Patents by Inventor Carole Bornarth
Carole Bornarth has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11788126Abstract: The present disclosure is directed to compositions, methods and kits for amplifying target nucleic acids while reducing non-specific amplification and undesired amplification products using a dual hot start reaction mixture that comprise at least two different hot start mechanisms.Type: GrantFiled: August 13, 2020Date of Patent: October 17, 2023Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Carole Bornarth, Michael Lau, Junko Stevens
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Publication number: 20210323994Abstract: Method and compositions and kits for isolation, identification, and quantification of miRNAs and other small RNAs, including but not limited to, siRNAs, mRNAs, and snRNAs are disclosed. Methods of diagnosing a disease or its progression are also disclosed.Type: ApplicationFiled: June 28, 2021Publication date: October 21, 2021Inventors: Tom XU, Christopher TRINH, Carole BORNARTH, Brian EVANS, Mousumi RATH, Kathy TRAN
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Patent number: 11046727Abstract: Method and compositions and kits for isolation, identification, and quantification of miRNAs and other small RNAs, including but not limited to, siRNAs, mRNAs, and snRNAs are disclosed. Methods of diagnosing a disease or its progression are also disclosed.Type: GrantFiled: August 21, 2017Date of Patent: June 29, 2021Assignee: Life Technologies CorporationInventors: Tom Xu, Christopher Trinh, Carole Bornarth, Brian Evans, Mousumi Rath, Kathy Tran
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Publication number: 20210147946Abstract: Systems, primers, kits, and methods for detecting microsatellite instability in a biological sample are described. Signal data is received from a capillary electrophoresis genetic analysis instrument, wherein the signal data is measured from fluorescence of fragments comprising nucleic acid sequences amplified from the biological sample via polymerase chain reaction (PCR). The nucleic acid sequences correspond to a plurality of different microsatellite loci and are obtained using a plurality of PCR primers configured to flank a plurality of microsatellite loci of a biological sample. When the PCR primers and the biological sample are combined and subjected to PCR amplification, fluorescently labeled DNA fragments are generated comprising the plurality of microsatellite loci. Fluorescent data obtained from the plurality of fluorescently labelled microsatellite loci are used to classify microsatellite instability of the biological sample.Type: ApplicationFiled: November 6, 2020Publication date: May 20, 2021Inventors: Charles Wendell Higdon, III, Harrison Leong, Charles Baudo, Carole Bornarth, Edgar Schreiber
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Publication number: 20210063405Abstract: According to the present teachings, compositions, kits, and methods for protein melt analysis are provided that utilizing one or more fluorophore dyes. In some embodiments, a method comprises preparing a sample by mixing at least one protein with two or more dyes, and applying a controlled heating, while recording the fluorescence emission of the sample. The methods can be used, for example, for screening conditions for optimized protein stability, screening for ligands that bind and enhance protein stability (e.g., protein-protein interactions), screening for mutations for enhanced stability, screening crystallization conditions for protein stability, screening storage conditions for protein stability, and screening conditions in which a protein will be used (e.g., production conditions, treatment conditions, etc.) for protein stability.Type: ApplicationFiled: September 9, 2020Publication date: March 4, 2021Inventors: Carole BORNARTH, Mousumi RATH
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Patent number: 10866245Abstract: According to the present teachings, compositions, kits, and methods for protein melt analysis are provided that utilizing one or more fluorophore dyes. In some embodiments, a method comprises preparing a sample by mixing at least one protein with two or more dyes, and applying a controlled heating, while recording the fluorescence emission of the sample. The methods can be used, for example, for screening conditions for optimized protein stability, screening for ligands that bind and enhance protein stability (e.g., protein-protein interactions), screening for mutations for enhanced stability, screening crystallization conditions for protein stability, screening storage conditions for protein stability, and screening conditions in which a protein will be used (e.g., production conditions, treatment conditions, etc.) for protein stability.Type: GrantFiled: June 6, 2018Date of Patent: December 15, 2020Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Carole Bornarth, Mousumi Rath
