Patents by Inventor Charles D. Puglia

Charles D. Puglia has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 5358855
    Abstract: A cyto(histo)chemical technique for determining inosine 5'-monophosphate dehydrogenase (IMPD) presence and concentration within one or more morphologically classified or classifiable cells taken from an animal or a human subject. Cell monolayers are prepared, fixed and stained according to a specified procedure in which inosine 5'-monophosphate (as enzyme substrate), nicotinamide adenine dinucleotide (NAD), phenazine methosulfate (PMS) and nitro blue tetrazolium (NBT) as a diazonium salt together produce a granular, dark blue deposit of formazan generally proportional to the IMPD activity in the cell. Cells are evaluated either visually or by image analysis, with a computerized image analyzer or other imaging system, according to accepted biostatistical methods and standards.
    Type: Grant
    Filed: May 14, 1992
    Date of Patent: October 25, 1994
    Assignee: The Medical College of Pennsylvania
    Inventors: Olivera T. Markovic, Nenad S. Markovic, Jay Roberts, Charles D. Puglia, Svetomir N. Markovic