Patents by Inventor Chad A. Mirkin

Chad A. Mirkin has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20210163707
    Abstract: Disclosed herein are method and design rules for making polyelemental systems with specific heterostructures, including tetra-phase nanopartides with as many as six junctions.
    Type: Application
    Filed: August 16, 2019
    Publication date: June 3, 2021
    Inventors: Chad A. Mirkin, PengCheng Chen
  • Publication number: 20210122778
    Abstract: The present disclosure is directed to spherical nucleic acids (SNAs) comprising a nanoparticle core and an oligonucleotide dendron attached thereto. The disclosure also provides methods of using the SNAs for, for example, gene regulation and immune regulation.
    Type: Application
    Filed: October 21, 2020
    Publication date: April 29, 2021
    Inventors: Chad A. Mirkin, Katherine E. Bujold, Max E. Distler, Caroline Kusmierz
  • Publication number: 20210123057
    Abstract: The present disclosure is directed to spherical nucleic acids (SNAs) comprising a nanoparticle core and an oligonucleotide shell attached to the external surface of the nanoparticle core, wherein the oligonucleotide shell comprises a mixture of class A CpG oligonucleotides and class B CpG oligonucleotides. The disclosure also provides methods of using the SNAs for, e.g., regulation of an immune response and gene regulation.
    Type: Application
    Filed: October 29, 2020
    Publication date: April 29, 2021
    Inventors: Chad A. Mirkin, Ziyin Huang
  • Publication number: 20210114288
    Abstract: Methods and apparatus comprising a dewetting phase and a polymerization liquid that are immiscible, and can be used for the formation of three-dimensional objects, wherein the method does not require a dead zone. Additionally, methods and apparatus that employ an optically transparent cooling apparatus to mitigate heat generated during the fabrication process, and the use of a mobile phase to provide a shearing interface to reduce interfacial adhesive forces.
    Type: Application
    Filed: October 30, 2020
    Publication date: April 22, 2021
    Inventors: Chad A. Mirkin, David A. Walker, James L. Hedrick, III
  • Publication number: 20210087221
    Abstract: The disclosure is generally related to spherical nucleic acids (SNAs) comprising a protein corona, wherein the SNA comprises (i) a nanoparticle core and (ii) one or more oligonucleotides attached to the surface of the nanoparticle core, wherein the protein corona comprises a plurality of proteins. The disclosure also provides methods of using the same. The disclosure further provides methods of improving stability and/or extending blood circulation half-life of a spherical nucleic acid (SNA), the SNA comprising a nanoparticle core and one or more oligonucleotides attached to the surface of the nanoparticle core, the method comprising adsorbing a plurality of proteins on the surface of the SNA.
    Type: Application
    Filed: September 18, 2020
    Publication date: March 25, 2021
    Inventors: Chad A. Mirkin, Wuliang Zhang, Brian R. Meckes
  • Publication number: 20210055455
    Abstract: Provided herein are methods of preparing three-dimensional photonic crystals having tunable optical properties and control over stopband location and width, the three-dimensional photonic crystals comprising nanoparticles and spacer groups.
    Type: Application
    Filed: January 31, 2019
    Publication date: February 25, 2021
    Inventors: Chad A. Mirkin, Lin Sun, Haixin Lin, George C. Schatz
  • Publication number: 20210052497
    Abstract: Liposomes termed as small unilamellar vesicles (SUVs), can be synthesized in the 20-50 nm size range, but encounter challenges such as instability and aggregation leading to inter-particle fusion. This limits their use as a therapeutic delivery agent. Increasing the surface negative charge of SUVs, via the attachment of anionic entities such as DNA/RNA, increases the colloidal stability of these vesicles. Additionally, the dense spherical arrangement and radial orientation of nucleic acids exhibits unique chemical and biological properties, unlike their linear counterparts. These liposomal particles, are non-toxic and though anionic, can efficiently enter cells without the aid of ancillary cationic transfection agents in a non-immunogenic fashion. These exceptional properties allow their use as delivery agents for gene regulation in different therapies and offer an alternative platform to metal core spherical nucleic acids.
    Type: Application
    Filed: September 3, 2020
    Publication date: February 25, 2021
    Inventors: Chad A. Mirkin, Sonbinh T. Nguyen, Resham Singh Banga, Natalia Chernyak, Sergei Gryaznov, Aleksandar Radovic-Moreno, Christopher Mader
  • Publication number: 20210039062
    Abstract: The present disclosure relates to nanolithographical cell patterning. In some aspects, the present disclosure provides materials and methods for making an oriented array.
