Patents by Inventor Cesario F. Bianchi

Cesario F. Bianchi has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method of differential display of exposed mRNA by RT/PCR
    Patent number: 5599672
    Abstract: A method for isolating mRNAs as cDNAs employs a polymerase amplification method using at least two oligodeoxynucleotide primers. In one approach, the first primer contains sequence capable of hybridizing to a site immediately upstream of the first A ribonucleotide of the mRNA's polyA tail and the second primer contains arbitrary sequence. In another approach, the first primer contains sequence capable of hybridizing to a site including the mRNA's polyA signal sequence and the second primer contains arbitrary sequence. In another approach, the first primer contains arbitrary sequence and the second primer contains sequence capable of hybridizing to a site including the Kozak sequence. In another approach, the first primer contains a sequence that is substantially complementary to the sequence of a mRNA having a known sequence and the second primer contains arbitrary sequence.
    Type: Grant
    Filed: December 8, 1994
    Date of Patent: February 4, 1997
    Assignees: Dana-Farber Cancer Institute, Inc., The President and Fellows of Harvard College
    Inventors: Peng Liang, Arthur B. Pardee, Cesario F. Bianchi