Abstract: The present invention relates to a heat stress resisting technology for transport of live fish. Display screens are arranged in a transport case on the four side surfaces and the top surface, a plurality of transparent interlayer trays are arranged in the fish tank from the bottom to the top, a plurality of small through holes are formed in the interlayer trays, an oxygen generator is arranged in the transport case, an oxygen content meter is arranged in a pipeline of the oxygen generator, a refrigeration system is arranged in the transport case on one side surface, an air exchange pipe is arranged in the fish tank, the air exchange pipe extends out of the transport case, a second solenoid valve is arranged in the air exchange pipe. The present invention makes fish have no swimming activity and lack of response to external stimuli.

Abstract: The invention discloses a modified chitosan-LZM-CA composite antibacterial cling film optimized by response surface methodology and the preparation method and application thereof, relates to the technical field of food packaging materials. The invention provides freshwater crayfish as the research object, and adopts the modified chitosan, lysozyme (LZM), a good film-forming material carrageenan (CA) as the composite antibacterial cling film materials, and through the response surface experimental design to determine the best ratio of modified chitosan-LZM-CA composite antibacterial cling film. Furthermore, the TVB-N value and the change value of the total number of bacterial colonies of the freshwater crayfish with the composite antibacterial cling film during refrigeration, can be regarded as indicators to examine the preservation effect, providing a theoretical basis of the chitosan composite film preservation technology development and application for freshwater crayfish and other aquatic products.