Patents by Inventor Caroline DENESVRE

Caroline DENESVRE has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for selecting a permissive cell line for replicating avian viruses
    Patent number: 10053672
    Abstract: The present invention relates to a method for obtaining an untransformed avian cell line enabling in vitro avian virus replication. Said method includes the following steps: a) culturing avian embryonic stem cells in the presence of a stroma for at least 3 days; b) culturing for at least 2 days in a medium having a low serum concentration; c) culturing for at least 2 days in a medium having a low serum concentration containing 1 to 10 mM of hexamethyleme bisacetamide (HMBA); d) culturing for at least 10 days in a medium having a low serum concentration; and e) culturing or freezing an avian cell line enabling avian virus replication. The invention also relates to the resulting cell line and to the use thereof in vaccine preparations.
    Type: Grant
    Filed: July 24, 2014
    Date of Patent: August 21, 2018
    Assignee: INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE
    Inventors: Jean-François Vautherot, Bertrand Pain, Caroline Denesvre, Laetitia Fragnet-Trapp
  • METHOD FOR SELECTING A PERMISSIVE CELL LINE FOR REPLICATING AVIAN VIRUSES
    Publication number: 20160152956
    Abstract: The present invention relates to a method for obtaining an untransformed avian cell line enabling in vitro avian virus replication. Said method includes the following steps: a) culturing avian embryonic stem cells in the presence of a stroma for at least 3 days; b) culturing for at least 2 days in a medium having a low serum concentration; c) culturing for at least 2 days in a medium having a low serum concentration containing 1 to 10 mM of hexamethyleme bisacetamide (HMBA); d) culturing for at least 10 days in a medium having a low serum concentration; and e) culturing or freezing an avian cell line enabling avian virus replication. The invention also relates to the resulting cell line and to the use thereof in vaccine preparations.
    Type: Application
    Filed: July 24, 2014
    Publication date: June 2, 2016
    Inventors: Jean-François VAUTHEROT, Bertrand PAIN, Caroline DENESVRE, Laetitia FRAGNET-TRAPP