Abstract: This invention relates to equilibrium dialysis systems and methods. More particularly, the invention relates to pre-assembled or ready-to-use single-well or multi-well dialysis systems that are disposable in their entirety, comprising a molded block for performing equilibrium dialysis of one or more samples. Such equilibrium dialysis systems can be used for protein binding assays, molecule-molecule interaction studies, tissue cultures and many other biological and chemical applications. Such equilibrium dialysis systems are suitable for use in manual or high throughput formats.

Abstract: The invention provides devices and methods using assemblies of shaped inserts coated with diverse binder materials optionally positioned inside conformal housings. The devices are capable of immobilizing target analytes of interest (e.g., specific or groups of biomolecules) and are optionally usable as single units, linear strips or array formats. The coatings on the inserts and, optionally also on the inside walls of the housings, create variable gaps with narrow fluid paths resulting in enhanced diffusion and absorption of target analytes from transiting fluid samples while exhibiting minimal resistance to flow or back pressure commonly seen in conventional plug-type packings.