Patents by Inventor Carmelita G. Frondoza
Carmelita G. Frondoza has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 6886568Abstract: Disclosed is a method of growing cells on biodegradable microcarrier particles and more specifically growing chondrocytes for an extended period of time until they aggregate. These aggregated cells can be injected directly or shaped for implantation into the body. In another embodiment of this invention, the cell microcarrier aggregates are grown in a mold that is shaped to conform to the geometry of the desired body part to be replaced. An apparatus for shaping the aggregated cells is disclosed. The aggregated cells can be supplied in a kit.Type: GrantFiled: April 4, 2001Date of Patent: May 3, 2005Assignees: The Johns Hopkins University, Chrondros, Inc.Inventors: Carmelita G. Frondoza, David J. Fink, David S. Hungerford, Alan H. Shikani
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Publication number: 20040117033Abstract: This invention is a method for the implantation of a combination of cells or cell-microcarrier aggregates wherein one component comprises a solid implantable construct and a second component comprises an injectable formulation. For example, in one embodiment, the solid implant may be first implanted to fill the majority of the cavity receiving the implant, and then cells or cell-microcarrier aggregates in an injectable format, with or without the addition of gelling materials to promote rapid gelling in situ, may be injected into spaces surrounding the solid implant in order to secure the solid implant in the site and/or to promote rapid adherence and/or integration of the solid implant to surrounding tissues. Also contemplated in this embodiment is that the cellular composition of the injectable component may differ from that of the solid component.Type: ApplicationFiled: December 9, 2003Publication date: June 17, 2004Inventors: Carmelita G. Frondoza, David S. Hungerford, Alan H. Shikani, Abraham J. Domb, David J. Fink, Leonard Bloom
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Publication number: 20040044408Abstract: The invention is directed to the culture of cells, and particularly chondrocytes for purpose of tissue replacement. The cells are cultured on polymer constructs. Integren expression is used as a measure of chondrocyte viability. Chondrocytes are obtained from the knee, nose and ankle cartilage. Mechanical strain is used to propagate chondrocytes, chitosan and arabinogalactan-chitosan are used as scaffolds. Progenitor, pluripotential, stem and mesenchymal cells are operative in this invention.Type: ApplicationFiled: September 3, 2003Publication date: March 4, 2004Inventors: David S. Hungerford, Carmelita G. Frondoza, Alan H. Shikani, Abraham J. Domb
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Patent number: 6662805Abstract: This invention is a method for the implantation of a combination of cells or cell-microcarrier aggregates wherein one component comprises a solid implantable construct and a second component comprises an injectable formulation. For example, in one embodiment, the solid implant may be first implanted to fill the majority of the cavity receiving the implant, and then cells or cell-microcarrier aggregates in an injectable format, with or without the addition of gelling materials to promote rapid gelling in situ, may be injected into spaces surrounding the solid implant in order to secure the solid implant in the site and/or to promote rapid adherence and/or integration of the solid implant to surrounding tissues. Also contemplated in this embodiment is that the cellular composition of the injectable component may differ from that of the solid component.Type: GrantFiled: August 6, 2001Date of Patent: December 16, 2003Assignees: The Johns Hopkins University, Chondros, Inc.Inventors: Carmelita G. Frondoza, David S. Hungerford, Alan H. Shikani, Abraham J. Domb, David J. Fink, Leonard Bloom
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Patent number: 6637437Abstract: The invention is directed to the culture of cells, and particularly chondrocytes for purpose of tissue replacement. The cells are cultured on polymer constructs. Integren expression is used as a measure of chondrocyte viability. Chondrocytes are obtained from the knee, nose and ankle cartilage. Mechanical strain is used to propagate chondrocytes, chitosan and arabinogalactanchitosan are used as scaffolds. Progenitor, pluripotential, stem and mesenchymal cells are operative in this invention.Type: GrantFiled: November 14, 2000Date of Patent: October 28, 2003Assignees: Johns Hopkins University, Chondros, Inc.Inventors: David S. Hungerford, Carmelita G. Frondoza, Alan H. Shikani, Abraham J. Domb
