Patents by Inventor Carolyn R. Bertozzi
Carolyn R. Bertozzi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7923582Abstract: The present invention features a chemoselective ligation reaction that can be carried out under physiological conditions. In general, the invention involves condensation of a specifically engineered phosphine, which can provide for formation of an amide bond between the two reactive partners resulting in a final product comprising a phosphine moiety, or which can be engineered to comprise a cleavable linker so that a substituent of the phosphine is transferred to the azide, releasing an oxidized phosphine byproduct and producing a native amide bond in the final product. The selectivity of the reaction and its compatibility with aqueous environments provides for its application in vivo (e.g., on the cell surface or intracellularly) and in vitro (e.g., synthesis of peptides and other polymers, production of modified (e.g., labeled) amino acids).Type: GrantFiled: January 19, 2005Date of Patent: April 12, 2011Assignee: The Regents of the University of CaliforniaInventors: Eliana Saxon, Carolyn R. Bertozzi
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Patent number: 7868152Abstract: The invention provides anti-sulfotyrosine specific antibodies capable of detecting and isolating polypeptides that are tyrosine-sulfated. The sulfotyrosine antibodies and antibody fragments of the invention may be used to discriminate between the non-sulfated and sulfated forms of such proteins, using any number of immunological assays, such ELISAs, immunoblots, Western Blots, immunoprecipitations, and the like. Using a phage-display system, single chain antibodies (scFvs) were generated and screened against tyrosine-sulfated synthetic peptide antigens, resulting in the isolation of scFvs that specifically recognize sulfotyrosine-containing peptides and/or demonstrate sulfotyrosine-specific binding in tyrosine sulfated proteins. The VH and VL genes from one such sulfotyrosine-specific scFv were employed to generate a full length, sulfotyrosine-specific immunoglobulin.Type: GrantFiled: September 15, 2009Date of Patent: January 11, 2011Assignee: Los Alamos National Security, LLCInventors: Carolyn R. Bertozzi, John Kehoe, Andrew M. Bradbury
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Patent number: 7807619Abstract: The present invention provides modified cycloalkyne compounds; and method of use of such compounds in modifying biomolecules. The present invention features a cycloaddition reaction that can be carried out under physiological conditions. In general, the invention involves reacting a modified cycloalkyne with an azide moiety on a target biomolecule, generating a covalently modified biomolecule. The selectivity of the reaction and its compatibility with aqueous environments provide for its application in vivo (e.g., on the cell surface or intracellularly) and in vitro (e.g., synthesis of peptides and other polymers, production of modified (e.g., labeled) amino acids).Type: GrantFiled: October 31, 2005Date of Patent: October 5, 2010Assignee: The Regents of the University of CaliforniaInventors: Carolyn R. Bertozzi, Nicholas J. Agard, Jennifer A. Prescher, Jeremy Michael Baskin
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Patent number: 7667012Abstract: The present invention features a chemoselective ligation reaction that can be carried out under physiological conditions. In general, the invention involves condensation of a specifically engineered phosphine, which can provide for formation of an amide bond between the two reactive partners resulting in a final product comprising a phosphine moiety, or which can be engineered to comprise a cleavable linker so that a substituent of the phosphine is transferred to the azide, releasing an oxidized phosphine byproduct and producing a native amide bond in the final product. The selectivity of the reaction and its compatibility with aqueous environments provides for its application in vivo (e.g. on the cell surface or intracellularly) and in vitro (e.g., synthesis of peptides and other polymers, production of modified (e.g., labeled) amino acids).Type: GrantFiled: April 20, 2006Date of Patent: February 23, 2010Assignee: The Regents of the University of CaliforniaInventors: Eliana Saxon, Carolyn R. Bertozzi
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Publication number: 20100009441Abstract: The invention provides anti-sulfotyrosine specific antibodies capable of detecting and isolating polypeptides that are tyrosine-sulfated. The sulfotyrosine antibodies and antibody fragments of the invention may be used to discriminate between the non-sulfated and sulfated forms of such proteins, using any number of immunological assays, such ELISAs, immunoblots, Western Blots, immunoprecipitations, and the like. Using a phage-display system, single chain antibodies (scFvs) were generated and screened against tyrosine-sulfated synthetic peptide antigens, resulting in the isolation of scFvs that specifically recognize sulfotyrosine-containing peptides and/or demonstrate sulfotyrosine-specific binding in tyrosine sulfated proteins. The VH and VL genes from one such sulfotyrosine-specific scFv were employed to generate a full length, sulfotyrosine-specific immunoglobulin.Type: ApplicationFiled: September 15, 2009Publication date: January 14, 2010Applicant: LOS ALAMOS NATIONAL LABORATORYInventors: Carolyn R. Bertozzi, John Kehoe, Andrew M. Bradbury
