Abstract: An eyelid opener and an eyelid opening and sensing device are provided. The eyelid opener has a housing, two eyelid pushers and one eye positioning unit. The eye positioning unit abuts the surrounding of the eye socket for positioning (e.g. one side of the nasal bridge), and then the sensing process (e.g. eye pressure, eye temperature) of an eyeball sensor is started, ensuring the inspected region remains the same for each measurement. By contacting the user's face, the eyelid opener can be operated by the user independently. The positioning process ensures the eyeball sensor and the eyeball are placed at a fixed distance to prevent the eyeball sensor from physical contacting or hurting the eyeball and to facilitate mounting the eyeball sensor near the eyeball. The two eyelid pushers can avoid unconscious blinking during the sensing process.