Abstract: Provided are methods of producing an acellular organ. The method includes the steps of, subjecting an organ derived from an animal to a static supercritical fluid (SCF) treatment followed by a dynamic SCF treatment. Optionally, the method of the present disclosure further includes a hypertonic and a hypotonic treatments prior to the static SCF treatment, and/or a neutralizing treatment after the dynamic SCF treatment. Also disclosed herein are acellular organs produced by the present method.

Abstract: Provided are methods of producing an acellular organ. The method includes the steps of, subjecting an organ derived from an animal to a static supercritical fluid (SCF) treatment followed by a dynamic SCF treatment. Optionally, the method of the present disclosure further includes a hypertonic and a hypotonic treatments prior to the static SCF treatment, and/or a neutralizing treatment after the dynamic SCF treatment. Also disclosed herein are acellular organs produced by the present method.

Abstract: A method for producing biodiesel includes feeding methanol into a tank and heating methanol to a temperature above a critical temperature of methanol to obtain a supercritical methanol. A reactive matrix is fed into the tank. The reactive matrix includes acetic acid and a material oil. A molar ratio of acetic acid to the material oil is in a range from 1:1 to 5:1. A molar ratio of methanol to the material oil is in a range from 20:1 to 90:1. A supercritical carbon dioxide is fed into the tank. A total pressure of the supercritical methanol and the supercritical carbon dioxide is higher than 10 MPa. The supercritical methanol, the material oil, acetic acid, and the supercritical carbon dioxide react with each other in the tank at 240-500° C. and 8.1-50 MPa for 0.1-1.5 hours to obtain biodiesel.