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Publication number: 20020133235Abstract: Cells grown on a microcarrier are separated from the microcarrier by enzymatically digesting the microcarrier. More specifically, chondrocytes may be grown on dextran microcarrier beadlets and then the beadlets digested using dextranase to separate the chondrocytes from the carrier. Cells can also be grown on chitosan microcarriers to be used for implantation. In addition, cells can be grown on polysaccharide polymers to be used as implant devices. Various polymers serve as scaffolds for cells to be used for implantation. The polymers can be used for cell culture as well as for preparing scaffolds useful for tissue replacement such as cartilage tissue.Type: ApplicationFiled: February 4, 2002Publication date: September 19, 2002Inventors: David S. Hungerford, Carmelita G. Frondoza, Afshin Sohrobi, Alan H. Shikani, Abraham J. Domb
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Publication number: 20020123142Abstract: Cells grown on a microcarrier are separated from the microcarrier by enzymatically digesting the microcarrier. More specifically, chondrocytes may be grown on dextran microcarrier beadlets and then the beadlets digested using dextranase to separate the chondrocytes from the carrier. Cells can also be grown on chitosan microcarriers to be used for implantation. In addition, cells can be grown on polysaccharide polymers to be used as implant devices. Various polymers serve as scaffolds for cells to be used for implantation. The polymers can be used for cell culture as well as for preparing scaffolds useful for tissue replacement such as cartilage tissue.Type: ApplicationFiled: January 3, 2002Publication date: September 5, 2002Inventors: David S. Hungerford, Carmelita G. Frondoza, Afshin Sohrabi, Alan H. Shikani, Abraham J. Domb
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Patent number: 6378527Abstract: Cells grown on a microcarrier are separated from the microcarrier by enzymatically digesting the microcarrier. More specifically, chondrocytes may be grown on dextran microcarrier beadlets and then the beadlets digested using dextranase to separate the chondrocytes from the carrier. Cells can also be grown on chitosan microcarriers to be used for implantation. In addition, cells can be grown on polysaccharide polymers to be used as implant devices. Various polymers serve as scaffolds for cells to be used for implantation. The polymers can be used for cell culture as well as for preparing scaffolds useful for tissue replacement such as cartilage tissue.Type: GrantFiled: March 24, 1999Date of Patent: April 30, 2002Assignee: Chondros, Inc.Inventors: David S. Hungerford, Carmelita G. Frondoza, Afshin Sohrabi, Alan H. Shikani, Abraham J. Domb
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Publication number: 20010051834Abstract: This invention is a method for the implantation of a combination of cells or cell-microcarrier aggregates wherein one component comprises a solid implantable construct and a second component comprises an injectable formulation. For example, in one embodiment, the solid implant may be first implanted to fill the majority of the cavity receiving the implant, and then cells or cell-microcarrier aggregates in an injectable format, with or without the addition of gelling materials to promote rapid gelling in situ, may be injected into spaces surrounding the solid implant in order to secure the solid implant in the site and/or to promote rapid adherence and/or integration of the solid implant to surrounding tissues. Also contemplated in this embodiment is that the cellular composition of the injectable component may differ from that of the solid component.Type: ApplicationFiled: August 6, 2001Publication date: December 13, 2001Applicant: Chondros, Inc.Inventors: Carmelita G. Frondoza, David S. Hungerford, Alan H. Shikani, Abraham J. Domb, David J. Fink, Leonard Bloom
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Publication number: 20010014475Abstract: Disclosed is a method of growing cells on biodegradable microcarrier particles and more specifically growing chondrocytes for an extended period of time until they aggregate. These aggregated cells can be injected directly or shaped for implantation into the body. In another embodiment of this invention, the cell microcarrier aggregates are grown in a mold that is shaped to conform to the geometry of the desired body part to be replaced. An apparatus for shaping the aggregated cells is disclosed. The aggregated cells can be supplied in a kit.Type: ApplicationFiled: April 4, 2001Publication date: August 16, 2001Inventors: Carmelita G. Frondoza, David J. Fink, David S. Hungerford, Alan H. Shikani