Patents by Inventor Chao-Ling Yao

Chao-Ling Yao has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 8762074
    Abstract: The present invention provides a method of determining the optimal composition of a serum-free, eukaryotic cell culture medium supplement, using 2-level factorial design and the steepest ascent method. The invention further provides a method of making a serum-free eukaryotic cell culture medium supplement and the generated thereof. The invention further provides a method of making a serum-free, eukaryotic cell culture medium and the medium generated thereof. The invention further provides a kit containing the medium of the invention. The invention also provides a method of expanding CD34<+> hematopoietic cells and a composition comprising CD34<+> hematopoietic cells in a serum-free, eukaryotic cell culture medium of the invention.
    Type: Grant
    Filed: May 10, 2012
    Date of Patent: June 24, 2014
    Assignee: Sino-Cell Technologies
    Inventors: Shiaw-Min Hwang, Chi-Hsien Liu, Chao-Ling Yao, I-Ming Chu, Tzu-Bou Hsieh
  • Publication number: 20120282237
    Abstract: The present invention relates to the manipulations of expression levels or enzymatic activities of PRMTs differentially regulate hematopoietic differentiation toward megakaryocytes or erythrocytes. The modulation of lineage fates of megakaryocyte-erythrocyte progenitors is useful for various purposes, including particular regulation of hematopoiesis for therapy and manufacture of individual blood cells for transplantation.
    Type: Application
    Filed: May 3, 2011
    Publication date: November 8, 2012
    Inventors: Wey-Jinq LIN, Yuan-I Chang, Wei-Kai Hua, Yi-Chi Hung, Chih-Jen Kuan, Jiun-Shyang Leou, Chao-Ling Yao, Shiaw-Min Hwang
  • Publication number: 20120220032
    Abstract: The present invention provides a method of determining the optimal composition of a serum-free, eukaryotic cell culture medium supplement, using 2-level factorial design and the steepest ascent method. The invention further provides a method of making a serum-free eukaryotic cell culture medium supplement and the generated thereof. The invention further provides a method of making a serum-free, eukaryotic cell culture medium and the medium generated thereof. The invention further provides a kit containing the medium of the invention. The invention also provides a method of expanding CD34<+> hematopoietic cells and a composition comprising CD34<+> hematopoietic cells in a serum-free, eukaryotic cell culture medium of the invention.
    Type: Application
    Filed: May 10, 2012
    Publication date: August 30, 2012
    Applicant: SINO CELL TECHNOLOGIES INC.
    Inventors: Shiaw-Min Hwang, Chi-Hsien Liu, Chao-Ling Yao, I-Ming Chu, Tzu-Bou Hsieh
  • Publication number: 20100227401
    Abstract: Disclosed herein are media and processes for the ex vivo production of megakaryocytes from human CD34+ cells, in which human CD34+ cells, either being freshly isolated from a newborn's cord blood or having been subcultured in an expansion medium after isolation from a newborn's cord blood, are subjected to cultivation in a cultivating medium consisting essentially of a basal medium, a serum substitute and a cytokine cocktail, wherein the serum substitute consists of human serum albumin, insulin, and transferrin; and the cytokine cocktail consists of thrombopoietin (TPO), stem cell factor (SCF), Flt-3 ligand (FL), interleukin-3 (IL-3), IL-6, IL-9, and granulocyte-macrophage colony-stimulating factor (GM-CSF).
    Type: Application
    Filed: April 5, 2010
    Publication date: September 9, 2010
    Applicant: FOOD INDUSTRY RESEARCH AND DEVELOPMENT INSTITUTE
    Inventors: Chao-Ling YAO, Te-Wei CHEN, Shiaw-Min HWANG
  • Patent number: 7723106
    Abstract: A stroma-free, serum-free, and chemically defined medium and a method for mononuclear cell expansion ex vivo using the same. An exemplary medium includes a basal medium, a serum substitute, and a cytokine formula.
    Type: Grant
    Filed: July 29, 2005
    Date of Patent: May 25, 2010
    Assignee: Food Industry Research & Development Institute
    Inventors: Shiaw-Min Hwang, Chao-Ling Yao, Tzu-Bou Hsieh, I-Ming Chu
  • Publication number: 20080248515
    Abstract: The present invention provides a method of determining the optimal composition of a serum-free, eukaryotic cell culture medium supplement, using 2-level factorial design and the deepest ascent method. The invention further provides a method of making a serum-free eukaryotic cell culture medium supplement and the generated thereof. The invention further provides a method of making a serum-free, eukaryotic cell culture medium and the medium generated thereof. The invention further provides a kit containing the medium of the invention. The invention also provides a method of expanding CD34<+> hematopoietic cells and a composition comprising CD34<+> hematopoietic cells in a serum-free, eukaryotic cell culture medium of the invention.
    Type: Application
    Filed: May 19, 2008
    Publication date: October 9, 2008
    Applicant: SINO CELL TECHNOLOGIES INC.
    Inventors: Shiaw-Min Hwang, Chi-Hsien Liu, Chao-Ling Yao, I-Ming Chu, Tzu-Bou Hsieh
  • Publication number: 20070207544
    Abstract: A stroma-free, serum-free, and chemically defined medium and a method for mononuclear cell expansion ex vivo using the same. An exemplary medium includes a basal medium, a serum substitute, and a cytokine formula.
    Type: Application
    Filed: May 10, 2007
    Publication date: September 6, 2007
    Applicant: FOOD INDUSTRY RESEARCH & DEVELOPMENT INSTITUTE
    Inventors: Shiaw-Min Hwang, Chao-Ling Yao, Tzu-Bou Hsieh, I-Ming Chu
  • Publication number: 20060024827
    Abstract: A stroma-free, serum-free, and chemically defined medium and a method for mononuclear cell expansion ex vivo using the same. An exemplary medium includes a basal medium, a serum substitute, and a cytokine formula.
    Type: Application
    Filed: July 29, 2005
    Publication date: February 2, 2006
    Inventors: Shiaw-Min Hwang, Chao-Ling Yao, Tzu-Bou Hsieh, I-Ming Chu
  • Publication number: 20050032122
    Abstract: The present invention provides a method of determining the optimal composition of a serum-free, eukaryotic cell culture medium supplement, using 2-level factorial design and the deepest ascent method. The invention further provides a method of making a serum-free eukaryotic cell culture medium supplement and the generated thereof. The invention further provides a method of making a serum-free, eukaryotic cell culture medium and the medium generated thereof. The invention further provides a kit containing the medium of the invention. The invention also provides a method of expanding CD34<+> hematopoietic cells and a composition comprising CD34<+> hematopoietic cells in a serum-free, eukaryotic cell culture medium of the invention.
    Type: Application
    Filed: August 3, 2004
    Publication date: February 10, 2005
    Inventors: Shiaw-Min Hwang, Chi-Hsien Liu, Chao-Ling Yao, I-Ming Chu, Tzu-Bou Hsieh