Abstract: Reported herein are novel recombinant respiratory syncytial viruses (RSV) having an attenuated phenotype in which the native positions of the NS1 and/or NS2 genes in the RSV genome are shifted to a higher position, that is at positions that are more distal to the promoter. The changes in the gene positions may be present in combination with mutations at other loci to achieve desired levels of attenuation and immunogenicity. The recombinant RSV strains described here are suitable for use as live-attenuated RSV vaccines. Also provided are polynucleotide sequences capable of encoding the described viruses, as well as methods for producing and using the viruses.

Abstract: A seal section for use in a downhole submersible pumping system includes a lower chamber, an upper chamber and an intermediate guide section between the lower chamber and the upper chamber. The lower chamber includes a seal bag assembly that has a seal bag containing motor oil and an exterior space around the seal bag that contains high density oil that is denser than the motor oil. The upper chamber includes a volume of the high density oil at the bottom of the upper chamber and a gravity trap connected to the exterior space through an intermediate passage in the intermediate guide section. The gravity trap includes an opening submerged in the volume of high density oil in the upper chamber.

Abstract: A gas mitigation system for controlling the amount of gas that reaches a submersible pumping system deployed in a wellbore includes a well zone isolation device disposed in the wellbore between the submersible pumping system and a gas collecting region. The gas mitigation system further includes a back pressure control module and a gas vent line extending from the gas collecting region through the well zone isolation device to the back pressure control module. A liquid intake line extends from the well zone isolation device to an area of the wellbore upstream from the gas collecting region.

Abstract: Systems and methods for monitoring and suppressing a back EMF generated by an ESP motor when the ESP system is moved in a well. Embodiments provide both electrical braking to reduce the rotation of the motor (and corresponding back EMF) and warnings to alert users to hazardous conditions caused by the back EMF. One embodiment includes an ESP system having a permanent magnet motor, a back EMF safety system, and a power cable connected between them. The proximal ends of the cable's conductors are connected terminals of the back EMF safety system, which has switches that are closed to short circuit the conductors so that back EMF currents from rotation of the motor induce electrical braking in the motor. Currents and phase-to-phase voltages on the conductors are monitored to detect overcurrent and overvoltage conditions that may trigger alarms or other actions.

Abstract: A gas mitigation system for controlling the amount of gas that reaches a submersible pumping system deployed in a wellbore includes a well zone isolation device disposed in the wellbore between the submersible pumping system and a gas collecting region. The gas mitigation system further includes a back pressure control module and a gas vent line extending from the gas collecting region through the well zone isolation device to the back pressure control module. A liquid intake line extends from the well zone isolation device to an area of the wellbore upstream from the gas collecting region.

Abstract: A computing system may include an object representation engine and an object incarnation engine. The object representation engine may be configured to define a computer-aided design (CAD) object in a CAD model as a combination of an object boundary comprised of bounding faces that encapsulate the CAD object and a microstructure that defines an internal geometry of the CAD object in a procedural representation. The procedural representation may be a representation of the internal geometry of the CAD object in a non-incarnated form. The object incarnation engine may be configured to incarnate, via the procedural representation of the microstructure, the internal geometry of the CAD object into a geometric representation to perform a CAD operation on the CAD object.

Abstract: A gas mitigation system for controlling the amount of gas that reaches a submersible pumping system deployed in a wellbore includes a well zone isolation device disposed in the wellbore between the submersible pumping system and a gas collecting region. The gas mitigation system further includes a back pressure control module and a gas vent line extending from the gas collecting region through the well zone isolation device to the back pressure control module. A liquid intake line extends from the well zone isolation device to an area of the wellbore upstream from the gas collecting region.

Abstract: Apparatus and methods for analyzing samples, including for example, samples containing nucleic acids, antibodies, and/or antigens are described. The apparatus may include a frame having a cartridge receiver, two optical devices, thermal cycler, lysis assembly, hybridization heater, and sample transfer assembly. In use, the apparatus may perform multiple sample preparation and analysis processes within the same disposable cartridge including, for example, performing a PCR assay and immunoassay in the same cartridge.