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Publication number: 20200370109Abstract: The present disclosure is directed to compositions, methods and kits for amplifying target nucleic acids while reducing non-specific amplification and undesired amplification products using a dual hot start reaction mixture that comprise at least two different hot start mechanisms.Type: ApplicationFiled: August 13, 2020Publication date: November 26, 2020Inventors: Carole BORNARTH, Michael LAU, Junko STEVENS
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Patent number: 10767217Abstract: The present disclosure is directed to compositions, methods and kits for amplifying target nucleic acids while reducing non-specific amplification and undesired amplification products using a dual hot start reaction mixture that comprise at least two different hot start mechanisms.Type: GrantFiled: March 21, 2018Date of Patent: September 8, 2020Assignee: Life Technologies CorporationInventors: Carole Bornarth, Michael Lau, Junko Stevens
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Publication number: 20180348230Abstract: According to the present teachings, compositions, kits, and methods for protein melt analysis are provided that utilizing one or more fluorophore dyes. In some embodiments, a method comprises preparing a sample by mixing at least one protein with two or more dyes, and applying a controlled heating, while recording the fluorescence emission of the sample. The methods can be used, for example, for screening conditions for optimized protein stability, screening for ligands that bind and enhance protein stability (e.g., protein-protein interactions), screening for mutations for enhanced stability, screening crystallization conditions for protein stability, screening storage conditions for protein stability, and screening conditions in which a protein will be used (e.g., production conditions, treatment conditions, etc.) for protein stability.Type: ApplicationFiled: June 6, 2018Publication date: December 6, 2018Inventors: Carole BORNARTH, Mousumi RATH
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Publication number: 20180282797Abstract: The present disclosure is directed to compositions, methods and kits for amplifying target nucleic acids while reducing non-specific amplification and undesired amplification products using a dual hot start reaction mixture that comprise at least two different hot start mechanisms.Type: ApplicationFiled: March 21, 2018Publication date: October 4, 2018Inventors: Carole BORNARTH, Michael LAU, Junko STEVENS
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Patent number: 10024863Abstract: According to the present teachings, compositions, kits, and methods for protein melt analysis are provided that utilizing one or more fluorophore dyes. In some embodiments, a method comprises preparing a sample by mixing at least one protein with two or more dyes, and applying a controlled heating, while recording the fluorescence emission of the sample. The methods can be used, for example, for screening conditions for optimized protein stability, screening for ligands that bind and enhance protein stability (e.g., protein-protein interactions), screening for mutations for enhanced stability, screening crystallization conditions for protein stability, screening storage conditions for protein stability, and screening conditions in which a protein will be used (e.g., production conditions, treatment conditions, etc.) for protein stability.Type: GrantFiled: March 14, 2014Date of Patent: July 17, 2018Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: Carole Bornarth, Mousumi Rath
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Patent number: 9951378Abstract: The present disclosure is directed to compositions, methods and kits for amplifying target nucleic acids while reducing non-specific amplification and undesired amplification products using a dual hot start reaction mixture that comprise at least two different hot start mechanisms.Type: GrantFiled: June 14, 2013Date of Patent: April 24, 2018Assignee: Life Technologies CorporationInventors: Carole Bornarth, Michael Lau, Junko Stevens
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Publication number: 20180044371Abstract: Method and compositions and kits for isolation, identification, and quantification of miRNAs and other small RNAs, including but not limited to, siRNAs, mRNAs, and snRNAs are disclosed. Methods of diagnosing a disease or its progression are also disclosed.Type: ApplicationFiled: August 21, 2017Publication date: February 15, 2018Inventors: Tom XU, Christopher TRINH, Carole BORNARTH, Brian EVANS, Mousumi RATH, Kathy TRAN
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Publication number: 20140315190Abstract: According to the present teachings, compositions, kits, and methods for protein melt analysis are provided that utilizing one or more fluorophore dyes. In some embodiments, a method comprises preparing a sample by mixing at least one protein with two or more dyes, and applying a controlled heating, while recording the fluorescence emission of the sample. The methods can be used, for example, for screening conditions for optimized protein stability, screening for ligands that bind and enhance protein stability (e.g., protein-protein interactions), screening for mutations for enhanced stability, screening crystallization conditions for protein stability, screening storage conditions for protein stability, and screening conditions in which a protein will be used (e.g., production conditions, treatment conditions, etc.) for protein stability.Type: ApplicationFiled: March 14, 2014Publication date: October 23, 2014Inventors: Carole BORNARTH, Mousumi RATH