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Patent number: 7589182Abstract: The invention provides anti-sulfotyrosine specific antibodies capable of detecting and isolating polypeptides that are tyrosine-sulfated. The sulfotyrosine antibodies and antibody fragments of the invention may be used to discriminate between the non-sulfated and sulfated forms of such proteins, using any number of immunological assays, such ELISAs, immunoblots, Western Blots, immunoprecipitations, and the like. Using a phage-display system, single chain antibodies (scFvs) were generated and screened against tyrosine-sulfated synthetic peptide antigens, resulting in the isolation of scFvs that specifically recognize sulfotyrosine-containing peptides and/or demonstrate sulfotyrosine-specific binding in tyrosine sulfated proteins. The VH and VL genes from one such sulfotyrosine-specific scFv were employed to generate a full length, sulfotyrosine-specific immunoglobulin.Type: GrantFiled: September 12, 2007Date of Patent: September 15, 2009Assignee: Los Alamos National Security, LLCInventors: Carolyn R. Bertozzi, John Kehoe, Andrew M. Bradbury
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Publication number: 20080279908Abstract: Hydroxyapatite (HA)-binding peptides are selected using combinatorial phage library display. Pseudo-repetitive consensus amino acid sequences possessing periodic hydroxyl side chains in every two or three amino acid sequences are obtained. These sequences resemble the (Gly-Pro-Hyp)x repeat of human type I collagen, a major component of extracellular matrices of natural bone. A consistent presence of basic amino acid residues is also observed. The peptides are synthesized by the solid-phase synthetic method and then used for template-driven HA-mineralization. Microscopy reveal that the peptides template the growth of polycrystalline HA crystals ˜40 nm in size.Type: ApplicationFiled: November 29, 2005Publication date: November 13, 2008Inventors: Carolyn R. Bertozzi, Jie Song, Seung-Wuk Lee
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Publication number: 20080275171Abstract: A composite having a flexible hydrogel polymer formed by mixing an organic phase with an inorganic composition, the organic phase selected from the group consisting of a hydrogel monomer, a crosslinker, a radical initiator, and/or a solvent. A polymerization mixture is formed and polymerized into a desired shape and size.Type: ApplicationFiled: February 22, 2006Publication date: November 6, 2008Applicant: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Jie Song, Eduardo Saiz, Carolyn R. Bertozzi, Antoni P. Tomsia
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Publication number: 20080199399Abstract: Disclosed herein are methods and materials by which nanostructures such as carbon nanotubes, nanorods, etc. are bound to lectins and/or polysaccharides and prepared for administration to cells. Also disclosed are complexes comprising glycosylated nanostructures, which bind selectively to cells expressing glycosylated surface molecules recognized by the lectin. Exemplified is a complex comprising a carbon nanotube functionalized with a lipid-like alkane, linked to a polymer bearing repeated ?-N-acetylgalactosamine sugar groups. This complex is shown to selectively adhere to the surface of living cells, without toxicity. In the exemplified embodiment, adherence is mediated by a multivalent lectin, which binds both to the cells and the ?-N-acetylgalactosamine groups on the nanostructure.Type: ApplicationFiled: February 20, 2008Publication date: August 21, 2008Inventors: Xing Chen, Carolyn R. Bertozzi, Alexander K. Zettl
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Publication number: 20080175869Abstract: Novel mycobacterial sulfation pathway proteins and polypeptides related thereto, as well as nucleic acid compositions encoding the same, are provided. The subject polypeptide and nucleic acid compositions find use in a variety of applications, including research, diagnostic, and therapeutic agent screening applications. Also provided are methods of inhibiting growth and/or virulence of a pathogenic mycobacterium, and methods of treating disease conditions associated with a pathogenic mycobacterium, particularly by administering an inhibitor of a mycobacterial sulfation pathway protein. The present invention further provides genetically modified mycobacteria having a defect in a sulfation pathway enzyme gene; and immunogenic compositions that include such genetically modified mycobacteria.Type: ApplicationFiled: October 18, 2007Publication date: July 24, 2008Inventors: Carolyn R. BERTOZZI, Spencer J. WILLIAMS, Joseph D. MOUGOUS
