Abstract: The invention relates to a dual-recognition immunoassay for the detection of antibodies specific to a target antigen in a sample which comprises contacting said target antigen with a sample suspected of containing said antibodies specific to said target antigen under conditions allowing the formation of an antigen-antibody complex; adding a conjugate comprising said target antigen and a marker under conditions allowing the formation of an antigen-antibody-antigen/marker complex; and detecting said antigen-antibody-antigen/marker complex. The immunoassay can be used, among other applications, in the diagnosis of infections caused by pathogenic organisms with high sensitivity and specificity.

Abstract: The present invention is a method for the production of a subunit vaccine against canine parvovirus (CPV). The method includes the step wherein a recombinant protein VP2 of CPV is obtained by using the replication of a recombinant baculovirus wherein the gene corresponding to VP2 has been previously inserted in cells of a permissive host. The protein VP2 obtained has the capacity of forming empty VP2 capsids with high immunogenicity and can be provided as a vaccine formulation for protecting dogs against CPV infection the recombinant baculovirus AcMNPV.pCPVEx17 expresses the VP2 of CPV in conditions making possible the formulation of pseudo-viral capsids.

Abstract: An immunogenic peptide for use in the prevention of parvovirus infections having a contiguous sequence of 6 to 25 amino acids. The peptide is produced from autonomous parvoviruses so as to be capable of inducing neutralizing antibodies against such viruses. These peptides correspond to antigenic sites located in the first 25 amino acids of the amino terminal end of the VP2 proteins of parvoviruses. When these peptides are coupled to carrier proteins or to other immunogenic complexes, they can be used in the formulation of vaccines appropriate to protect dogs, cats, pigs and minks against the infections caused by the parvoviruses.

Abstract: Method for the production of a subunit vaccine against porcine parvovirus (PPV). The method is comprised of a first step wherein a recombinant protein VP2 of PPV is obtained by using the replication of a recombinant baculovirus wherein the gene corresponding to VP2 has been previously inserted in cells of a permissive host. The protein VP2 obtained in this invention has the capacity of forming empty chimeric capsids with high immunogenicity and can be provided as a vaccine formulation for protecting pigs against PPV infection. The recombinant baculovirus AcMNPV.pPPVEx8 expresses the VP2 of PPV in conditions making possible the formation of pseudo-viral capsids.