Abstract: Embodiments disclosed herein are directed to bioreactor devices and systems that allow cultured cells to grow and develop with a three-dimensional aspect. In addition, the bioreactor systems described herein can be used for variation and independent control of environmental factors within the individual sub-wells which can be advantageously co-located in a common chamber. For example, the chemical make-up of a nutrient medium that can flow through a chamber as well as the mechanical force environment within the chamber, including the perfusion flow, shear stress, hydrostatic pressure, and the like, can be independently controlled and maintained for each separate culture chamber of the disclosed systems. Further, the bioreactor systems are designed for easy incorporation into automated systems and minimize or eliminate tubing.