Patents by Inventor Carol A. Kreader

Carol A. Kreader has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20220315972
    Abstract: Methods for primer switching during amplification reactions are provided. In particular, methods are provided for converting single primer PCR amplicons to dual primer PCR amplicons.
    Type: Application
    Filed: June 10, 2022
    Publication date: October 6, 2022
    Inventors: Brian W. Ward, Carol A. Kreader, Jaime K. Robert, Kenneth E. Heuermann
  • Patent number: 11390913
    Abstract: Methods for primer switching during amplification reactions are provided. In particular, methods are provided for converting single primer PCR amplicons to dual primer PCR amplicons.
    Type: Grant
    Filed: September 22, 2017
    Date of Patent: July 19, 2022
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: Brian W. Ward, Carol A. Kreader, Jaime K. Robert, Kenneth E. Heuermann
  • Patent number: 11268144
    Abstract: The present disclosure provides reagents and methods for molecular proximity detection of specific endogenous nucleic acids in situ using RNA-guided nucleic acid binding proteins.
    Type: Grant
    Filed: September 23, 2016
    Date of Patent: March 8, 2022
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: Gregory D. Davis, Vikas B. Palhan, Carol A. Kreader
  • Patent number: 10711266
    Abstract: The present disclosure provides methods and kits for isolating miRNAs from biological fluids.
    Type: Grant
    Filed: October 15, 2018
    Date of Patent: July 14, 2020
    Assignee: Sigma-Aldrich Co. LLC
    Inventor: Carol A. Kreader
  • Patent number: 10711296
    Abstract: Processes, oligonucleotides, and kits for amplifying RNA. In particular, the processes generate and amplify cDNA libraries in which the orientation of the input RNA molecule is preserved in the products. Among the various aspects of the present disclosure is the provision of process for directionally amplifying RNA. The process comprises reverse transcribing at least one RNA molecule in the presence of a plurality of first synthesis primers to generate a plurality of first strands of complementary DNA (cDNA), wherein each of the first synthesis primers comprises a 3? sequence having complementarity to a portion of the RNA molecule, a non-complementary 5? sequence corresponding to one or more amplification primers, and optionally an internal tag sequence comprising a first tag sequence.
    Type: Grant
    Filed: March 24, 2016
    Date of Patent: July 14, 2020
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: Kenneth B Heuermann, Carol A Kreader, Jaime K Robert, Brian W Ward
  • Publication number: 20190211386
    Abstract: Methods for primer switching during amplification reactions are provided. In particular, methods are provided for converting single primer PCR amplicons to dual primer PCR amplicons.
    Type: Application
    Filed: September 22, 2017
    Publication date: July 11, 2019
    Inventors: Brian W. Ward, Carol A. Kreader, Jaime K. Robert, Kenneth E. Heuermann
  • Publication number: 20190144850
    Abstract: The present disclosure provides methods and kits for isolating miRNAs from biological fluids.
    Type: Application
    Filed: October 15, 2018
    Publication date: May 16, 2019
    Applicant: SIGMA-ALDRICH CO. LLC
    Inventor: Carol A. Kreader
  • Publication number: 20180340221
    Abstract: The present disclosure provides reagents and methods for molecular proximity detection of specific endogenous nucleic acids in situ using RNA-guided nucleic acid binding proteins.
    Type: Application
    Filed: September 23, 2016
    Publication date: November 29, 2018
    Applicant: SIGMA-ALDRICH CO. LLC
    Inventors: Gregory D Davis, Vikas B Palhan, Carol A. Kreader
  • Publication number: 20180044723
    Abstract: Processes, oligonucleotides, and kits for amplifying RNA. In particular, the processes generate and amplify cDNA libraries in which the orientation of the input RNA molecule is preserved in the products. Among the various aspects of the present disclosure is the provision of process for directionally amplifying RNA. The process comprises reverse transcribing at least one RNA molecule in the presence of a plurality of first synthesis primers to generate a plurality of first strands of complementary DNA (cDNA), wherein each of the first synthesis primers comprises a 3? sequence having complementarity to a portion of the RNA molecule, a non-complementary 5? sequence corresponding to one or more amplification primers, and optionally an internal tag sequence comprising a first tag sequence.
    Type: Application
    Filed: March 24, 2016
    Publication date: February 15, 2018
    Applicant: Sigma-Aldrich Co. LLC
    Inventors: Kenneth B Heuermann, Carol A Kreader, Jaime K Robert, Brian W Ward
  • Publication number: 20160376610
    Abstract: Fusion protein comprising a programmable DNA modification protein and a cell cycle regulated protein, and methods of using the fusion protein to modify chromosomal sequences and/or regulate gene expression in a cell cycle dependent manner.
    Type: Application
    Filed: June 24, 2016
    Publication date: December 29, 2016
    Inventors: Gregory D. Davis, Qingzhou Ji, Carol A. Kreader
  • Publication number: 20080003574
    Abstract: The present invention provides methods and compositions for extracting RNA from cells. The cellular extract may be directly used in a variety of reactions, such as reverse transcription and PCR.
    Type: Application
    Filed: June 28, 2006
    Publication date: January 3, 2008
    Applicant: Sigma-Aldrich Co.
    Inventors: Steve S. Michalik, Scott A. Weber, Carol A. Kreader
  • Publication number: 20080003575
    Abstract: The present invention provides methods and compositions for extracting RNA from cells. The cellular extract may be directly used in a variety of reactions, such as reverse transcription and PCR.
    Type: Application
    Filed: June 28, 2006
    Publication date: January 3, 2008
    Applicant: Sigma-Aldrich Co.
    Inventors: Steve S. Michalik, Scott A. Weber, Carol A. Kreader