Abstract: The invention relates to a devices and methods for detecting the presence of one or more analytes in an interfering fraction-containing fluid or semi-fluid sample, using an adsorbent medium comprising at least two discrete superposed layers being a first set of one or more clean-up layers and a second set of one or more detection layers through which at least a part of the sample is able to be transported in said order, characterized in that the first set of layers comprises an adsorbent medium capable of adsorbing at least a part of the interfering fraction of the sample and the second set of layers comprises an adsorbent medium containing one or more analyte-receptors capable of retaining the one or more analytes.

Abstract: The invention relates to a device for detecting the presence of an analyte in an interfering fraction containing fluid or semi-fluid sample, said device comprises:(a) a transparent housing,(b) inlet means for the sample to be analyzed,(c) outlet means, and (d) at least two discrete superposed layers being a first and a second layer through which at least a part of the sample is able to be transported in said order, characterized in that the first layer comprises an adsorbent medium capable of adsorbing at least a part of the interfering fraction of the sample and the second layer comprises an adsorbent medium containing an analyte-receptor capable of retaining the analyte. The invention further relates to the use of said device for detecting the presence of an analyte in an interfering fraction containing a fluid or semi-fluid sample under investigation and to a method for detecting the presence of an analyte in an interfering fraction containing a fluid or semi-fluid sample.