have been provided to perform high speed nucleic acid melting analysis while still obtaining accurate melting curve sufficient for genotyping. This rapid ability to interrogate DNA should be useful whenever time to result is important, such as in molecular point of care testing. Specifically, microfluidics enables genotyping by melting analysis at rates up to 50° C./s, requiring less than is to acquire an entire melting curve. High speed melting reduces the time for melting analysis, decreases errors, and improves genotype discrimination of small amplicons.

Abstract: Methods are provided for nucleic acid analysis wherein a target nucleic acid is mixed with a dsDNA binding dye to form a mixture. Optionally, an unlabeled probe is included in the mixture. A melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.

Abstract: Methods, devices, and kits are provided for performing PCR in <20 seconds per cycle, with improved efficiency and yield.

Abstract: A method of background removal from melting curves generated using a fluorescent dye is provided for analyzing a melting profile of a nucleic acid sample. The method comprises measuring the fluorescence of the nucleic acid sample as a function of temperature to produce a raw melting curve having a melting transition, the nucleic acid sample comprising a nucleic acid and a molecule that binds the nucleic acid to form a fluorescently detectable complex, the raw melting curve comprising a background fluorescence signal and a nucleic acid sample signal; and separating the background signal from the nucleic acid sample signal by use of a quantum algorithm to generate a corrected melting curve, the corrected melting curve comprising the nucleic acid sample signal.

Abstract: Methods, devices, and kits are provided for performing PCR and other thermal cycling reactions in <20 seconds per cycle, using induction heating.

Abstract: Methods, devices, and kits are provided for performing PCR in <20 seconds per cycle, with improved efficiency and yield.

Abstract: Methods, kits and mixtures are provided for performing RT-PCR with an RT incubation of no more than one minute and PCR cycles in <20 seconds per cycle.

Abstract: Methods are provided for nucleic acid analysis wherein a target nucleic acid is mixed with a dsDNA binding dye to form a mixture. Optionally, an unlabeled probe is included in the mixture. A melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.

Abstract: Methods are provided for nucleic acid analysis wherein a target nucleic acid is mixed with a dsDNA binding dye to form a mixture. Optionally, an unlabeled probe is included in the mixture. A melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.

Abstract: Methods, devices, and kits are provided for performing PCR in <20 seconds per cycle, with improved efficiency and yield.

Abstract: Methods, kits and mixtures are provided for performing RT-PCR with an RT incubation of no more than one minute and PCR cycles in <20 seconds per cycle.

Abstract: Devices are provided for performing polymerase chain reaction (PCR).

Abstract: Methods, devices, and kits are provided for performing PCR in <20 seconds per cycle, with improved efficiency and yield.

Abstract: A method and system have been provided to perform high speed nucleic acid melting analysis while still obtaining accurate melting curve sufficient for genotyping. This rapid ability to interrogate DNA should be useful whenever time to result is important, such as in molecular point of care testing. Specifically, microfluidics enables genotyping by melting analysis at rates up to 50° C./s, requiring less than is to acquire an entire melting curve. High speed melting reduces the time for melting analysis, decreases errors, and improves genotype discrimination of small amplicons.

Abstract: Methods are provided for fast nucleic acid amplification in which concentrations of primers and polymerase are increased.

Abstract: Methods are provided for nucleic acid analysis wherein a target nucleic acid that is at least partially double stranded is mixed with a dsDNA binding dye having a percent saturation of at least 50% to form a mixture. In one embodiment, the nucleic acid is amplified in the presence of the dsDNA binding dye, and in another embodiment a melting curve is generated for the target nucleic acid by measuring fluorescence from the dsDNA binding dye as the mixture is heated. Dyes for use in nucleic acid analysis and methods for making dyes are also provided.

Abstract: Systems, methods, and kits are provided wherein a temperature-sensitive reagent that emits a luminescent signal is used to adjust the identification of the temperature of a sample or to control thermocycling. In various illustrative embodiments, the sample is a PCR mixture.