Abstract: A physiological detection method includes the following steps. A detection portion of a human body is detected to obtain a detection signal. Then, the detection signal is processed to output a digital physiological signal. The digital physiological signal is received to calculate and obtain first information and second information related to feature points thereof. Then, a ratio of the second information to the first information is calculated to obtain a physiological condition index. The digital physiological signal includes pulse waves generated according to a time sequence. The feature points of the digital physiological signal include a wave pulse peak and a foot point located at a forepart of the rising edge of the wave. In addition, a physiological detection device is also introduced.