Abstract: The present disclosure provides methods of selectively label an amino acid residue on a peptide by replacing a post translational modification with a labeling moiety and sequencing the peptide to obtain the location of the amino acid residue and the identity of the post translational modification. In some aspects, the disclosure also provides methods of identifying the position, quantity, the identity of a post translational modification, or any combination thereof, in peptides which may be used for therapeutic purposes.

Abstract: An apparatus and method for manufacturing flexible duct comprising feeding a strip of cloth and a channel formed strip of metal in parallel through a work station having a pair of crimping rollers which fasten an edge of the fabric into the channel. A forming ring is provided at the work station to guide a first coil of fabric attached to channel strip around to meet and overlap a second coil of fabric being fed into the work station. A roller member is rotatably fixed above the work station to force the overlapping edges of adjacent coils of fabric down into the channel of the metal strip being simultaneously fed in parallel with the fabric toward the crimping rolls. The roller member carries with it a string which it inserts into the channel over the overlapping edges of the fabric prior to the combined materials being fed into crimping rollers where they are forced into a retained and fixed position within the enfolded arms of the crimped channel of metal.