Abstract: A guide for use with a manual pipette used in loading well plates is provided which insures the accuracy and efficiency of reservoir loading. The pipette guide is used in combination with a conventional well plate generally having 96-384 reservoirs. The guide allows for more exact delivery of liquid reagents to selected reservoirs without concern for missing or duplicating particular reservoirs during loading. The pipette guide is releasably locked onto the well plate and a slide outlines a specific row of reservoirs. In addition a reservoir isolator allows delivery to a single selected reservoir and protects against cross-contamination. Adjustable rods with notches therealong allow the slide and isolator to be precisely moved and indexed as desired.