Patents by Inventor Chad FLEISCHER

Chad FLEISCHER has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11339428
    Abstract: A method for identifying a nucleotide in a template nucleic acid by (a) providing a plurality of primer-template nucleic acid hybrids, wherein the primers have an extendable 3? end; (b) contacting the plurality with: (i) blocked nucleotides to produce a first subset of the primer-template nucleic acid hybrids that include a blocked nucleotide at the 3? end, and (ii) a ternary complex inhibitor to produce a second subset of the primer-template nucleic acid hybrids that include a ternary complex inhibitor; (c) forming ternary complexes that each include a polymerase, a primer-template nucleic acid hybrid of the first subset, and a cognate nucleotide; and (d) detecting the ternary complexes, thereby identifying a nucleotide in the template nucleic acid.
    Type: Grant
    Filed: April 12, 2019
    Date of Patent: May 24, 2022
    Assignee: PACIFIC BIOSCIENCES OF CALIFORNIA, INC.
    Inventors: Chad Fleischer, Denis Malyshev
  • Publication number: 20220106628
    Abstract: A method for identifying a nucleic acid template that include (a) providing a plurality of primer-template hybrids, wherein a first hybrid of the plurality includes a first template hybridized to a first primer, and wherein a second hybrid of the plurality includes a second template hybridized to a second primer, the second primer having a ternary complex inhibitor moiety at the 3? end; (b) delivering polymerases and nucleotides to the plurality, whereby the first hybrid binds a polymerase and nucleotide to form a stabilized ternary complex and whereby the second hybrid does not bind a polymerase and nucleotide to form a stabilized ternary complex; and (c) detecting the stabilized ternary complex to identify the first template.
    Type: Application
    Filed: October 15, 2021
    Publication date: April 7, 2022
    Applicant: Omniome, Inc.
    Inventors: Hari K.K. SUBRAMANIAN, Chad FLEISCHER, Denis MALYSHEV
  • Patent number: 11180794
    Abstract: A method for identifying a nucleic acid template that includes (a) providing a plurality of primer-template hybrids, wherein a first hybrid of the plurality includes a first template hybridized to a first primer, and wherein a second hybrid of the plurality includes a second template hybridized to a second primer, the second primer having a ternary complex inhibitor moiety at the 3? end; (b) delivering polymerases and nucleotides to the plurality, whereby the first hybrid binds a polymerase and nucleotide to form a stabilized ternary complex and whereby the second hybrid does not bind a polymerase and nucleotide to form a stabilized ternary complex; and (c) detecting the stabilized ternary complex to identify the first template.
    Type: Grant
    Filed: October 18, 2019
    Date of Patent: November 23, 2021
    Assignee: OMNIOME, INC.
    Inventors: Hari K. K. Subramanian, Chad Fleischer, Denis Malyshev
  • Publication number: 20200290047
    Abstract: An analytical system that includes a flow cell, a liquid delivery component, a gas delivery component and a bubble generator component, wherein the liquid delivery component is configured to deliver liquid from one or more reservoirs to the bubble generator component, wherein the gas delivery component is configured to deliver gas from one or more source to the bubble generator component, and wherein the bubble generator component is configured to mix liquids from the liquid delivery component with gas from the gas delivery component to deliver a fluid foam to the inside of the flow cell, wherein the fluid foam includes bubbles of the gas in the liquid.
    Type: Application
    Filed: June 2, 2020
    Publication date: September 17, 2020
    Applicant: OMNIOME, INC.
    Inventors: Arnold OLIPHANT, Alex NEMIROSKI, Julian Sean ALBERNI, Michael John ERICKSTAD, Rebecca MCGINLEY, Chad FLEISCHER, Eric VILLARREAL
  • Patent number: 10710076
    Abstract: An analytical system that includes a flow cell, a liquid delivery component, a gas delivery component and a bubble generator component, wherein the liquid delivery component is configured to deliver liquid from one or more reservoirs to the bubble generator component, wherein the gas delivery component is configured to deliver gas from one or more source to the bubble generator component, and wherein the bubble generator component is configured to mix liquids from the liquid delivery component with gas from the gas delivery component to deliver a fluid foam to the inside of the flow cell, wherein the fluid foam includes bubbles of the gas in the liquid.
    Type: Grant
    Filed: December 2, 2019
    Date of Patent: July 14, 2020
    Assignee: OMNIOME, INC.
    Inventors: Arnold Oliphant, Alex Nemiroski, Julian Sean Alberni, Michael John Erickstad, Rebecca McGinley, Chad Fleischer, Eric Villarreal
  • Publication number: 20200171498
    Abstract: An analytical system that includes a flow cell, a liquid delivery component, a gas delivery component and a bubble generator component, wherein the liquid delivery component is configured to deliver liquid from one or more reservoirs to the bubble generator component, wherein the gas delivery component is configured to deliver gas from one or more source to the bubble generator component, and wherein the bubble generator component is configured to mix liquids from the liquid delivery component with gas from the gas delivery component to deliver a fluid foam to the inside of the flow cell, wherein the fluid foam includes bubbles of the gas in the liquid.
    Type: Application
    Filed: December 2, 2019
    Publication date: June 4, 2020
    Applicant: OMNIOME, INC.
    Inventors: Arnold OLIPHANT, Alex NEMIROSKI, Julian Sean ALBERNI, Michael John ERICKSTAD, Rebecca MCGINLEY, Chad FLEISCHER, Eric VILLARREAL
  • Publication number: 20200032322
    Abstract: A method for identifying a nucleic acid template that include (a) providing a plurality of primer-template hybrids, wherein a first hybrid of the plurality includes a first template hybridized to a first primer, and wherein a second hybrid of the plurality includes a second template hybridized to a second primer, the second primer having a ternary complex inhibitor moiety at the 3? end; (b) delivering polymerases and nucleotides to the plurality, whereby the first hybrid binds a polymerase and nucleotide to form a stabilized ternary complex and whereby the second hybrid does not bind a polymerase and nucleotide to form a stabilized ternary complex; and (c) detecting the stabilized ternary complex to identify the first template.
    Type: Application
    Filed: October 18, 2019
    Publication date: January 30, 2020
    Applicant: Omniome, Inc.
    Inventors: Hari SUBRAMANIAN, Chad FLEISCHER, Denis MALYSHEV
  • Publication number: 20190367974
    Abstract: A method for identifying a nucleotide in a template nucleic acid by (a) providing a plurality of primer-template nucleic acid hybrids, wherein the primers have an extendable 3? end; (b) contacting the plurality with: (i) blocked nucleotides to produce a first subset of the primer-template nucleic acid hybrids that include a blocked nucleotide at the 3? end, and (ii) a ternary complex inhibitor to produce a second subset of the primer-template nucleic acid hybrids that include a ternary complex inhibitor; (c) forming ternary complexes that each include a polymerase, a primer-template nucleic acid hybrid of the first subset, and a cognate nucleotide; and (d) detecting the ternary complexes, thereby identifying a nucleotide in the template nucleic acid.
    Type: Application
    Filed: April 12, 2019
    Publication date: December 5, 2019
    Applicant: Omniome, Inc.
    Inventors: Chad FLEISCHER, Denis MALYSHEV