Abstract: A method for culturing 3-dimensional sections of CNS tissue, such as brain tissue, under microgravity conditions is disclosed. In one example, the microgravity conditions are simulated microgravity conditions generated by a culture vessel containing said tissue. Brain tissue cultured under simulated microgravity conditions as described herein remain viable, retain natural tissue architecture and organization and respond to well characterized electrophysiological stimuli in the same manner as brain tissue studied under acute conditions. The culture method described may be used for any brain tissue desired. The brain tissues and cells generated by the method of the instant disclosure may be used for a variety of purposes, including, but not limited to, development of disease models, physiological models, drug screening and drug evaluation.