Patents by Inventor Carol Polisson

Carol Polisson has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Type II restriction endonuclease, APO I, obtainable from arthrobacter protophormiae and a process for producing the same
    Patent number: 5200337
    Abstract: The present invention provides a novel type II restriction endonuclease obtainable from Arthrobacter protophormiae. The endonuclease known as Apo I, recognizes the following nucleotide sequence and has a cleavage point indicated by the arrows: ##STR1## Also described is a process for obtaining Apo I from Arthrobacter protophormiae.
    Type: Grant
    Filed: October 25, 1991
    Date of Patent: April 6, 1993
    Assignee: New England Biolabs, Inc.
    Inventors: Carol Polisson, Derek Robinson, Keith Lunnen
  • Type II restriction endonuclease obtainable from Kluyvera ascorbata and a process for producing the same
    Patent number: 5147794
    Abstract: The present invention provides a novel restriction endonuclease obtainable from the bacterium Kluyvera ascorbata, hereinafter referred to as "KasI", which endonuclease:(1) recognizes the base sequence in a double-stranded DNA molecule as shown below,5'--GGCGCC--3'3'--CCGCGG--5'(wherein C and G represent cytosine and guanine, respectively),(2) cleaves said sequence in th4e phosphodiester bonds between G and G as indicated with the vertical arrows; and(3) cleaves double-stranded pUC, M13mp18, and lambda DNA in one position, PhiX172, and T7 DNA in two positions, and Adeno2 DNA at 20 positions, while not cleaving SV40 DNA.The present invention further provides a recombinant DNA encoding the KasI restriction endonuclease and modification methylase obtainable from K. ascorbata, and methods for the production of the recombinant DNA encoding those enzymes. Methods for producing the KasI restriction endonuclease and modification methylase in substantially pure form are also provided.
    Type: Grant
    Filed: July 23, 1990
    Date of Patent: September 15, 1992
    Assignee: New England Biolabs, Inc.
    Inventors: Carol Polisson, Janet M. Barsomian
  • Type II restriction endonuclease obtainable from Pseudomonas alcaligenes and a process for producing the same
    Patent number: 5098839
    Abstract: The present invention provides a novel Type II restriction endonuclease obtainable from Pseudomonas alcaligenes. The endonuclease known as PacI, recognizes the following nucleotide sequence and has a cleavage point indicated by the arrows: ##STR1## Also described is a process for obtaining PacI from P. alcaligenes.
    Type: Grant
    Filed: May 10, 1990
    Date of Patent: March 24, 1992
    Assignee: New England Biolabs, Inc.
    Inventor: Carol Polisson