Abstract: The present invention comprises methods and systems that use acoustic radiation pressure.

Abstract: A method to produce an assay is provided. The method includes introducing into a channel having a peripheral wall a first fluid having a first acoustic contrast, and introducing into a substantially peripheral portion of the channel a second fluid having a second acoustic contrast that is different from the first acoustic contrast, the substantially peripheral portion reaching from within the channel to the peripheral wall of the channel, wherein the second fluid is a biological fluid. The method further includes applying acoustic radiation pressure to the channel, and acoustically reorienting the second fluid based upon the difference between the first acoustic contrast and the second acoustic contrast such that the acoustically reoriented second fluid is translated from the substantially peripheral portion of the channel to a substantially internal portion of the channel that does not reach the peripheral wall of the channel.

Abstract: The present invention comprises methods and systems that use acoustic radiation pressure.

Abstract: A method for acoustically reorienting a fluid in a channel includes introducing into a channel a first fluid having a first acoustic contrast, introducing into the channel a second fluid having a second acoustic contrast that is different from the first acoustic contrast, applying acoustic radiation pressure to the channel, and acoustically reorienting the second fluid based upon the difference between the first acoustic contrast and the second acoustic contrast.

Abstract: A method of focusing particles is provided. The method includes transiting a fluid containing particles therein through a channel at a flow rate and adjusting the flow rate for a desired transit time through an interrogation zone through which a light from an excitation source passes. The method further includes optically exciting the particles with the excitation source, detecting an optical signal from the particles, and analyzing the optical signal. The particles may be droplets. Further, the particles may transit the interrogation zone in single file. A system of focusing particles is also provided. The system includes a channel having an inlet for accepting a fluid containing particles. The system further includes a flow adjuster configured to adjust the flow rate for a desired transit time through an interrogation zone, a light source configured to optically excite the particles, and a detector configured to detect optical signals from the particles.

Abstract: A method for imaging acoustically manipulated particles includes introducing a fluid containing a population of particles therein to a flow cell; applying acoustic radiation pressure to the flow cell; acoustically focusing the population of particles within the flow cell to concentrate the population of particles; interrogating some of the concentrated population of particles with an interrogation source to obtain an optical signal from some of the population of particles to yield population statistical data; and constructing a sampling matrix of particles from subpopulations of the concentrated population of particles to produce a high content image representative of the population of particles.

Abstract: The present invention comprises methods and systems that use acoustic radiation pressure.

Abstract: The present invention comprises methods and systems that use acoustic radiation pressure.

Abstract: The present invention comprises methods and systems that use acoustic radiation pressure.

Abstract: The present invention provides a method for screening of a large number of compounds for their ability to modulate the expression of genes. The method uses gene trap technology and comprises the steps of transfecting a population of cells with a gene-trap vector, sorting cells according to their level of fluorescence, distributing sorted cells into pools and expanding the pools to obtain a sufficient number of cells representing each trapped gene to permit distinction of the effect of a test compound over controls, exposing the cells to the test compounds and identifying compounds which alter the fluorescence distribution pattern of cells using FACS analysis.

Abstract: The present invention provides a method for screening of a large number of compounds for their ability to modulate the expression of genes. The method uses gene trap technology and comprises the steps of transfecting a population of cells with a gene-trap vector, sorting cells according to their level of fluorescence, distributing sorted cells into pools and expanding the pools to obtain a sufficient number of cells representing each trapped gene to permit distinction of the effect of a test compound over controls, exposing the cells to the test compounds and identifying compounds which alter the fluorescence distribution pattern of cells using FACS analysis.

Abstract: An electrostatic deceleration system is provided for use with a flow cytometer to decelerate the movement of electrically charged droplets formed by the flow cytometer in order to minimize damage to the cells contained in the droplets when the droplets are collected in corresponding collection vessels. The deceleration system includes a plurality of deceleration devices which are each used to decelerate droplets charged to a particular magnitude and polarity. Each deceleration device includes a steering plate arrangement and a deceleration ring arrangement. The steering plate arrangement directs the droplets having a particular magnitude and polarity of charge into the corresponding deceleration ring arrangement. The deceleration ring arrangement includes a plurality of deceleration rings to which are applied electrical potentials of a polarity the same as the polarity of the charged droplet.

Abstract: A method is provided for the rapid base sequencing of DNA or RNA fragments wherein a single fragment of DNA or RNA is provided with identifiable bases and suspended in a moving flow stream. An exonuclease sequentially cleaves individual bases from the end of the suspended fragment. The moving flow stream maintains the cleaved bases in an orderly train for subsequent detection and identification. In a particular embodiment, individual bases forming the DNA or RNA fragments are individually tagged with a characteristic fluorescent dye. The train of bases is then excited to fluorescence with an output spectrum characteristic of the individual bases. Accordingly, the base sequence of the original DNA or RNA fragment can be reconstructed.