    Type: Application
    Filed: February 27, 2019
    Publication date: February 11, 2021
    Inventors: Chad A. Mirkin, Milan Mrksich, Brian R. Meckes, Maria D. Cabezas
  • Publication number: 20200407812
    Abstract: Disclosed herein are methods for forming one or more nanoparticles. The methods include depositing a solution comprising a block copolymer and a metal salt into one or more square pyramidal nanoholes formed in a substrate, and annealing the substrate to provide a single nanoparticle in each of the one or more square pyramidal nanoholes.
    Type: Application
    Filed: June 26, 2020
    Publication date: December 31, 2020
    Inventors: Chad A. Mirkin, Liban Jibril, Pengcheng Chen
  • Publication number: 20200399304
    Abstract: Disclosed herein are spherical nucleic acids (SNAs) comprising oligonucleotides comprising one or more modified oligonucleotides, and methods of use thereof. Also disclosed are methods of synthesizing modified oligonucleotides for use in therapeutics, including deoxynucleic guanidine (DNG)-modified oligonucleotides.
    Type: Application
    Filed: June 19, 2020
    Publication date: December 24, 2020
    Inventors: Chad A. Mirkin, Kacper Skakuj, Katherine E. Bujold
  • Publication number: 20200384104
    Abstract: The present disclosure provides compositions and methods comprising spherical nucleic acid (SNA) components for use as immunotherapeutic agents. The disclosure provides a method comprising: treating a population of antigen presenting cells with a SNA comprising a nanoparticle, an antigen, and an adjuvant; and determining a time at which the population of antigen presenting cells presents a maximal signal that is indicative of antigen presentation by the antigen presenting cells and a time at which the population of antigen presenting cells presents a maximal co-stimulatory signal due to the adjuvant. The disclosure includes compositions that comprise a pharmaceutically acceptable carrier and a SNA of the disclosure, wherein the SNA comprises a nanoparticle, an oligonucleotide on the surface of the nanoparticle, and an antigen that is associated with the surface of the SNA via a linker. The disclosure additionally includes articles of manufacture and kits.
    Type: Application
    Filed: December 14, 2018
    Publication date: December 10, 2020
    Inventors: Chad A. Mirkin, Shuya Wang, Bin Zhang, Kacper Skakuj
  • Publication number: 20200368708
    Abstract: Methods of stabilizing DNA-engineered crystals can include cross-linking the hybridized oligonucleotides. Stabilized crystals can have improved chemical and thermal stability.
    Type: Application
    Filed: May 22, 2020
    Publication date: November 26, 2020
    Inventors: Chad A. Mirkin, Seungkyu Lee, Cindy Yizhe Zheng, Katherine E. Bujold
  • Patent number: 10792251
    Abstract: Liposomes termed as small unilamellar vesicles (SUVs), can be synthesized in the 20-50 nm size range, but encounter challenges such as instability and aggregation leading to inter-particle fusion. This limits their use as a therapeutic delivery agent. Increasing the surface negative charge of SUVs, via the attachment of anionic entities such as DNA/RNA, increases the colloidal stability of these vesicles. Additionally, the dense spherical arrangement and radial orientation of nucleic acids exhibits unique chemical and biological properties, unlike their linear counterparts. These liposomal particles, are non-toxic and though anionic, can efficiently enter cells without the aid of ancillary cationic transfection agents in a non-immunogenic fashion. These exceptional properties allow their use as delivery agents for gene regulation in different therapies and offer an alternative platform to metal core spherical nucleic acids.
    Type: Grant
    Filed: January 8, 2019
    Date of Patent: October 6, 2020
    Assignees: NORTHWESTERN UNIVERSITY, EXICURE, INC.
