Patents by Inventor Carol Kreader

Carol Kreader has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Targeted histone acetylation
    Patent number: 10570378
    Abstract: The present disclosure provides compositions and methods for acetylating histones at targeted chromosomal locations in a cell. In particular, the disclosure provides a fusion protein comprising a DNA binding domain and at least one histone acetyltransferase (HAT) domain, such that the DNA binding domain targets the fusion protein to a targeted chromosomal location and the HAT domain acetylates histones at the targeted location.
    Type: Grant
    Filed: February 28, 2013
    Date of Patent: February 25, 2020
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: Qingzhou Ji, Carol Kreader
  • VISUALIZING MODIFIED NUCLEOTIDES AND NUCLEIC ACID INTERACTIONS IN SINGLE CELLS
    Publication number: 20190177780
    Abstract: Methods for visualizing modified nucleotides in a specific nucleic acid sequence or specific nucleic acid sequence interactions in single cells, wherein the methods comprise coupling an in situ hybridization (ISH) reaction with a proximity ligation assay (PLA) reaction.
    Type: Application
    Filed: February 22, 2019
    Publication date: June 13, 2019
    Applicant: SIGMA-ALDRICH CO. LLC
    Inventors: Vikas Palhan, Carol Kreader
  • Visualizing modified nucleotides and nucleic acid interactions in single cells
    Patent number: 10253356
    Abstract: Methods for visualizing modified nucleotides in a specific nucleic acid sequence or specific nucleic acid sequence interactions in single cells, wherein the methods comprise coupling an in situ hybridization (ISH) reaction with a proximity ligation assay (PLA) reaction.
    Type: Grant
    Filed: December 22, 2015
    Date of Patent: April 9, 2019
    Assignee: Sigma-Aldrich Co. LLC
    Inventors: Vikas Palhan, Carol Kreader
  • Micro RNA isolation from biological fluid
    Patent number: 10131902
    Abstract: The present disclosure provides methods and kits for isolating miRNAs from biological fluids.
    Type: Grant
    Filed: November 25, 2014
    Date of Patent: November 20, 2018
    Assignee: Sigma-Aldrich Co. LLC
    Inventor: Carol Kreader
  • VISUALIZING MODIFIED NUCLEOTIDES AND NUCLEIC ACID INTERACTIONS IN SINGLE CELLS
    Publication number: 20170362647
    Abstract: Methods for visualizing modified nucleotides in a specific nucleic acid sequence or specific nucleic acid sequence interactions in single cells, wherein the methods comprise coupling an in situ hybridization (ISH) reaction with a proximity ligation as say (PLA) reaction.
    Type: Application
    Filed: December 22, 2015
    Publication date: December 21, 2017
    Applicant: SIGMA-ALDRICH CO. LLC
    Inventors: Vikas Palhan, Carol Kreader
  • Cells for chromatin immunoprecipitation and methods for making
    Patent number: 9671393
    Abstract: The present invention provides isolated, non-viable bacterial cells comprising a plurality of nucleic acid crosslinks, methods for making said cells, and methods for using said cells to isolate DNA-protein complexes. In particular, the nucleic acids of the cells are crosslinked by contacting the cells with a furocoumarin compound and ultraviolet light.
    Type: Grant
    Filed: September 2, 2011
    Date of Patent: June 6, 2017
    Assignee: SIGMA-ALDRICH CO., LLC
    Inventors: Vikas B. Palhan, Carol Kreader, Ernie Mueller
  • MICRO RNA ISOLATION FROM BIOLOGICAL FLUID
    Publication number: 20160376582
    Abstract: The present disclosure provides methods and kits for isolating miRNAs from biological fluids.
    Type: Application
    Filed: November 25, 2014
    Publication date: December 29, 2016
    Applicant: SIGMA-ALDRICH CO. LLC
    Inventor: Carol KREADER
  • Methods and compositions for the rapid isolation of small RNA molecules
    Patent number: 9062303
    Abstract: The present invention provides methods for the rapid and efficient isolation of small RNA from a biological sample. In particular, small RNA is separated and isolated from large RNA, DNA, proteins, and other macromolecules in the biological sample.
    Type: Grant
    Filed: June 22, 2010
    Date of Patent: June 23, 2015
    Assignee: SIGMA-ALDRICH CO. LLC
    Inventors: Fuqiang Chen, Carol Kreader
  • TARGETED HISTONE ACETYLATION
    Publication number: 20150079657
    Abstract: The present disclosure provides compositions and methods for acetylating histones at targeted chromosomal locations in a cell. In particular, the disclosure provides a fusion protein comprising a DNA binding domain and at least one histone acetyltransferase (HAT) domain, such that the DNA binding domain targets the fusion protein to a targeted chromosomal location and the HAT domain acetylates histones at the targeted location.
    Type: Application
    Filed: February 28, 2013
    Publication date: March 19, 2015
    Applicant: SIGMA-ALDRICH CO. LLC
    Inventors: Qingzhou Ji, Carol Kreader
  • CELLS FOR CHROMATIN IMMUNOPRECIPITATION AND METHODS FOR MAKING
    Publication number: 20130172198
    Abstract: The present invention provides isolated, non-viable bacterial cells comprising a plurality of nucleic acid crosslinks, methods for making said cells, and methods for using said cells to isolate DNA-protein complexes. In particular, the nucleic acids of the cells are crosslinked by contacting the cells with a furocoumarin compound and ultraviolet light.
    Type: Application
    Filed: September 2, 2011
    Publication date: July 4, 2013
    Applicant: SIGMA-ALDRICH CO., LLC
    Inventors: Vikas B. Palhan, Carol Kreader, Ernie Mueller
  • REAL TIME POLYMERASE CHAIN REACTION PROCESS USING A UNIVERSAL DETECTION SYSTEM
    Publication number: 20110136118
    Abstract: Provided are methods and kits for detecting amplification of a target nucleic acid during a real time quantitative polymerase chain reaction process using a universal detection system. The detection system uses an unlabeled probe that detects the amplified target nucleic acid and interacts with a universal detection module.
