Abstract: A cell model for in vitro evaluation of compound-induced skin sensitization and a constructing method therefor. The method for constructing the cell model comprises the steps of: designing and constructing an sgRNA expression vector based on CRISPR/Cas9 vector system; designing and constructing a homologous recombinant vector capable of knocking a reporter gene linked to a self-cleaving peptide sequence into a specific site of the expression frame of the HMOX1 gene; co-transfecting the homologous recombinant vector, an hCas9 plasmid and the sgRNA expression vector into a cell, and carrying out monoclonal expansion to obtain the cell model. The present invention obtains a HaCaT cell model in which a luciferase gene is knocked in before the stop codon of the HMOX1 gene by combination of CRISPR/CAS9 and a monoclonal cell technique. The cell model realizes synchronous expression of the luciferase gene and the HMOX1 gene, thereby effectively distinguishing sensitizing compounds from non-sensitizing compounds.

Abstract: Provided are an electrode assembly, a battery cell, a battery, and an electrical device. The electrode assembly includes first electrode plates and a second electrode plate that are of opposite polarities. The second electrode plate and two first electrode plates are stacked and wound to form the electrode assembly. The second electrode plate is located between the two first electrode plates. Each of the first electrode plates includes a first composite current collector and a first active material layer. The first active material layer is disposed on a surface that is of the first composite current collector and that is oriented back from the second electrode plate. The first composite current collector is configured to dielectrically isolate the first active material layer from the second electrode plate and allow passage of ions transmitted between the first active material layer and the second electrode plate.

Abstract: An electrode plate, an electrode assembly, and a secondary battery are provided. The electrode plate includes a current collector, an active material layer arranged on one surface of the current collector, and an electrical connection member electrically connected to the current collector. The active material layer is arranged on a main body portion of the current collector, the electrical connection member and the current collector are connected to each other by welding at an edge of the current collector, the welding connection region is referred to as a transfer welding region, and the current collector includes a support layer and an electrically conductive layer arranged on one surface of the support layer. The electrode plate further includes a first insulation layer arranged on a further surface of the current collector and covering at least the entirety of the transfer welding region when viewed in a thickness direction of the electrode plate.

Abstract: Provided are an electrode assembly, a battery cell, a battery, and an electrical device. The electrode assembly includes first electrode plates and a second electrode plate that are of opposite polarities. The second electrode plate and two first electrode plates are stacked and wound to form the electrode assembly. The second electrode plate is located between the two first electrode plates. Each of the first electrode plates includes a first composite current collector and a first active material layer. The first active material layer is disposed on a surface that is of the first composite current collector and that is oriented back from the second electrode plate. The first composite current collector is configured to dielectrically isolate the first active material layer from the second electrode plate and allow passage of ions transmitted between the first active material layer and the second electrode plate.

Abstract: An electrode plate, a battery cell, a battery and an electrical device are provided. The electrode plate includes an insulating substrate, a conductive layer, and an active material layer. The conductive layer is arranged on the surface of the insulating substrate. The active material layer is applied on the surface of the conductive layer away from the insulating substrate. The conductive layer includes a first part coated with the active material layer and a second part not coated with the active material layer. The first part and the second part are arranged along the first direction. The conductive layer has a resistivity of ?1, a specific heat capacity of C, a density of ?2, and a constant K=?1/(C·?2). The conductive layer has a thickness of d1, the first part has a size of W along the first direction, and d1, W and K satisfy: 0.001 J/(?·mm4·° C.)?d1/(K·W)?0.0075 J/(?·mm4·° C.).

Abstract: A cell model for in vitro evaluation of compound-induced skin sensitization and a constructing method therefor. The method for constructing the cell model comprises the steps of: designing and constructing an sgRNA expression vector based on CRISPR/Cas9 vector system; designing and constructing a homologous recombinant vector capable of knocking a reporter gene linked to a self-cleaving peptide sequence into a specific site of the expression frame of the HMOX1 gene; co-transfecting the homologous recombinant vector, an hCas9 plasmid and the sgRNA expression vector into a cell, and carrying out monoclonal expansion to obtain the cell model. The present invention obtains a HaCaT cell model in which a luciferase gene is knocked in before the stop codon of the HMOX1 gene by combination of CRISPR/CAS9 and a monoclonal cell technique. The cell model realizes synchronous expression of the luciferase gene and the HMOX1 gene, thereby effectively distinguishing sensitizing compounds from non-sensitizing compounds.