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Patent number: 7344703Abstract: Novel mycobacterial sulfation pathway proteins and polypeptides related thereto, as well as nucleic acid compositions encoding the same, are provided. The subject polypeptide and nucleic acid compositions find use in a variety of applications, including research, diagnostic, and therapeutic agent screening applications. Also provided are methods of inhibiting growth and/or virulence of a pathogenic mycobacterium, and methods of treating disease conditions associated with a pathogenic mycobacterium, particularly by administering an inhibitor of a mycobacterial sulfation pathway protein. The present invention further provides genetically modified mycobacteria having a defect in a sulfation pathway enzyme gene; and immunogenic compositions that include such genetically modified mycobacteria.Type: GrantFiled: September 1, 2005Date of Patent: March 18, 2008Assignee: The Regents of the University of CaliforniaInventors: Carolyn R. Bertozzi, Spencer J. Williams, Joseph D. Mougous
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Patent number: 7193034Abstract: The invention provides methods and compositions useful for making synthetic peptide conjugates. In one embodiment, the invention provides compositions comprising the structure: wherein R is selected from lower substituted or unsubstituted alkyl, O, NH and S and P is an amine protection group. In more particular embodiments, the compositions comprise ?-amine protected 4,5-dehydroleucine or ?-amine protected (2S)-aminolevulinic acid and/or P is F-moc. These compounds may be incorporated into peptides, for example, peptides comprising a substituted or unsubstituted (2S)-aminolevulinic acid residue, such as (2S)-aminolevulinic acid residue is substituted with an O- or N-linked glycoconjugate, or a detectable label.Type: GrantFiled: September 6, 2005Date of Patent: March 20, 2007Assignee: Regents of the University of CaliforniaInventor: Carolyn R. Bertozzi
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Patent number: 6974580Abstract: Novel mycobacterial sulfation pathway proteins and polypeptides related thereto, as well as nucleic acid compositions encoding the same, are provided. The subject polypeptide and nucleic acid compositions find use in a variety of applications, including research, diagnostic, and therapeutic agent screening applications. Also provided are methods of inhibiting growth and/or virulence of a pathogenic mycobacterium, and methods of treating disease conditions associated with a pathogenic mycobacterium, particularly by administering an inhibitor of a mycobacterial sulfation pathway protein. The present invention further provides genetically modified mycobacteria having a defect in a sulfation pathway enzyme gene; and immunogenic compositions that include such genetically modified mycobacteria.Type: GrantFiled: July 13, 2004Date of Patent: December 13, 2005Assignee: The Regents of the University of CaliforniaInventors: Carolyn R. Bertozzi, Spencer J. Williams, Joseph D. Mougous
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Patent number: 6939945Abstract: The invention provides methods and compositions useful for making synthetic peptide conjugates. In one embodiment, the invention provides compositions comprising the structure: wherein R is selected from lower substituted or unsubstituted alkyl, O, NH and S and P is an amine protection group. In more particular embodiments, the compositions comprise ?-amine protected 4,5-dehydroleucine or ?-amine protected (2S)-aminolevulinic acid and/or P is F-moc. These compounds may be incorporated into peptides, for example, peptides comprising a substituted or unsubstituted (2S)-aminolevulinic acid residue, such as (2S)-aminolevulinic acid residue is substituted with an O- or N-linked glycoconjugate, or a detectable label.Type: GrantFiled: October 10, 2002Date of Patent: September 6, 2005Assignee: The Regents of the University of CaliforniaInventors: Carolyn R. Bertozzi, Lisa A. Marcaurelle, Elena C. Rodriguez
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Patent number: 6863895Abstract: Novel mycobacterial sulfation pathway proteins and polypeptides related thereto, as well as nucleic acid compositions encoding the same, are provided. The subject polypeptide and nucleic acid compositions find use in a variety of applications, including research, diagnostic, and therapeutic agent screening applications. Also provided are methods of inhibiting growth and/or virulence of a pathogenic mycobacterium, and methods of treating disease conditions associated with a pathogenic mycobacterium, particularly by administering an inhibitor of a mycobacterial sulfation pathway protein. The present invention further provides genetically modified mycobacteria having a defect in a sulfation pathway enzyme gene; and immunogenic compositions that include such genetically modified mycobacteria.Type: GrantFiled: October 31, 2002Date of Patent: March 8, 2005Assignee: The Regents of the University of CaliforniaInventors: Carolyn R. Bertozzi, Spencer J. Williams, Joseph D. Mougous