    Inventors: Chad A. Mirkin, Sonbinh T. Nguyen, Resham Singh Banga, Natalia Chernyak, Sergei Gryaznov, Aleksandar Radovic-Moreno, Christopher Mader
  • Publication number: 20200310248
    Abstract: A method of preparing a metal nanoparticle can include depositing an ink on a substrate using nanolithography to form a polymer reactor, wherein the ink comprises at least one metalloporphyrin functionalized polymer comprising a metal ion, porphyrin, and a polyethylene oxide polymer; and thermally annealing the polymer nanoreactor under conditions sufficient to form the nanoparticle. The thermal annealing can include a first stage that includes annealing the polymer nanoreactor at a temperature of about 100° C. to about 350° C. for a time sufficient to aggregate the metal ions in the polymer reactor, and a second stage that includes annealing the aggregated polymer nanoreactor at a temperature of about 500° C. to about 600° C. for a time sufficient to reduce the metal ion and decompose a polymer component of the polymer nanoreactor to thereby form the metal nanoparticle.
    Type: Application
    Filed: March 27, 2020
    Publication date: October 1, 2020
    Inventors: Chad A. Mirkin, Pengcheng Chen, Yuan Liu, Jingshan Du
  • Publication number: 20200291394
    Abstract: The present disclosure provides compositions and methods directed to combining spherical nucleic acid (SNA) components that are required for T-cell activation and proliferation.
    Type: Application
    Filed: May 17, 2018
    Publication date: September 17, 2020
    Applicant: Northwestern University
    Inventors: Chad A. Mirkin, Kacper Skakuj, Shuya Wang
  • Publication number: 20200246484
    Abstract: The present disclosure provides compositions and methods directed to the synthesis and use of SNAs with a sheddable PEG layer that incorporates an enzyme-sensitive linker.
    Type: Application
    Filed: October 3, 2018
    Publication date: August 6, 2020
    Inventors: Chad A. Mirkin, Brian R. Meckes, Wuliang Zhang
  • Publication number: 20200230581
    Abstract: Disclosed herein are trimetallic PtAu-based nanocatalysts for electrochemical hydrogen production and screening methods thereof. Nanocatalysts are produced through a polymer pen lithography (PPL) technique, which enables large-scale fabrication of nanoparticle arrays with programmable specifications such as size, shape, and composition, providing a route to the high-throughput screening and discovery of new catalysts.
    Type: Application
    Filed: October 3, 2018
    Publication date: July 23, 2020
    Inventors: Chad A. Mirkin, Yijin Kang, Liliang Huang
  • Publication number: 20200101156
    Abstract: The disclosure is related to compositions comprising a cell and a spherical nucleic acid (SNA) comprising a nanoparticle, an oligonucleotide on the surface of the nanoparticle, and an antigen; and to methods for production of such compositions and their applications, including but not limited to adoptive cell therapy.
    Type: Application
    Filed: May 11, 2018
    Publication date: April 2, 2020
    Applicant: Northwestern University
    Inventors: Chad A. Mirkin, Andrew Lee, Bin Zhang, Donye Dominguez
  • Patent number: 10563244
    Abstract: The present disclosure provides methods in which adherent cells are treated with small molecules, cultured, lysed, and then analyzed by mass spectrometry to measure the activities of endogenous enzymes. The implementation of this method relies on the use of surfaces that are nanopatterned with cell adhesion ligands to mediate cell attachment and a peptide that is a substrate for the desired enzyme activity in the lysate.
    Type: Grant
    Filed: September 20, 2017
    Date of Patent: February 18, 2020
    Assignee: NORTHWESTERN UNIVERSITY
    Inventors: Milan Mrksich, Maria D. Cebezas, Chad A. Mirkin
  • Publication number: 20200022913
    Abstract: Liposomes termed as small unilamellar vesicles (SUVs), can be synthesized in the 20-50 nm size range, but encounter challenges such as instability and aggregation leading to inter-particle fusion. This limits their use as a therapeutic delivery agent. Increasing the surface negative charge of SUVs, via the attachment of anionic entities such as DNA/RNA, increases the colloidal stability of these vesicles. Additionally, the dense spherical arrangement and radial orientation of nucleic acids exhibits unique chemical and biological properties, unlike their linear counterparts. These liposomal particles, are non-toxic and though anionic, can efficiently enter cells without the aid of ancillary cationic transfection agents in a non-immunogenic fashion. These exceptional properties allow their use as delivery agents for gene regulation in different therapies and offer an alternative platform to metal core spherical nucleic acids.
    Type: Application
    Filed: January 8, 2019
    Publication date: January 23, 2020
    Inventors: Chad A. Mirkin, Sonbinh T. Nguyen, Resham Singh Banga, Natalia Chernyak, Sergei Gryaznov, Aleksandar Radovic-Moreno, Christopher Mader