Abstract: This invention relates to oral care compositions comprising guanidine, zinc citrate and zinc oxide, and a fluoride source, as well as to methods of using and of making these compositions.

Abstract: A device for testing aqueous laboratory samples in one embodiment includes a base member extending along a longitudinal axis and defining a plurality of sample chambers recessed in a first outer surface. A resiliently deformable first gasket is disposed on the first outer surface and comprises openings each corresponding to a respective one of the chambers. At least a first cover glass is disposed on the first gasket to enclose the openings. A peripheral clamping frame disposed on the first cover glass comprises resiliently flexible cantilevered locking protrusions configured to form a snap interlock with the base member and compress the first cover glass between the clamping frame and first gasket to seal the chambers. Some embodiments include a second resiliently deformable gasket compressed between the clamping frame and first cover glass. Fluid couplings associated with each chamber allow fluid to enter and exit the chambers.

Abstract: The disclosure relates to methods of enhancing beneficial oral bacteria and decreasing harmful oral bacteria comprising administering oral care compositions comprising a saccharide prebiotic, e.g., selected from hemolysin, N-acetyl-D-glucosamine, ?-D-lactose, D-(+)-trehalose or D-(+)-raffinose and mixtures thereof; and oral care compositions for use in such methods. The disclosure also relates to methods of using prebiotic oral care compositions, methods of screening, and methods of manufacture.

Abstract: Described herein are methods and systems for identifying a composition having anti-pathogenic activity; evaluating the anti-pathogenic activity of a composition; measuring the metabolic function of a pathogen; and/or comparing the anti-pathogenic performance of a plurality of compositions.

Abstract: The disclosure relates to oral care compositions comprising guanidine in free or orally acceptable salt form, a cationic quaternary ammonium compound (e.g., a pyridinium compound) (e.g., cetyl pyridinium chloride (CPC)), a fluoride source, and optionally a zinc source (e.g., zinc lactate or zinc citrate and zinc oxide) as well as to methods of using and of making these compositions.

Abstract: The disclosure relates to oral care compositions comprising guanidine in free or orally acceptable salt form and a stannous ion source (e.g., stannous fluoride) as well as to methods of using and of making these compositions.

Abstract: Described herein are methods of treating biofilm (e.g. modifying surface topography, reducing thickness or reducing biovolume) and compositions and apparatus for use in carrying out such methods.

Abstract: A system for testing biological samples includes a frame having first and second sides and an aperture extending through the frame from the first side to the second side. First and second covers are attached to the first and second sides of the frame to form a well bounded by the frame, the first cover, and the second cover. An electromagnetic imaging device is used to image the biological sample through the first cover. A method is also conceived, wherein a first fluid is supplied to the well, the first fluid including live cells. A biofilm is grown from the live cells. A second fluid is supplied to the well and the biofilm is imaged using the electromagnetic imaging device.

Abstract: Antimicrobial compositions and methods for depositing or coating the antimicrobial or antibacterial compositions on a substrate to prevent microbial adhesion are provided. The antimicrobial composition may include a cationic polymer having a poly-allylamine backbone. A portion of the poly-allylamine backbone may be functionalized with at least one of a guanidine functional group and a biguanide functional group.

Abstract: The disclosure relates to methods of enhancing beneficial oral bacteria and decreasing harmful oral bacteria comprising administering oral care compositions comprising a saccharide prebiotic, e.g., selected from D-mannose, N-acetyl-D-mannosamine and mixtures thereof; and oral care compositions for use in such methods. The disclosure also relates to methods of using prebiotic oral care compositions, methods of screening, and methods of manufacture.

Abstract: An oral biology model which comprises biofilm, oral epithelial tissue and a suspension of neutrophil-like cells in media is disclosed. The biofilm, which comprises oral bacteria, is produced by culturing oral bacteria on a solid substrate. The oral epithelial tissue may be gingival epithelial tissue to model the gingival crevice or buccal epithelial tissue to model the oral check. The suspension of neutrophil-like cells in media comprises neutrophil-like cells that are differentiated HL60 cells induced to a neutrophil-like phenotype by treatment with retinoic acid. Methods of using the oral biology model to test and compare compounds and formulations or to screen compounds and formulations for their effect on release of inflammatory signals, their effect on biofilm and oral bacteria and/or their effect on the cellular components are disclosed.

