Publication number: 20230346896
Abstract: Provided is a method for preparing a polyethylene glycol-modified urate oxidase, at least 11 of the following amino acid sites of the polyethylene glycol-modified urate oxidase have a PEG modification: T1, K3, K4, K30, K35, K76, K79, K97, K112, K116, K120, K152, K179, K222, K231, K266, K272, K285, K291, and K293, and the preparation method includes: performing a coupling reaction between urate oxidase and polyethylene glycol, wherein the polyethylene glycol is provided in the form of an acidic solution, and a molar ratio of the urate oxidase to the polyethylene glycol is 1: (56 to 94), to obtain the polyethylene glycol-modified urate oxidase.
Type:
Application
Filed:
May 4, 2023
Publication date:
November 2, 2023
Applicant:
HANGZHOU GRAND BIOLOGIC PHARMACEUTICAL INC.
Inventors:
RIYONG LIU, Zhiming WANG, Yunfeng HE, Yu WANG, Zhicheng FU, Tianwen YAN, Chunlan HU, Guowei SU, Changcheng TAN, Xupeng DING, Hui YANG, Hongying WANG, Qiong DING, Qian WANG, Haiyan WEN, Kai FAN
Publication number: 20220054598
Abstract: The present disclosure provides a polyethylene glycol-modified urate oxidase. At least 11 of the following amino acid sites in the urate oxidase have a PEG modification: T1, K3, K4, K30, K35, K76, K79, K97, K112, K116, K120, K152, K179, K222, K231, K266, K272, K285, K291, and K293.
Type:
Application
Filed:
November 9, 2021
Publication date:
February 24, 2022
Inventors:
Kai FAN, Zhiming WANG, Riyong LIU, Yu WANG, Yunfeng HE, Tianwen YAN, Zhicheng FU, Guowei SU, Chunlan HU, Xupeng DING, Changcheng TAN, Hongying WANG, Hui YANG, Qiong DING, Haiyan WEN
