Abstract: The invention relates to the various uses of a fungus strain which belongs to the Trichoderma genus and in which the cel1a gene has been knocked out. The invention relates in particular to a method of producing proteins by a fungus strain which belongs to the Trichoderma genus and in which the cel1a gene has been knocked out, comprising at least two steps: a first step of growth in a batch phase in the presence of at least one carbon growth substrate, and a second step of producing proteins in a fed-batch phase in the presence of a composition comprising at least lactose and a second sugar, the lactose content in the composition representing approximately between 0 and 30% by weight of the total sugar content of the composition, in particular between 5 and 30%.

Abstract: The present invention concerns a process for producing enzymes by a strain belonging to a filamentous fungus, said process comprising two steps: (a) a first step of growing the fungi, in the presence of at least one carbon-based growth substrate, in a stirred and aerated bioreactor in batch phase, at a pH of not more than 4.6; (b) a second step of producing enzymes, starting from the culture medium obtained in the first step (a), in the presence of at least one inductive carbon-based substrate, at a pH of not more than 4.6.

Abstract: The present invention relates to a method for producing enzymes by a strain belonging to a filamentous fungus, which comprises three steps: (a) a first step of growing biomass, in the presence of at least one carbon-based growth substrate in a stirred and aerated bioreactor (1) in batch phase, (b) a second step of diluting the culture medium obtained in the first step (a), (c) a third step of producing enzymes from the diluted culture medium obtained in the second step (b), in the presence of at least one inductive carbon-based substrate, in fed-batch phase.

Abstract: The invention relates to a process for producing cellulolytic or hemicellulolytic enzymes comprising A growth phase a) of a cellulolytic microorganism in a closed reactor, in the presence of at least one carbonaceous growth substrate in a concentration between 10 and 90 g/L, at a temperature of 25-30° C. and a pH of 4-5.5 An enzyme production phase b) in which at least one inducer carbonaceous substrate is added, at a temperature of 25-27° C. and a pH of 4-5, process in which the said inducer substrate is a pretreated pomace obtained from a pretreatment process of lignocellulosic material that has not undergone enzymatic hydrolysis and that is added in fed-batch or continuous mode, and which has particular characteristics: a hydrolysis yield greater than 80% in a test and an apparent viscosity, measured in the test, of less than 1 Pa·s for a shear rate of 10 s?1.

Abstract: The present invention relates to a Trichoderma reesei strain which is hyper-producing and which has enhanced ?-glucosidase activity, as well as the use of said strain.

Abstract: The present invention relates to a Trichoderma reesei strain which is hyper-producing and which has enhanced ?-glucosidase activity, as well as the use of said strain.

Abstract: The invention relates to a process for producing cellulolytic or hemicellulolytic enzymes comprising A growth phase a) of a cellulolytic microorganism in a closed reactor, in the presence of at least one carbonaceous growth substrate in a concentration between 10 and 90 g/L, at a temperature of 25-30° C. and a pH of 4-5.5 An enzyme production phase b) in which at least one inducer carbonaceous substrate is added, at a temperature of 25-27° C. and a pH of 4-5, process in which the said inducer substrate is a pretreated pomace obtained from a pretreatment process of lignocellulosic material that has not undergone enzymatic hydrolysis and that is added in fed-batch or continuous mode, and which has particular characteristics: a hydrolysis yield greater than 80% in a test and an apparent viscosity, measured in the test, of less than 1 Pa·s for a shear rate of 10 s?1.

Abstract: The invention relates to a process for the production of an enzymatic cocktail from a cellulolytic microorganism producing cellulases and/or hemi-cellulases, comprising: a step for the production of enzymes with a medium being obtained containing enzymes and a microorganism must, said must is separated, or not separated, from the liquid containing said enzymes, a step of cooling said must to a temperature below the temperature of the enzyme production step and for a time such that the beta-glucosidase concentration of the liquid originating from the cooling step is greater than that of the liquid originating from the enzyme production step, and/or the solid volume/total volume ratio is less than said ratio for the enzyme production step, and an enzymatic cocktail is obtained at the end of the cooling step.

Abstract: The invention relates to a process for the production of an enzymatic cocktail from a cellulolytic microorganism producing cellulases and/or hemi-cellulases, comprising: a step for the production of enzymes with a medium being obtained containing enzymes and a microorganism must, said must is separated, or not separated, from the liquid containing said enzymes, a step of cooling said must to a temperature below the temperature of the enzyme production step and for a time such that the beta-glucosidase concentration of the liquid originating from the cooling step is greater than that of the liquid originating from the enzyme production step, and/or the solid volume/total volume ratio is less than said ratio for the enzyme production step, and an enzymatic cocktail is obtained at the end of the cooling step.

Abstract: The present invention relates to a method of producing cellulolytic and/or hemicellulolytic enzymes by a cellulolytic microorganism in a stirred and aerated bioreactor, comprising a growth stage in the presence of a carbon source and a production stage in the presence of a carbon-containing inductive substrate, wherein the supply of carbon-containing inductive substrate during the production stage is regulated by an oscillation of the dissolved oxygen partial pressure in the medium.

Abstract: The present invention concerns a process for the production of cellulases using a strain of a filamentous fungus in a stirred, aerated bioreactor, comprising at least two growth steps: the first growth step in the presence of at least one carbonaceous growth substrate in batch phase with a concentration of carbonaceous growth substrate in the range 10 to 80 g/L; a second step for growth and production in the presence of at least one inducer carbonaceous substrate in fed batch phase in the presence of a limiting flow of carbon source in the range 50 to 140 mg per gram of cells per hour.

Abstract: The present invention relates to a method of producing cellulolytic and/or hemicellulolytic enzymes by a cellulolytic microorganism in a stirred and aerated bioreactor, comprising a growth stage in the presence of a carbon source and a production stage in the presence of a carbon-containing inductive substrate, wherein the supply of carbon-containing inductive substrate during the production stage is regulated by an oscillation of the dissolved oxygen partial pressure in the medium.