Abstract: A method of biomonitoring marine water is provided, as are probes and kits. The method comprises: extracting messenger Ribonucleic Acid (mRNA) from Mytilus species; preparing cDNA from the mRNA; hybridizing a plurality of probes with the cDNA to provide a set of hybridized cDNA, the probes consisting of at least 20 consecutive nucleotides of at least 50 sequences of SEQ ID NO: 1-122; and quantifying an abundance of the set of hybridized cDNA, thereby biomonitoring marine water.

Abstract: A method of biomonitoring marine water is provided, as are probes and kits. The method comprises: extracting messenger Ribonucleic Acid (mRNA) from Mytilus species; preparing cDNA from the mRNA; hybridizing a plurality of probes with the cDNA to provide a set of hybridized cDNA, the probes consisting of at least 20 consecutive nucleotides of at least 50 sequences of SEQ ID NO: 1-122; and quantifying an abundance of the set of hybridized cDNA, thereby biomonitoring marine water.

Abstract: Novel methods of detecting fluorescent proteins are described. The methods result in vastly improved signal-to-noise ratios in assays measuring fluorescence of a fluorescent protein specifically by employing a unique trapping step to microconcentrate the fluorescent protein and by using improved optical techniques. The trapping step may be a chemical or physical process or a combination thereof leading to substantial microconcentration of the fluorescent protein with concomitant removal of contaminants or interfering compounds. The methods are readily adaptable to high throughput screening and can be engineered for use with a wide variety of assays currently using microplate readers. Green fluorescent protein and fluorescent coral proteins are among preferred fluorescent proteins for the methods.

Abstract: Green fluorescent protein (GFP) polypeptides from Renilla reniformis and Renilla kollikeri are disclosed. The amino acid sequence of R. reniformis GFP and back-translated nucleotide sequences of nucleic acids encoding the R. reniformis GFP are also disclosed. These isolated polypeptides, along with the pertinent amino acid and nucleotide sequence information, are useful in a variety of applications for which GFPs from other sources (e.g., Aequoria) are currently employed. Techniques for using the Renilla GFPs are disclosed, along with advantages of Renilla GFP as compared with currently available GFPs.

Abstract: Green fluorescent protein (GFP) polypeptides from Renilla reniformis and Renilla kollikeri are disclosed. The amino acid sequence of R. reniformis GFP and back-translated nucleotide sequences of nucleic acids encoding the R. reniformis GFP are also disclosed. These isolated polypeptides, along with the pertinent amino acid and nucleotide sequence information, are useful in a variety of applications for which GFPs from other sources (e.g., Aequoria) are currently employed. Techniques for using the Renilla GFPs are disclosed, along with advantages of Renilla GFP as compared with currently available GFPs.

Abstract: Novel methods of detecting fluorescent proteins are described. The methods result in vastly improved signal-to-noise ratios in assays measuring fluorescence of a fluorescent protein specifically by employing a unique trapping step to microconcentrate the fluorescent protein and by using improved optical techniques. The trapping step may be a chemical or physical process or a combination thereof leading to substantial microconcentration of the fluorescent protein with concomitant removal of contaminants or interfering compounds. The methods are readily adaptable to high throughput screening and can be engineered for use with a wide variety of assays currently using microplate readers. Green fluorescent protein and fluorescent coral proteins are among preferred fluorescent proteins for the methods.