    Type: Application
    Filed: June 16, 2009
    Publication date: June 9, 2011
    Applicant: SIGMA-ALDRICH CO.
    Inventors: Carol Kreader, Brian Ward
  • METHODS AND COMPOSITIONS FOR THE RAPID ISOLATION OF SMALL RNA MOLECULES
    Publication number: 20100256351
    Abstract: The present invention provides methods for the rapid and efficient isolation of small RNA from a biological sample. In particular, small RNA is separated and isolated from large RNA, DNA, proteins, and other macromolecules in the biological sample.
    Type: Application
    Filed: June 22, 2010
    Publication date: October 7, 2010
    Applicant: SIGMA ALDRICH CO.
    Inventors: Fuqiang Chen, Carol Kreader
  • Compositions and Methods for Isolation of Biological Molecules
    Publication number: 20080026374
    Abstract: The present invention provides charged separation media, kits comprising the separation media, and methods of using the same to bind and optionally isolate biological molecules. The charged separation media and methods utilize a polyion non-covalently bound to the separation media to bind the desired biological molecule to the separation media.
    Type: Application
    Filed: July 31, 2006
    Publication date: January 31, 2008
    Applicant: SIGMA ALDRICH CO.
    Inventors: Fuqiang Chen, Carol Kreader
  • Compositions and Methods for Isolation of Biological Molecules
    Publication number: 20080023395
    Abstract: The present invention provides charged separation media, kits comprising the separation media, and methods of using the same to bind and optionally isolate biological molecules. The charged separation media and methods utilize a polyion non-covalently bound to the separation media to bind the desired biological molecule to the separation media.
    Type: Application
    Filed: July 31, 2006
    Publication date: January 31, 2008
    Applicant: SIGMA ALDRICH CO.
    Inventors: Fuqiang Chen, Carol Kreader
  • Compositions and Methods for Isolation of Biological Molecules
    Publication number: 20080026375
    Abstract: The present invention provides charged separation media, kits comprising the separation media, and methods of using the same to bind and optionally isolate biological molecules. The charged separation media and methods utilize a polyion non-covalently bound to the separation media to bind the desired biological molecule to the separation media.
    Type: Application
    Filed: July 31, 2006
    Publication date: January 31, 2008
    Applicant: SIGMA ALDRICH CO.
    Inventors: Fuqiang Chen, Carol Kreader
  • Methods and compositions for the rapid isolation of small RNA molecules
    Publication number: 20070202511
    Abstract: The present invention provides extraction compositions and methods for the rapid and efficient isolation of small RNA molecules from a biological sample. In particular, the extraction compositions, when contacted with a biological sample, releases the small RNA molecules from the other molecules in a biological sample, and the released small RNA molecules may then be isolated.
    Type: Application
    Filed: February 28, 2006
    Publication date: August 30, 2007
    Inventors: Fuqiang Chen, Carol Kreader
  • Compositions and Methods for Nucleic Acid Extraction from Biological Samples
    Publication number: 20070172875
    Abstract: Methods and compositions for extracting nucleic acids from a biological sample are provided. The extraction compositions contain a protease enzyme such as proteinase K at alkaline pH with little or no surfactant present. Extraction can be efficiently performed in 60 minutes or less at room temperature for certain mammalian tissue samples and at elevated temperatures for certain plant tissues.
    Type: Application
    Filed: March 19, 2007
    Publication date: July 26, 2007
    Applicant: SIGMA-ALDRICH CO.
    Inventors: Scott Weber, Derek Douglas, Carol Kreader
  • Compositions and Methods for Nucleic Acid Extraction from Biological Samples
    Publication number: 20070172874
    Abstract: Methods and compositions for extracting nucleic acids from a biological sample are provided. The extraction compositions contain a protease enzyme such as proteinase K at alkaline pH with little or no surfactant present. Extraction can be efficiently performed in 60 minutes or less at room temperature for certain mammalian tissue samples and at elevated temperatures for certain plant tissues.
    Type: Application
    Filed: March 19, 2007
    Publication date: July 26, 2007
    Applicant: SIGMA-ALDRICH CO.
    Inventors: Scott Weber, Derek Douglas, Carol Kreader
  • Compositions and Methods for Nucleic Acid Extraction from Biological Samples
    Publication number: 20070166752
    Abstract: Methods and compositions for extracting nucleic acids from a biological sample are provided. The extraction compositions contain a protease enzyme such as proteinase K at alkaline pH with little or no surfactant present. Extraction can be efficiently performed in 60 minutes or less at room temperature for certain mammalian tissue samples and at elevated temperatures for certain plant tissues.
    Type: Application
    Filed: March 19, 2007
    Publication date: July 19, 2007
    Applicant: SIGMA-ALDRICH CO.
    Inventors: Scott Weber, Derek Douglas, Carol Kreader
  • Compositions and Methods for Nucleic Acid Extraction from Biological Samples
    Publication number: 20070166751
    Abstract: Methods and compositions for extracting nucleic acids from a biological sample are provided. The extraction compositions contain a protease enzyme such as proteinase K at alkaline pH with little or no surfactant present. Extraction can be efficiently performed in 60 minutes or less at room temperature for certain mammalian tissue samples and at elevated temperatures for certain plant tissues.
    Type: Application
    Filed: March 19, 2007
    Publication date: July 19, 2007
    Applicant: SIGMA-ALDRICH CO.
    Inventors: Scott Weber, Derek Douglas, Carol Kreader