Abstract: The present disclosure discloses a microfluidic chip, a system and a method for automatic separation and detection of circulating tumor cells. The microfluidic chip includes a density gradient centrifugation assembly, a cell capture assembly, and a reagent storage assembly. The density gradient centrifugation assembly is configured to carry out density gradient centrifugation on a whole blood sample to achieve separation and obtain a plasma layer, a monocyte layer, a Ficoll solution layer, and an erythrocyte and granulocyte layer. The cell capture assembly includes a capture channel, a capture inlet, and a capture outlet. The capture inlet and the capture outlet are in communication with the capture channel. An inner wall of the capture channel is provided with capture holes and negative-pressure capture chambers. Each capture hole is in communication with one negative-pressure capture chamber correspondingly.

Abstract: An air valve structure arranged on a base comprises an air plug and a state-switching component. The air plug is arranged in an air chamber in an axial direction. The air plug includes a closing state to close the air hole, and an opening state to open the air hole. The state-switching component comprises a driving member that links the air plug, a shape-memory alloy (SMA) wire connected with the driving member, and at least one conductive member connected with the SMA wire. The driving member exerts an acting force to the air plug based on a condition of electricity provided by the conductive member for the SMA wire. The direction of the acting force is non-parallel with the axial direction and the air plug is moved and changed between the closing state and the opening state by the acting force.

Abstract: An air valve structure arranged on a base comprises an air plug and a state-switching component. The air plug is arranged in an air chamber in an axial direction. The air plug includes a closing state to close the air hole, and an opening state to open the air hole. The state-switching component comprises a driving member that links the air plug, a shape-memory alloy (SMA) wire connected with the driving member, and at least one conductive member connected with the SMA wire. The driving member exerts an acting force to the air plug based on a condition of electricity provided by the conductive member for the SMA wire. The direction of the acting force is non-parallel with the axial direction and the air plug is moved and changed between the closing state and the opening state by the acting force.

Abstract: An air valve comprises a casing, a valve body driving assembly and a valve body. The casing comprises a chamber, a vent hole, and at least one switching hole communicating with the chamber. The valve body driving assembly is disposed in the chamber, and comprises a pusher, a shape memory metal wire in a linkage relationship with the pusher, and two conductive members disposed in the casing and located at two ends of the shape memory metal wire respectively, the pusher travels a uniaxial displacement stroke due to a temporary change of the shape memory metal wire. The valve body is disposed in the chamber and faces the switching hole, when the pusher travels the uniaxial displacement stroke, the valve body is driven to rotate a central angle relative to the switching hole to change a ventilation state between the switching hole and the chamber.

Abstract: A network element access method includes: sending, by a network element attempting to access a network, an access data request to a network element that has accessed the network, so that the network element that has accessed the network forwards the access data request to a network management element; receiving, by the network element attempting to access the network, an access data response sent by the network element that has accessed the network, where the access data response is generated by the network management element by performing authentication, and the access data response is sent, to the network element attempting to access the network, by the network management element via the network element that has accessed the network; and if the access data response indicates that the network element attempting to access the network is allowed to access the network, accessing the network.