Abstract: The subject disclosure relates in part to endpoint TaqMan® PCR assays for the detection and high throughput zygosity analysis of the fad-3c gene in canola. The subject disclosure further relates, in part, to the use of wild type DNA as a reference for use in determining zygosity. These and other related procedures can be used to uniquely identify the zygosity and variety of canola lines comprising the subject gene. The subject disclosure also provides related kits for determining zygosity from a sample of a canola plant or seed, for example.

Abstract: The subject disclosure relates in part to endpoint TaqMan® PCR assays for the detection and high throughput zygosity analysis of the fad-3c gene in canola. The subject disclosure further relates, in part, to the use of wild type DNA as a reference for use in determining zygosity. These and other related procedures can be used to uniquely identify the zygosity and variety of canola lines comprising the subject gene. The subject disclosure also provides related kits for determining zygosity from a sample of a canola plant or seed, for example.

Abstract: The subject disclosure relates in part to endpoint TaqMan® PCR assays for the detection and high throughput zygosity analysis of the fad-3c gene in canola. The subject disclosure further relates, in part, to the use of wild type DNA as a reference for use in determining zygosity. These and other related procedures can be used to uniquely identify the zygosity and variety of canola lines comprising the subject gene. The subject disclosure also provides related kits for determining zygosity from a sample of a canola plant or seed, for example.

Abstract: This application provides materials and methods for the detection of the aad-12 gene and event pDAB4472-1606 in biological samples derived from plants.

Abstract: This disclosure concerns compositions and methods for determining the zygosity of corn plants containing one or more brown midrib (BMR) mutations. The disclosure also concerns methods that are useful for enhancing the breeding process for BMR corn. In certain embodiments, compositions and methods for determining the zygosity of corn plants with respect to the bm3 allele.

Abstract: This application provides materials and methods for the detection of aad-12 gene events in biological samples derived from recombinant plants and a materials and methods for the detection of contaminating events in samples derived from recombinant plants.

Abstract: Methods of determining the presence or absence of an inserted nucleotide sequence at a particular insertion site in a nucleic acid include: isolating a nucleic acid from the bulked tissue sample; contacting the nucleic acid with a forward primer able to bind to the nucleic acid upstream of the insertion site, a first reverse primer specific for the inserted nucleotide sequence, and a second reverse primer able to bind to the nucleic acid downstream of the insertion site. The primers may be used to reproduce nucleic acids between the primers. The reproduced nucleic acids may be analyzed to determine if an inserted nucleotide sequence is present or absent in the sample.

Abstract: The present disclosure provides systems and methods for normalization of multiple samples, including DNA samples. The subject invention allows for decreased human error, decreased ergonomic concerns, and an increase in throughput. The subject invention provides systems and methods that allow for automated normalization of samples in multi-well plates. According to the present disclosure, a method for normalizing samples is provided. Some preferred methods comprise determining florescence data of one or more wells on a plate; electronically, using a processor, calculating dilution data for one or more of the wells on the plate based at least in part on the florescence data; and adding a liquid to the one or more wells on the plate based at least in part on the dilution data.

Abstract: This disclosure concerns compositions and methods for determining the zygosity of corn plants containing one or more brown midrib (BMR) mutations. The disclosure also concerns methods that are useful for enhancing the breeding process for BMR corn. In certain embodiments, compositions and methods for determining the zygosity of corn plants with respect to the bm3 allele.