Abstract: An automatic inspection system using barcode locator and the method thereof which can inspect a colored surface of a three-dimensional object having a barcode. The automatic inspection systems comprises a light source; an image-retrieving device; and an operating and processing device. The image-retrieving device retrieves three-dimensional images of the inspected object from various viewing angles. The three-dimensional images are converted into two-dimensional images, which are then connected to produce a two-dimensional image representing the inspected object by the operating and processing device. The two-dimensional image representing the inspected object is adjusted through the barcode locator. Thereafter, the two-dimensional image is compared with a reference object image previously stored by the operating and processing device to find the defined pre-defects of the inspected object.