Patents by Inventor Carsten Lillelund Olsen
Carsten Lillelund Olsen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220073898Abstract: The present invention provides means and methods for improving polypeptide expression yield by increasing the number of polypeptides that can be transported into the endoplasmic reticulum (ER) per translocation event.Type: ApplicationFiled: December 17, 2019Publication date: March 10, 2022Applicant: Novozymes A/SInventors: Richard Jan Steven Baerends, Carsten Lillelund Olsen, Josè Arnau
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Patent number: 10351863Abstract: The present invention relates to methods for constructing a recombinant fungal host cell comprising one or more copies of a polynucleotide construct integrated in its genome, said method comprising transforming a fungal host cell with an integrative polynucleotide construct comprising a first polynucleotide encoding a selectable marker, wherein the first polynucleotide, a 5? untranslated region thereof and/or a riboswitch operably linked therewith comprises a spliceosomal intron which has 5 nucleotides or less between its branch site and its acceptor site; and a second polynucleotide encoding a polypeptide of interest; as well as suitable polynucleotide constructs, resulting fungal host cells and methods of manufacture.Type: GrantFiled: June 26, 2018Date of Patent: July 16, 2019Assignee: Novozymes A/SInventor: Carsten Lillelund Olsen
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Patent number: 10351861Abstract: The present invention relates to methods for constructing a recombinant fungal host cell comprising one or more copies of a polynucleotide construct integrated in its genome, said method comprising transforming a fungal host cell with an integrative polynucleotide construct comprising a first polynucleotide encoding a selectable marker, wherein the first polynucleotide, a 5? untranslated region thereof and/or a riboswitch operably linked therewith comprises a spliceosomal intron which has 5 nucleotides or less between its branch site and its acceptor site; and a second polynucleotide encoding a polypeptide of interest; as well as suitable polynucleotide constructs, resulting fungal host cells and methods of manufacture.Type: GrantFiled: October 18, 2016Date of Patent: July 16, 2019Assignee: Novozymes A/SInventor: Carsten Lillelund Olsen
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Publication number: 20180298394Abstract: The present invention relates to methods for constructing a recombinant fungal host cell comprising one or more copies of a polynucleotide construct integrated in its genome, said method comprising transforming a fungal host cell with an integrative polynucleotide construct comprising a first polynucleotide encoding a selectable marker, wherein the first polynucleotide, a 5? untranslated region thereof and/or a riboswitch operably linked therewith comprises a spliceosomal intron which has 5 nucleotides or less between its branch site and its acceptor site; and a second polynucleotide encoding a polypeptide of interest; as well as suitable polynucleotide constructs, resulting fungal host cells and methods of manufacture.Type: ApplicationFiled: June 26, 2018Publication date: October 18, 2018Applicant: Novozymes A/SInventor: Carsten Lillelund Olsen
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Publication number: 20170029830Abstract: The present invention relates to methods for constructing a recombinant fungal host cell comprising one or more copies of a polynucleotide construct integrated in its genome, said method comprising transforming a fungal host cell with an integrative polynucleotide construct comprising a first polynucleotide encoding a selectable marker, wherein the first polynucleotide, a 5? untranslated region thereof and/or a riboswitch operably linked therewith comprises a spliceosomal intron which has 5 nucleotides or less between its branch site and its acceptor site; and a second polynucleotide encoding a polypeptide of interest; as well as suitable polynucleotide constructs, resulting fungal host cells and methods of manufacture.Type: ApplicationFiled: October 18, 2016Publication date: February 2, 2017Inventor: Carsten Lillelund Olsen
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Patent number: 9487767Abstract: The present invention relates to methods for constructing a recombinant fungal host cell comprising one or more copies of a polynucleotide construct integrated in its genome, said method comprising transforming a fungal host cell with an integrative polynucleotide construct comprising a first polynucleotide encoding a selectable marker, wherein the first polynucleotide, a 5? untranslated region thereof and/or a riboswitch operably linked therewith comprises a spliceosomal intron which has 5 nucleotides or less between its branch site and its acceptor site; and a second polynucleotide encoding a polypeptide of interest; as well as suitable polynucleotide constructs, resulting fungal host cells and methods of manufacture.Type: GrantFiled: May 29, 2013Date of Patent: November 8, 2016Assignee: Novozymes A/S of Krogshoejvej 36Inventor: Carsten Lillelund Olsen
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Publication number: 20160304905Abstract: The present invention relates to a method for site-specific chromosomal integration of a gene library in a filamentous fungal host cell and a polynucleotide construct suitable for this purpose.Type: ApplicationFiled: December 3, 2014Publication date: October 20, 2016Inventors: Bjarne G. Hansen, Carsten Lillelund Olsen, Jesper Vind
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Patent number: 9273320Abstract: The present invention relates to a method of producing a recombinant sweet protein in a filamentous fungus. The invention also relates to isolated polynucleotides encoding the sweet protein and to nucleic acid constructs, vectors, and host cells comprising the polynucleotides, as well as methods of using the sweet protein produced by the method of the invention.Type: GrantFiled: August 5, 2010Date of Patent: March 1, 2016Assignee: NOVOZYMES A/SInventors: Jesper Vind, Jeppe Wegener Tams, Lars Beier, Carsten Lillelund Olsen
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Publication number: 20150175981Abstract: The present invention relates to methods for constructing a recombinant fungal host cell comprising one or more copies of a polynucleotide construct integrated in its genome, said method comprising transforming a fungal host cell with an integrative polynucleotide construct comprising a first polynucleotide encoding a selectable marker, wherein the first polynucleotide, a 5? untranslated region thereof and/or a riboswitch operably linked therewith comprises a spliceosomal intron which has 5 nucleotides or less between its branch site and its acceptor site; and a second polynucleotide encoding a polypeptide of interest; as well as suitable polynucleotide constructs, resulting fungal host cells and methods of manufacture.Type: ApplicationFiled: May 29, 2013Publication date: June 25, 2015Applicant: Novozymes A/S of Krogshoejvej 36Inventor: Carsten Lillelund Olsen
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Publication number: 20120107873Abstract: The present invention relates to a method of producing a recombinant sweet protein in a filamentous fungus. The invention also relates to isolated polynucleotides encoding the sweet protein and to nucleic acid constructs, vectors, and host cells comprising the polynucleotides, as well as methods of using the sweet protein produced by the method of the invention.Type: ApplicationFiled: August 5, 2010Publication date: May 3, 2012Applicant: NOVOZYMES A/SInventors: Jesper Vind, Jeppe Wegener Tams, Lars Beier, Carsten Lillelund Olsen