Abstract: Cyanophage Syn5 RNA polymerase (RNAP) and methods of purifying it are provided. Cyanophage Syn5 RNAP having one or more promoter mutations are provided. Methods of expressing cyanophage Syn5 RNAP are provided. Novel promoter sequences and in vitro transcription systems utilizing Syn5 RNAP are provided. Methods of making high range ssRNA ladders are also provided. Methods of incorporating 2?-modified dNTPs using Syn5 RNA polymerase are provided.

Abstract: Methods of in vitro transcription using cyanophage Syn5 RNA polymerase (RNAP) or mutants thereof and transcription conditions are provided.

Abstract: Cyanophage Syn5 RNA polymerase (RNAP) and methods of purifying it are provided. Cyanophage Syn5 RNAP having one or more promoter mutations are provided. Methods of expressing cyanophage Syn5 RNAP are provided. Novel promoter sequences and in vitro transcription systems utilizing Syn5 RNAP are provided. Methods of making high range ssRNA ladders are also provided. Methods of incorporating 2?-modified dNTPs using Syn5 RNA polymerase are provided.

Abstract: Methods of in vitro transcription using cyanophage Syn5 RNA polymerase (RNAP) or mutants thereof and transcription conditions are provided.

Abstract: A method of amplifying a template DNA molecule comprising incubating the template DNA molecule in a reaction mixture comprising a DNA polymerase and at least one accessory protein at a constant temperature to produce amplified product, wherein production of amplified product does not require exogenously-added oligonucleotide primers and the template DNA molecule does not have have terminal protein covalently bound to either 5? end.

Abstract: Methods and kits for rendering inert contaminating nucleic acids are provided. The methods and kits include an enzyme capable of digesting the contaminating nucleic acids, an activator which activates the enzyme, and an inactivating agent capable of binding to or displacing the activator from the enzyme thus rendering the enzyme inactive.

Abstract: Chimeric DNA polymerase having a DNA polymerase domain and processivity factor binding domain not naturally associated with DNA polymerase domain.

Abstract: Method for treatment of an infection in an animal or plant by an organism having a non-discriminating DNA polymerase. The organism is contacted with a therapeutically effective amount of a dideoxynucleoside or dideoxynucleotide in a pharmaceutically acceptable buffer. Such contact reduces the infection or a symptom of the infection in the animal or plant.

Abstract: A method for sequencing a strand of DNA, including the steps of: providing the strand of DNA; annealing the strand with a primer able to hybridize to the strand to give an annealed mixture; incubating the mixture with a deoxyribonucleoside triphosphate, a DNA polymerase, and a chain terminating agent under conditions in which the polymerase causes the primer to be elongated to form a series of DNA products differing in length of the elongated primer, each DNA product having a chain terminating agent at its elongated end; the number of each DNA product being approximately the same for substantially all DNA products differing in length from 1 to 20 bases.

Abstract: This invention relates to methods for determining the nucleotide base sequence of a deoxyribose nucleic acid molecule comprising the steps of incubating the nucleic acid molecule with an oligonucleotide primer of 5 to 8 bases in length, a plurality of deoxynucleoside triphosphates, at least one chain terminating agent, and a T7-type DNA polymerase having less than 500 units of exonuclease activity under conditions in which the primer is extended until the chain terminating agent is incorporated, and separating the products of the incubating step according to size, whereby at least a part of the nucleotide base sequence of the nucleic acid molecule can be determined.

Abstract: Method of nucleic acid hybridization for detecting the presence of a specific nucleic acid sequence in a population of different nucleic acid sequences using a nucleic acid probe. The nucleic acid probe hybridizes with the specific nucleic acid sequence but not with other nucleic acid sequences in the population. The method includes contacting a sample (potentially including the nucleic acid sequence) with the nucleic acid probe under hybridizing conditions in the presence of a single-stranded DNA binding protein provided in an amount which stimulates renaturation of a dilute solution (i.e., one in which the t.sub.1/2 of renaturation is longer than 3 weeks) of single-stranded DNA greater than 500 fold (i.e., to a t.sub.1/2 less than 60 min, preferably less than 5 min, and most preferably about 1 min.) in the absence of nucleotide triphosphates.

Abstract: A kit or solution for use in extension of an oligonucleotide primer having a first single-stranded region on a template molecule having a second single-stranded region homologous to the first single-stranded region, comprising a first agent able to cause extension of the first single-stranded region of the primer on the second single-stranded region of the template in a reaction mixture, and a second agent able to reduce the amount of pyrophosphate in the reaction mixture below the amount produced during the extension in the absence of the second agent.

Abstract: A method for sequencing a strand of DNA, including the steps off: providing the strand of DNA; annealing the strand with a primer able to hybridize to the strand to give an annealed mixture; incubating the mixture with four deoxyribonucleoside triphosphates, a DNA polymerase, and at least three deoxyribonucleoside triphosphates in different amounts, under conditions in favoring primer extension to form nucleic acid fragments complementory to the DNA to be sequenced; labelling the nucleic and fragments; separating them and determining the position of the deoxyribonucleoside triphosphates by differences in the intensity of the labels, thereby to determine the DNA sequence.

Abstract: This invention relates to T7-type DNA polymerases and methods for using them.

Abstract: This invention relates to T7-type DNA polymerases and methods for using them.

Abstract: Methods for producing blunt-ended double stranded DNA, for labelling the 3'-end of a DNA fragment, and for in vitro mutagenesis of a DNA fragment. A processive DNA polymerase is used in each method.

Abstract: An automated DNA sequencing apparatus having a reactor for providing at least two series of DNA products formed from a single primer and a DNA strand, each DNA product of a series differing in molecular weight and having a chain terminating agent at one end; separating means for separating the DNA products to form a series bands, the intensity of substantially all nearby bands in a different series being different, band reading means for determining the position anThis invention was made with government support including a grant from the U.S. Public Health Service, contract number AI-06045. The U.S. government has certain rights in the invention.

Abstract: This invention relates to processive DNA polymerases and methods for using them.

Abstract: A method for sequencing a strand of DNA, including the steps of: providing the strand of DNA; annealing the strand with a primer able to hybridize to the strand to give an annealed mixture; incubating the mixture with a deoxyribonucleoside triphosphate, a DNA polymerase, and a chain terminating agent under conditions in which the polymerase causes the primer to be elongated to form a series of DNA products differing in length of the elongated primer, each DNA product having a chain terminating agent at its elongated end; the number of each DNA product being approximately the same for substantially all DNA products differing in length from 1 to 20 bases.

Abstract: 1Method for production of a composition consisting essentially of a T7-type DNA polymerase and thioredoxin. The method includes culturing a cell containing plasmid DNA encoding a T7-type DNA polymerase to express the T7-type DNA polymerase from the plasmid DNA, and purifying the T7-type DNA polymerase expressed from the cell to reduce the exonuclease activity associated with the T7-type DNA polymerase compared to the level of exonuclease activity associated with a corresponding naturally-occurring T7-type DNA polymerase.