Abstract: A seal section for use in a downhole submersible pumping system includes one or more fluid separation mechanisms, a shaft, a mechanical seal chamber and a mechanical seal inside the mechanical seal chamber. The seal section further comprises a mechanical seal protector around the mechanical seal. The mechanical seal protector includes a cap that surrounds a portion of the mechanical seal and a filter screen. The cap may also include one or more expulsion ports extending through the cap. The cap may be stationary or connected to the shaft and configured for rotation.

Abstract: A submersible pumping system has an electric motor, a rotary hydraulic pump driven by the electric motor, and a linear hydraulic pump that is configured to move a production fluid. The rotary hydraulic pump produces a pressurized working fluid that drives the linear hydraulic pump. In another aspect, a method is disclosed for controlling the temperature of an electric motor within a submersible pumping system disposed in a wellbore. The method includes the steps of circulating motor lubricant through a hydraulically driven production pump to reduce the temperature of the motor lubricant.

Abstract: A method for representing a product using data in different data processing formats comprises deriving data relating to one or more first parts of the product in a first format and deriving data relating to one or more second parts of the product in a second format. The first format includes mesh data and the second format includes a classic geometric representation. In a data processing system, at least one first part of the product including mesh data is selected and at least one second part of the product including classic geometric representation data is selected.

Abstract: Methods and systems for magnetic mixing. Particular embodiments relate to applying a magnetic field to move a magnetically responsive component in a chamber.

Abstract: A method of operating a submersible pumping system includes the steps of placing the pumping system in a normal mode of operation and then detecting the possible presence of a gas slug in proximity to the pumping system by measuring a decrease in the load on the motor. Once the possibility of a gas slug has been detected, the method continues by placing the pumping system in a reduced speed cycle. The pumping system is kept in the reduced speed cycle until the absence of a gas slug in proximity to the pumping system is detected by evaluating two independent conditions within the pumping system.

Abstract: Apparatus and methods for analyzing samples, including for example, samples containing nucleic acids, antibodies, and/or antigens are described. The apparatus may include a frame having a cartridge receiver, two optical devices, thermal cycler, lysis assembly, hybridization heater, and sample transfer assembly. In use, the apparatus may perform multiple sample preparation and analysis processes within the same disposable cartridge including, for example, performing a PCR assay and immunoassay in the same cartridge.

Abstract: A submersible pumping system has an electric motor, a rotary hydraulic pump driven by the electric motor, and a linear hydraulic pump that is configured to move a production fluid. The rotary hydraulic pump produces a pressurized working fluid that drives the linear hydraulic pump. In another aspect, a method is disclosed for controlling the temperature of an electric motor within a submersible pumping system disposed in a wellbore. The method includes the steps of circulating motor lubricant through a hydraulically driven production pump to reduce the temperature of the motor lubricant.

Abstract: An adapter is used to connect a first component within a downhole pumping system to a second component within the downhole pumping system. The adapter includes an upstream section adjacent the first component and a downstream section adjacent the second component. The adapter further includes an articulating joint that permits the angular movement of the first component with respect to the second component. The articulating joint includes a flexible metal casing extending between the upstream section and the downstream section.

Abstract: An electric submersible pumping system is configured to pump fluids from a wellbore. The electric submersible pumping system includes a motor filled with dielectric motor lubricant and a pump driven by the motor. The electric submersible pumping system further includes an upper seal section connected to the motor and filled with seal section oil. The electric submersible pumping system also includes a lower seal section connected to the motor. In preferred embodiments, the lower seal section includes an expansible bladder that isolates the dielectric motor lubricant from wellbore fluids. The upper seal section is in fluid isolation from the motor. The lower seal section is in fluid communication with the motor.

Abstract: A seal section for use in a downhole submersible pumping system includes one or more fluid separation mechanisms, a shaft and one or more primary mechanical seals. Each of the one or more primary mechanical seals is disposed along the shaft. The seal section also includes one or more redundant shaft seals that each substantially surrounds a corresponding one of the one or more primary mechanical seals. In another aspect, a seal section for use in a pumping system includes one or more fluid separation mechanisms, a shaft, a primary mechanical seal and a redundant shaft seal. The redundant shaft seal substantially surrounds the primary mechanical seal. The seal section further includes an internal seal chamber defined by the annular space between the primary mechanical seal and the redundant shaft seal.

Abstract: Methods and systems for magnetic mixing. Particular embodiments relate to applying a magnetic field to move a magnetically responsive component in a chamber.