Abstract: The disclosure relates to candles, including multi-wick candles, that contain one more non-ionic emulsifiers. The non-ionic emulsifier(s) can be contained in the candle base formulation and/or in the wick coating formulation. The candles exhibit one or more desirable properties, such as good flame height, good fragrance character, low mineral impurity levels and little or no discoloration.

Abstract: The disclosure relates to candles, including multi-wick candles, that contain one more non-ionic emulsifiers. The non-ionic emulsifier(s) can be contained in the candle base formulation and/or in the wick coating formulation. The candles exhibit one or more desirable properties, such as good flame height, good fragrance character, low mineral impurity levels and little or no discoloration.

Abstract: The disclosure relates to candles, including multi-wick candles, that contain one more non-ionic emulsifiers. The non-ionic emulsifier(s) can be contained in the candle base formulation and/or in the wick coating formulation. The candles exhibit one or more desirable properties, such as good flame height, good fragrance character, low mineral impurity levels and little or no discoloration.

Abstract: The invention provides a method for detecting a mycobacterium belonging to the Mycobacterium tuberculosis complex (MTBc) in a sample, the method comprising: (a) contacting the sample with a forward oligonucleotide primer and a reverse oligonucleotide primer; wherein the forward primer hybridizes to a target nucleic acid sequence located within a Mycobacterial Interspersed Repetitive Unit (MIRU) repeat element; and wherein the reverse primer hybridizes to a target nucleic acid sequence located within a MIRU repeat element; (b) extending the forward and reverse primers to generate an amplification product; and (c) detecting the amplification product.

Abstract: A method for detecting a mutation in a target nucleic acid sequence in a sample, the target nucleic acid sequence comprising a first DNA strand and optionally the complementary strand thereof, said method comprising: (a) adding a detection primer to the nucleic acid, wherein the detection primer binds to the first DNA strand at a DNA sequence that comprises the mutation site; (b) extending the detection primer to form second DNA strands that are complementary to the first DNA strand; (c) adding an amplification primer to the nucleic acid, wherein the amplification primer binds to the second DNA strand and/or to the complementary strand, at a position away from the mutation site; (d) extending the amplification primer to form third DNA strands that are complementary to the second DNA strands, and/or additional copies of the first DNA strand; (e) annealing the DNA strands by complementary base pairing, to form nucleic acid duplexes, wherein if the two strands of the duplex have a mismatched residue at the mutat

Abstract: The invention provides a method for detecting a mycobacterium belonging to the MTB complex in a sample, the method comprising: (a) contacting the sample with a pair of forward and reverse oligonucleotide primers; wherein said forward primer hybridises to a target nucleic acid sequence located within a Mycobacterial Interspersed Repetitive Unit (MIRU) repeat element; and wherein said reverse primer hybridises to a target nucleic acid sequence located within a Mycobacterial Interspersed Repetitive Unit (MIRU) repeat element; (b) extending said forward and reverse primers to generate an amplification product; and (c) detecting the amplification product.

Abstract: A method for detecting a mutation in a target nucleic acid sequence in a sample, the target nucleic acid sequence comprising a first DNA strand and optionally the complementary strand thereof, said method comprising: (a) adding a detection primer to the nucleic acid, wherein the detection primer binds to the first DNA strand at a DNA sequence that comprises the mutation site; (b) extending the detection primer to form second DNA strands that are complementary to the first DNA strand; (c) adding an amplification primer to the nucleic acid, wherein the amplification primer binds to the second DNA strand and/or to the complementary strand, at a position away from the mutation site; (d) extending the amplification primer to form third DNA strands that are complementary to the second DNA strands, and/or additional copies of the first DNA strand; (e) annealing the DNA strands by complementary base pairing, to form nucleic acid duplexes, wherein if the two strands of the duplex have a mismatched residue at the mutat