Abstract: This invention relates to compounds comprising a polypeptide and a fluorophore, wherein the polypeptide is capable of being phosphorylated by a Cyclin-Dependent Kinase (CDK) and/or a Cyclin-CDK complex on a specific site and wherein the fluorescence of said fluorophore changes upon phosphorylation of said specific site of said polypeptide. The invention also relates to the use of such compounds or of compositions comprising such compounds for medical imaging. The invention furthermore relates to methods for determining if at least one CDK and/or Cyclin-CDK complex is active, for determining changes in at least one CDK and/or Cyclin-CDK complex activity, for the in vitro diagnosis of Cyclin-dependent kinase hyperactivation in a subject.

Abstract: The présent invention relates to cell-penetrating peptides characterized in that it comprises an amino acid séquence selected in the group consisting of X1LX2RALWX9LX3X9X4LWX9LX5X6X7X8, X1LX2LARWX9LX3X9X4LWX9LX5X6X7X8 and X1LX2ARLWX9LX3X9X4LWX9LX5X6X7X8, wherein X1 is beta-A or S, X2 is F or W, X3 is L, W, C or I, X4 is S, A, N or T, Xs is L or W, X6 is W or R, X7 is K or R, X8 is A or none, and X9 is R or S. Thèse peptides exhibit high efficiency, low toxicity and a natural tropism for lung tissues, and can be used either in simple complex with the cargo to be vectorised, or in nanoparticles comprising two layers of cell-pentrating peptides around the cargo.

Abstract: Complex comprising a peptide carrier of SEQ ID NO:1 GALFLGFLGAAGSTMGAWSQPKR1KRKVR2 and an appropriate siRNA, wherein R1 represents any amino acid residue and more preferably K or S, R2 is null or represents one of the following groups: cysteamide, cysteine, thiol, amide, linear or ramified C1-C6 alkyl optionally substituted, primary or secondary amine, osidic derivative, lipid, phospholipid or cholesterol and said siRNA is selected to silence a target mRNA.

Abstract: The present invention concerns cell-penetrating peptides which comprise an amino acid sequence consisting of GLX9RALX9RX1LX2RSLX9X3X4X5X6X7X8 (SEQ ID No: 1), wherein X1 is A, L or G, X2 is W or none, X3 is R or K, X4 is K, L or S, X5 is L or K, X6 is R or W, X7 is K or S, and X8 is A, V or Q, and X9 is W, F or Y. These CPPs can be used as vectors for delivering nucleic acids and/or proteins and/or peptides to cells, in vitro or in vivo.

Abstract: The present invention concerns cell-penetrating peptides which comprise an amino acid sequence consisting of GLX9WRAX9WRX1LX2RSLX9WX3X4X5X6X7X8 (SEQ ID No: 1), wherein X1 is A, L or G, X2 is W or none, X3 is R or K, X4 is K, L or S, X5 is L or K, X6 is R or W, X7 is K or S, and X8 is A, V or Q, and X9 is W, F or Y. These CPPs can be used as vectors for delivering nucleic acids and/or proteins and/or peptides to cells, in vitro or in vivo.

Abstract: A mixing apparatus has a chamber, a shaft extending into the chamber, a motor drivingly interconnected to the shaft, a nozzle support affixed to the shaft and extending outwardly therefrom within the chamber, and a nozzle having an interior passageway affixed to the nozzle support such that the nozzle moves in the chamber as the motor drivingly rotates the shaft. The chamber has a multi-phase fluid therein. A flow restrictor is affixed to an inner wall of said chamber so as to extend inwardly therefor. The flow restrictor is a plurality of flat panels arranged in spaced relation around the interior of the chamber and within a liquid phase of the multi-phase fluid.

Abstract: A mixing apparatus has a chamber, a shaft extending into the chamber, a motor drivingly interconnected to the shaft, a nozzle support affixed to the shaft and extending outwardly therefrom within the chamber, and a nozzle having an interior passageway affixed to the nozzle support such that the nozzle moves in the chamber as the motor drivingly rotates the shaft. The chamber has a multi-phase fluid therein. The nozzle moves through the fluid such that fluid is channeled through the interior passageway at a same rate that the nozzle moves through the fluid. Another nozzle can be affixed to the nozzle support diametrically opposite to the nozzle.