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Patent number: 6858213Abstract: Novel mycobacterial sulfation pathway enzymes and polypeptides related thereto, as well as nucleic acid compositions encoding the same, are provided. The subject polypeptide and nucleic acid compositions find use in a variety of applications, including research, diagnostic, and therapeutic agent screening applications. Also provided are methods of inhibiting growth and/or virulence of a pathogenic mycobacterium, and methods of treating disease conditions associated with a pathogenic mycobacterium, particularly by administering an inhibitor of a mycobacterial sulfation pathway enzyme.Type: GrantFiled: April 19, 2002Date of Patent: February 22, 2005Assignee: The Regents of the University of CaliforniaInventors: Carolyn R. Bertozzi, Spencer J. Williams, Joseph D. Mougous
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Publication number: 20040249131Abstract: Novel mycobacterial sulfation pathway proteins and polypeptides related thereto, as well as nucleic acid compositions encoding the same, are provided. The subject polypeptide and nucleic acid compositions find use in a variety of applications, including research, diagnostic, and therapeutic agent screening applications. Also provided are methods of inhibiting growth and/or virulence of a pathogenic mycobacterium, and methods of treating disease conditions associated with a pathogenic mycobacterium, particularly by administering an inhibitor of a mycobacterial sulfation pathway protein. The present invention further provides genetically modified mycobacteria having a defect in a sulfation pathway enzyme gene; and immunogenic compositions that include such genetically modified mycobacteria.Type: ApplicationFiled: July 13, 2004Publication date: December 9, 2004Applicant: The Regents of the University of CaliforniaInventors: Carolyn R. Bertozzi, Spencer J. Williams, Joseph D. Mougous
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Publication number: 20040161444Abstract: A template-driven biomineralization process for making three-dimensional bonelike composites having direct and extensive mineral-substrate contact which provides high adhesion strength. The in situ generation of sufficient amounts of surface and interior carboxylates, through an increase in pH, serves as nuclear binding sites for mineral ions to promote high affinity 2-dimensional mineral growth at the substrate-mineral interface. The substrate for the bonelike composites is a hydrogel scaffold comprised of a polymerized base monomer having hydrolyzable ester side chains, crosslinked with a co-monomer and crosslinker. Hydrolysis of the ester containing side chains is preferably mediated by thermo-decomposition of urea.Type: ApplicationFiled: December 18, 2003Publication date: August 19, 2004Applicant: The Regents of the University of CaliforniaInventors: Jie Song, Carolyn R. Bertozzi, Eduardo Saiz
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Publication number: 20030180321Abstract: Novel mycobacterial sulfation pathway proteins and polypeptides related thereto, as well as nucleic acid compositions encoding the same, are provided. The subject polypeptide and nucleic acid compositions find use in a variety of applications, including research, diagnostic, and therapeutic agent screening applications. Also provided are methods of inhibiting growth and/or virulence of a pathogenic mycobacterium, and methods of treating disease conditions associated with a pathogenic mycobacterium, particularly by administering an inhibitor of a mycobacterial sulfation pathway protein. The present invention further provides genetically modified mycobacteria having a defect in a sulfation pathway enzyme gene; and immunogenic compositions that include such genetically modified mycobacteria.Type: ApplicationFiled: October 31, 2002Publication date: September 25, 2003Inventors: Carolyn R. Bertozzi, Spencer J. Williams, Joseph D. Mougous
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Publication number: 20030104001Abstract: Novel mycobacterial sulfation pathway enzymes and polypeptides related thereto, as well as nucleic acid compositions encoding the same, are provided. The subject polypeptide and nucleic acid compositions find use in a variety of applications, including research, diagnostic, and therapeutic agent screening applications. Also provided are methods of inhibiting growth and/or virulence of a pathogenic mycobacterium, and methods of treating disease conditions associated with a pathogenic mycobacterium, particularly by administering an inhibitor of a mycobacterial sulfation pathway enzyme.Type: ApplicationFiled: April 19, 2002Publication date: June 5, 2003Inventors: Carolyn R. Bertozzi, Spencer J. Williams, Joseph D. Mougous