Patents by Inventor Carolyn Woodroofe Hitko
Carolyn Woodroofe Hitko has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230183603Abstract: The invention provides surfactant compounds of formulas I-IX, which can be used in methods for aiding the solubilization, digestion, preparation, analysis, and/or characterization of biological material, for example, proteins or cell membranes. The compounds can also aid in the recovery of peptides generated during protein digestion, particularly for in-gel digestion protocol. Additionally, the compounds can improve enzymatic protein deglycosylation without interfering with downstream sample preparation steps and mass spectrometric analysis. The compounds can be specifically useful as digestion aids that can be decomposed by an acid, by heat, or a combination thereof. Decomposition of the surfactants allows for facile separation from isolated samples, and/or allows for analysis of the sample without interfering with the sensitivity of various analytical techniques.Type: ApplicationFiled: October 10, 2022Publication date: June 15, 2023Inventors: Sergei Saveliev, Daniel Simpson, Keith V. Wood, Harry Tetsuo Uyeda, Carolyn Woodroofe Hitko, Dieter Klaubert
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Patent number: 11624041Abstract: The invention provides surfactant compounds of formulas I-IX, which can be used in methods for aiding the solubilization, digestion, preparation, analysis, and/or characterization of biological material, for example, proteins or cell membranes. The compounds can also aid in the recovery of peptides generated during protein digestion, particularly for in-gel digestion protocol. Additionally, the compounds can improve enzymatic protein deglycosylation without interfering with downstream sample preparation steps and mass spectrometric analysis. The compounds can be specifically useful as digestion aids that can be decomposed by an acid, by heat, or a combination thereof. Decomposition of the surfactants allows for facile separation from isolated samples, and/or allows for analysis of the sample without interfering with the sensitivity of various analytical techniques.Type: GrantFiled: February 28, 2020Date of Patent: April 11, 2023Assignee: Promega CorporationInventors: Sergei Saveliev, Daniel Simpson, Keith V. Wood, Harry Tetsuo Uyeda, Carolyn Woodroofe Hitko, Dieter Klaubert
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Publication number: 20200263110Abstract: The invention provides surfactant compounds of formulas I-IX, which can be used in methods for aiding the solubilization, digestion, preparation, analysis, and/or characterization of biological material, for example, proteins or cell membranes. The compounds can also aid in the recovery of peptides generated during protein digestion, particularly for in-gel digestion protocol. Additionally, the compounds can improve enzymatic protein deglycosylation without interfering with downstream sample preparation steps and mass spectrometric analysis. The compounds can be specifically useful as digestion aids that can be decomposed by an acid, by heat, or a combination thereof. Decomposition of the surfactants allows for facile separation from isolated samples, and/or allows for analysis of the sample without interfering with the sensitivity of various analytical techniques.Type: ApplicationFiled: February 28, 2020Publication date: August 20, 2020Inventors: Sergei Saveliev, Daniel Simpson, Keith V. Wood, Harry Tetsuo Uyeda, Carolyn Woodroofe Hitko, Dieter Klaubert
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Patent number: 10626349Abstract: The invention provides surfactant compounds of formulas I-IX, which can be used in methods for aiding the solubilization, digestion, preparation, analysis, and/or characterization of biological material, for example, proteins or cell membranes. The compounds can also aid in the recovery of peptides generated during protein digestion, particularly for in-gel digestion protocol. Additionally, the compounds can improve enzymatic protein deglycosylation without interfering with downstream sample preparation steps and mass spectrometric analysis. The compounds can be specifically useful as digestion aids that can be decomposed by an acid, by heat, or a combination thereof. Decomposition of the surfactants allows for facile separation from isolated samples, and/or allows for analysis of the sample without interfering with the sensitivity of various analytical techniques.Type: GrantFiled: October 10, 2017Date of Patent: April 21, 2020Assignee: Promega CorporationInventors: Sergei Saveliev, Daniel Simpson, Keith V. Wood, Harry Tetsuo Uyeda, Carolyn Woodroofe Hitko, Dieter Klaubert
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Publication number: 20180187127Abstract: The invention provides surfactant compounds of formulas I-IX, which can be used in methods for aiding the solubilization, digestion, preparation, analysis, and/or characterization of biological material, for example, proteins or cell membranes. The compounds can also aid in the recovery of peptides generated during protein digestion, particularly for in-gel digestion protocol. Additionally, the compounds can improve enzymatic protein deglycosylation without interfering with downstream sample preparation steps and mass spectrometric analysis. The compounds can be specifically useful as digestion aids that can be decomposed by an acid, by heat, or a combination thereof. Decomposition of the surfactants allows for facile separation from isolated samples, and/or allows for analysis of the sample without interfering with the sensitivity of various analytical techniques.Type: ApplicationFiled: October 10, 2017Publication date: July 5, 2018Inventors: Sergei Saveliev, Daniel Simpson, Keith V. Wood, Harry Tetsuo Uyeda, Carolyn Woodroofe Hitko, Dieter Klaubert