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Publication number: 20140099644Abstract: The present disclosure is directed to compositions, methods and kits for amplifying target nucleic acids while reducing non-specific amplification and undesired amplification products using a dual hot start reaction mixture that comprise at least two different hot start mechanisms.Type: ApplicationFiled: June 14, 2013Publication date: April 10, 2014Inventors: Carole BORNARTH, Michael LAU, Junko STEVENS
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Publication number: 20120208189Abstract: Method and compositions and kits for isolation, identification, and quantification of miRNAs and other small RNAs, including but not limited to, siRNAs, mRNAs, and snRNAs are disclosed. Methods of diagnosing a disease or its progression are also disclosed.Type: ApplicationFiled: January 13, 2012Publication date: August 16, 2012Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Tom Xu, Christopher Trinh, Carole Bornarth, Brian Evans, Mousumi Rath, Kathy Tran
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Publication number: 20080128298Abstract: Disclosed are compositions and methods for amplification of nucleic acid sequences of interest. It has been discovered that amplification reactions can produce amplification products of high quality, such as low amplification bias, if performed on an amount of nucleic acid at or over a threshold amount and/or on nucleic acids at or below a threshold concentration. The threshold amount and concentration can vary depending on the nature and source of the nucleic acids to be amplified and the type of amplification reaction employed. Disclosed is a method of determining the threshold amount and/or threshold concentration of nucleic acids that can be used with nucleic acid samples of interest in amplification reactions of interest. Because amplification reactions can produce high quality amplification products, such as low bias amplification products, below the threshold amount and/or concentration of nucleic acid, such below-threshold amounts and/or concentrations can be used in amplification reactions.Type: ApplicationFiled: October 12, 2007Publication date: June 5, 2008Inventors: Carole Bornarth, Michele Wisniewski, Seiyu Hosono, Arumugham Raghunathan, Roger S. Lasken
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Patent number: 7297485Abstract: Disclosed are compositions and methods for amplification of nucleic acid sequences of interest. It has been discovered that amplification reactions can produce amplification products of high quality, such as low amplification bias, if performed on an amount of nucleic acid at or over a threshold amount and/or on nucleic acids at or below a threshold concentration. The threshold amount and concentration can vary depending on the nature and source of the nucleic acids to be amplified and the type of amplification reaction employed. Disclosed is a method of determining the threshold amount and/or threshold concentration of nucleic acids that can be used with nucleic acid samples of interest in amplification reactions of interest. Because amplification reactions can produce high quality amplification products, such as low bias amplification products, below the threshold amount and/or concentration of nucleic acid, such below-threshold amounts and/or concentrations can be used in amplification reactions.Type: GrantFiled: May 2, 2003Date of Patent: November 20, 2007Assignee: QIAGEN GmbHInventors: Carole Bornarth, Michele Wisniewski, Seiyu Hosono, Arumugham Raghunathan, Roger S. Lasken
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Publication number: 20030228613Abstract: Disclosed are compositions and methods for amplification of nucleic acid sequences of interest. It has been discovered that amplification reactions can produce amplification products of high quality, such as low amplification bias, if performed on an amount of nucleic acid at or over a threshold amount and/or on nucleic acids at or below a threshold concentration. The threshold amount and concentration can vary depending on the nature and source of the nucleic acids to be amplified and the type of amplification reaction employed. Disclosed is a method of determining the threshold amount and/or threshold concentration of nucleic acids that can be used with nucleic acid samples of interest in amplification reactions of interest. Because amplification reactions can produce high quality amplification products, such as low bias amplification products, below the threshold amount and/or concentration of nucleic acid, such below-threshold amounts and/or concentrations can be used in amplification reactions.Type: ApplicationFiled: May 2, 2003Publication date: December 11, 2003Inventors: Carole Bornarth, Michele Wisniewski, Seiyu Hosono, Arumugham Raghunathan, Roger S. Lasken