Abstract: Methods of increasing proteins that reduce signals associated with neutrophil recruitment and reducing proteins that induce neutrophil recruitment within an individuals gingival crevice are disclosed. The methods comprise applying to the individual's oral cavity in an amount effective to increase proteins that reduce signals associated with neutrophil recruitment and reduce proteins that induce neutrophil recruitment within an individuals gingival crevice, an oral care composition comprising: zinc oxide, zinc citrate, stannous fluoride, and optionally arginine and/or zingerone.

Abstract: Methods of increasing proteins that reduce signals associated with neutrophil recruitment and reducing proteins that induce neutrophil recruitment within an individuals gingival crevice are disclosed. The methods comprise applying to the individuals oral cavity in an amount effective to increase proteins that reduce signals associated with neutrophil recruitment and reduce proteins that induce neutrophil recruitment within an individuals gingival crevice, an oral care composition comprising: zinc phosphate, stannous fluoride and optionally, an organic acid buffer system.

Abstract: Methods of shifting biofilm composition in an individuals oral cavity are disclosed. The methods comprise applying to the individuals oral cavity an oral care composition in an amount effective to shift biofilm composition to increase the amount from healthy bacteria in biofilm relative to pathogenic bacteria in biofilm. The oral care composition comprising: zinc oxide, zinc citrate, and arginine. The shift in biofilm composition provides a balance having a greater amount of healthy bacteria in biofilm compared to pathogenic bacteria.

Abstract: Methods of reducing total pathogenic oral bacteria load within an individual's oral cavity are disclosed. Methods of selectively promoting growth of beneficial oral bacteria relative to growth of pathogenic oral bacteria in a population of bacteria that comprises beneficial oral bacteria and pathogenic oral bacteria, such as in an individual's oral cavity, are disclosed.

Abstract: This invention relates to oral care compositions comprising guanidine, zinc citrate and zinc oxide, and a fluoride source, as well as to methods of using and of making these compositions.

Abstract: An ultrasonic system may include: an oral care device including: a head; a plurality of teeth cleaning elements extending from a first side of the head; and an ultrasound module including an ultrasound transceiver positioned in the head, the ultrasound transceiver configured to produce an ultrasound signal on the first side of the head and to generate a detection signal from a reflected ultrasound signal; and a processing module operably coupled to the ultrasound module to receive the detection signal; wherein the processing module includes a programmable processor configured to process the detection signal non-linearly with respect to time by first identifying a first reflection representing a surface of a tooth and then determining whether a second reflection peak is present in the detection signal at a time prior to the first reflection peak, the second reflection peak representing a biofilm on the surface of the tooth.

Abstract: Methods of selectively inhibiting co-aggregation of commensal and pathogenic bacteria in a population of bacteria that comprises commensal and pathogenic bacteria, such as in an individual's oral cavity are disclosed. Methods of reducing biofilm in an individual's oral cavity are disclosed. Methods of inhibiting biofilm formation in a population of bacteria that comprises commensal and pathogenic bacteria are disclosed. Methods of identifying a compound or composition that enhances the selective inhibition of coaggregation of commensal and pathogenic bacteria by arginine or an arginine oligomer are also disclosed. Methods of identifying a compound or composition that enhances the selective inhibition of biofilm formation of commensal and pathogenic bacteria by arginine or an arginine oligomer are disclosed.

Abstract: Disclosed herein is mucin-coated silica, compositions comprising the same, and methods of promoting the aggregation and/or clearance of oral bacteria using such compositions.

Abstract: The present disclosure relates to oral care compositions providing oral and/or systemic benefits and/or composed to facilitate recovery following oral surgery. In some embodiments, the oral care compositions of the present disclosure comprise one or more zinc ion sources (e.g., zinc oxide and zinc citrate) and a stannous ion source (e.g., stannous fluoride), and optionally an amino acid (e.g., arginine or a salt thereof), as well as to methods of making these compositions.