Abstract: The present invention concerns cell-penetrating peptides which comprise an amino acid sequence consisting of GLX9RALX9RX1LX2RSLX9X3X4X5X6X7X8 (SEQ ID No: 1), wherein X1 is A, L or G, X2 is W or none, X3 is R or K, X4 is K, L or S, X5 is L or K, X6 is R or W, X7 is K or S, and X8 is A, V or Q, and X9 is W, F or Y. These CPPs can be used as vectors for delivering nucleic acids and/or proteins and/or peptides to cells, in vitro or in vivo.

Abstract: Complex comprising a peptide carrier of SEQ ID NO:1 GALFLGFLGAAGSTMGAWSQPKR1KRKVR2 and an appropriate siRNA, wherein R1 represents any amino acid residue and more preferably K or S, R2 is null or represents one of the following groups: cysteamide, cysteine, thiol, amide, linear or ramified C1-C6 alkyl optionally substituted, primary or secondary amine, osidic derivative, lipid, phospholipid or cholesterol and said siRNA is selected to silence a target mRNA.

Abstract: Complex comprising a peptide carrier of SEQ ID NO:1 GALFLGFLGAAGSTMGAWSQPKR1KRKVR2 and an appropriate siRNA, wherein R1 represents any amino acid residue and more preferably K or S, R2 is null or represents one of the following groups: cysteamide, cysteine, thiol, amide, linear or ramified C1-C6 alkyl optionally substituted, primary or secondary amine, osidic derivative, lipid, phospholipid or cholesterol and said siRNA is selected to silence a target mRNA.

Abstract: A mixing apparatus has a chamber, a shaft extending into the chamber, a motor drivingly interconnected to the shaft, a nozzle support affixed to the shaft and extending outwardly therefrom within the chamber, and a nozzle having an interior passageway affixed to the nozzle support such that the nozzle moves in the chamber as the motor drivingly rotates the shaft. The chamber has a multi-phase fluid therein. A flow restrictor is affixed to an inner wall of said chamber so as to extend inwardly therefor. The flow restrictor is a plurality of flat panels arranged in spaced relation around the interior of the chamber and within a liquid phase of the multi-phase fluid.

Abstract: A mixing apparatus has a chamber, a shaft extending into the chamber, a motor drivingly interconnected to the shaft, a nozzle support affixed to the shaft and extending outwardly therefrom within the chamber, and a nozzle having an interior passageway affixed to the nozzle support such that the nozzle moves in the chamber as the motor drivingly rotates the shaft. The chamber has a multi-phase fluid therein. The nozzle moves through the fluid such that fluid is channeled through the interior passageway at a same rate that the nozzle moves through the fluid. Another nozzle can be affixed to the nozzle support diametrically opposite to the nozzle.

Abstract: The present invention concerns cell-penetrating peptides which comprise an amino acid sequence consisting of GLX9WRAX9WRX1LX2RSLX9WX3X4X5X6X7X8 (SEQ ID No: 1), wherein X1 is A, L or G, X2 is W or none, X3 is R or K, X4 is K, L or S, X5 is L or K, X6 is R or W, X7 is K or S, and X8 is A, V or Q, and X9 is W, F or Y. These CPPs can be used as vectors for delivering nucleic acids and/or proteins and/or peptides to cells, in vitro or in vivo.

Abstract: A composite gel or aerogel is formed by commingling a particulate guest (such as a colloidal or dispersed (i.e., non-colloidal) solid or a powder) with a sol which is either about to gel or in which gelation has just started. After addition of the particulate, the mixture is then permitted to gel into a solid, gelled composite with open pores. This solid, gelled composite is then dried in a manner that prevents the collapse of open pores within the solid, gelled composite in which the gel acts as a “nanoglue” that holds the particles together.