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Patent number: 9868977Abstract: Novel red-shifted luciferin derivatives and uses of those compounds are provided.Type: GrantFiled: September 19, 2016Date of Patent: January 16, 2018Assignee: PROMEGA CORPORATIONInventor: Carolyn Woodroofe Hitko
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Patent number: 9816054Abstract: The invention provides surfactant compounds of formulas I-IX, which can be used in methods for aiding the solubilization, digestion, preparation, analysis, and/or characterization of biological material, for example, proteins or cell membranes. The compounds can also aid in the recovery of peptides generated during protein digestion, particularly for in-gel digestion protocol. Additionally, the compounds can improve enzymatic protein deglycosylation without interfering with downstream sample preparation steps and mass spectrometric analysis. The compounds can be specifically useful as digestion aids that can be decomposed by an acid, by heat, or a combination thereof. Decomposition of the surfactants allows for facile separation from isolated samples, and/or allows for analysis of the sample without interfering with the sensitivity of various analytical techniques.Type: GrantFiled: October 10, 2008Date of Patent: November 14, 2017Assignee: PROMEGA CORPORATIONInventors: Sergei Saveliev, Daniel Simpson, Keith V. Wood, Harry Tetsuo Uyeda, Carolyn Woodroofe Hitko, Dieter Klaubert
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Publication number: 20170002396Abstract: Novel red-shifted luciferin derivatives and uses of those compounds are provided.Type: ApplicationFiled: September 19, 2016Publication date: January 5, 2017Inventor: Carolyn Woodroofe Hitko
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Patent number: 9447450Abstract: Novel red-shifted luciferin derivatives and uses of those compounds are provided.Type: GrantFiled: March 7, 2014Date of Patent: September 20, 2016Assignee: PROMEGA CORPORATIONInventor: Carolyn Woodroofe Hitko
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Patent number: 8962266Abstract: The present invention provides compounds and methods for assaying activities of enzymes such as histone deacetylases and histone acetyltransferases. In some embodiments, the methods may be performed in one step. The compounds described herein features peptide-based compounds having at least one blocked lysine or arginine residue which are coupled to reporter moieties. The methods described herein involve reacting a compound described herein with an enzyme, such as a histone deacetylase enzyme or a histone acetyltransferase enzyme, and an endopeptidase that recognizes basic amino acids to release the reporter moiety which may be subsequently detected.Type: GrantFiled: November 26, 2013Date of Patent: February 24, 2015Assignee: Promega CorporationInventors: Thomas Kirkland, Andrew L. Niles, Martha O'Brien, Carolyn Woodroofe Hitko
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Publication number: 20140304842Abstract: Novel red-shifted luciferin derivatives and uses of those compounds are provided.Type: ApplicationFiled: March 7, 2014Publication date: October 9, 2014Applicant: PROMEGA CORPORATIONInventor: Carolyn Woodroofe Hitko
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Publication number: 20140099655Abstract: The present invention provides compounds and methods for assaying activities of enzymes such as histone deacetylases and histone acetyltransferases. In some embodiments, the methods may be performed in one step. The compounds described herein features peptide-based compounds having at least one blocked lysine or arginine residue which are coupled to reporter moieties. The methods described herein involve reacting a compound described herein with an enzyme, such as a histone deacetylase enzyme or a histone acetyltransferase enzyme, and an endopeptidase that recognizes basic amino acids to release the reporter moiety which may be subsequently detected.Type: ApplicationFiled: November 26, 2013Publication date: April 10, 2014Applicant: Promega CorporationInventors: Thomas Kirkland, Andrew L. Niles, Martha O'Brien, Carolyn Woodroofe Hitko
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Patent number: 8632992Abstract: The present invention provides compounds and methods for assaying activities of enzymes such as histone deacetylases and histone acetyltransferases. In some embodiments, the methods may be performed in one step. The compounds described herein features peptide-based compounds having at least one blocked lysine or arginine residue which are coupled to reporter moieties. The methods described herein involve reacting a compound described herein with an enzyme, such as a histone deacetylase enzyme or a histone acetyltransferase enzyme, and an endopeptidase that recognizes basic amino acids to release the reporter moiety which may be subsequently detected.Type: GrantFiled: September 9, 2011Date of Patent: January 21, 2014Assignee: Promega CorporationInventors: Thomas Kirkland, Andrew L. Niles, Carolyn Woodroofe Hitko
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Publication number: 20120064554Abstract: The present invention provides compounds and methods for assaying activities of enzymes such as histone deacetylases and histone acetyltransferases. In some embodiments, the methods may be performed in one step. The compounds described herein features peptide-based compounds having at least one blocked lysine or arginine residue which are coupled to reporter moieties. The methods described herein involve reacting a compound described herein with an enzyme, such as a histone deacetylase enzyme or a histone acetyltransferase enzyme, and an endopeptidase that recognizes basic amino acids to release the reporter moiety which may be subsequently detected.Type: ApplicationFiled: September 9, 2011Publication date: March 15, 2012Applicant: PROMEGA CORPORATIONInventors: Thomas Kirkland, Andrew L. Niles, Martha O'Brien, Carolyn Woodroofe Hitko