Abstract: The present invention relates to a method capable of, in order to diagnose fetal sex chromosome aneuploidy, differentiating Kleinfeiter's syndrome (XXY), triple X syndrome (XXX), and Turner's syndrome (monosomy X, XO) as well as male (XY) and female (XX) by using copy number variation (CNV). The differentiation method according to the present invention has significantly high sensitivity and accuracy since the reference line is evenly adjusted by performing normalization regardless of the kinds of platform and data. The present invention is useful in diagnosing the sex chromosome abnormality at an early stage through easy diagnosis of sex chromosomes X and Y, which are hard to diagnose, since an analysis is possible even with a small amount of fetal chromosomes, which corresponds to an advantage of noninvasive prenatal diagnosis, and copies are redundant.

Abstract: A colored coin having an individual gene phenotype applied thereto. Particularly, a colored coin manufactured and having an individual gene phenotype applied thereto such that an individual can be identified, cannot only be circulated freely as a Bitcoin block or colored coin metadata, but also is useful in various fields, such as gene-based character fabrication and gaming.

Abstract: The present invention relates to a method capable of, in order to diagnose fetal sex chromosome aneuploidy, differentiating Kleinfeiter's syndrome (XXY), triple X syndrome (XXX), and Turner's syndrome (monosomy X, XO) as well as male (XY) and female (XX) by using copy number variation (CNV). The differentiation method according to the present invention has significantly high sensitivity and accuracy since the reference line is evenly adjusted by performing normalization regardless of the kinds of platform and data. The present invention is useful in diagnosing the sex chromosome abnormality at an early stage through easy diagnosis of sex chromosomes X and Y, which are hard to diagnose, since an analysis is possible even with a small amount of fetal chromosomes, which corresponds to an advantage of noninvasive prenatal diagnosis, and copies are redundant.

Abstract: A Method is provided for determining chromosome abnormalities. The method includes sequencing next-generation sequencing (NGS) sequence data regardless of an NGS analysis platform, determining male or female by extracting a unique-read from the sequenced sequence data, and setting a threshold line using initial learning by linear discriminant analysis (LDA) of existing data, thereby being applied for both autosomes and sex-chromosomes, and improving accuracy and sensitivity as the number of diagnoses increases.

Abstract: The present invention relates to a novel gene coding human epidermal growth factor("hEGF") and a process for preparing the same employing a recombinant expression vector therefor. The hEGF gene of the invention is designed to contain codons ubiquitous in E. coli and the following restriction sites: HpaI at the 5' terminal, PstI at the 3' terminal and Bpu1102I, NsiI, MluI, Eco47III and AflII at a regular manner within its internal sequence. The present invention also provides a process for preparing hEGF by employing a expression vector pTE105 for hEGF, which contains expression cassette comprising Omp A leader sequence, translation termination sequence and transcription termination sequence and hEGF gene; and, replication origin of pUC19, tetracycline-resistant marker and a par site for stabilization in E. coli. The hEGF is produced massively in E. coli transformed with the pTE105(KCCM 10027).